A5025463709- Cystic Fibrosis Research Advances
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Pleural and Pulmonary Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Metabolism and Genetic Disorders
- Lung Cancer Diagnosis and Treatment
- Gut microbiota and health
- Mitochondrial Function and Pathology
- Retinoids in leukemia and cellular processes
- Pneumothorax, Barotrauma, Emphysema
- Pediatric health and respiratory diseases
- Cardiac Arrest and Resuscitation
- Protease and Inhibitor Mechanisms
- Amino Acid Enzymes and Metabolism
- Free Radicals and Antioxidants
- Neonatal and Maternal Infections
- Genetic Neurodegenerative Diseases
- Carcinogens and Genotoxicity Assessment
- Dysphagia Assessment and Management
- Immune cells in cancer
- Asthma and respiratory diseases
- S100 Proteins and Annexins
German Center for Lung Research
2015-2025
University Hospital Heidelberg
2015-2025
Heidelberg University
2016-2025
European Molecular Biology Laboratory
2018
Heidelberg University
2016
Massachusetts General Hospital
2012
Harvard University
2012
University Hospital Carl Gustav Carus
2012
Society of Paediatric Oncology and Haematology
2006
Zentrum für Kinderheilkunde
2005
Neutrophil elastase (NE) is a key risk factor for severity of cystic fibrosis (CF) lung disease. Recent studies identified increased NE activity on the surface airway neutrophils from CF-like mice and patients with CF. However, role surface-bound in CF disease remains unknown. We determined relationship between CF.Surface-bound was measured sputum 35 eight healthy controls using novel lipidated Förster resonance energy transfer reporters correlated free activity, neutrophil counts,...
Neutrophils are recruited to the airways of individuals with cystic fibrosis (CF). In adolescents and adults CF, airway neutrophils actively exocytose primary granule protease elastase (NE), whose extracellular activity correlates lung damage. During childhood, free NE is measurable only in a subset patients, exocytic function unknown.To measure exocytosis by relation damage children CF.We measured using chest computed tomography coupled Perth-Rotterdam Annotated Grid Morphometric Analysis...
In COPD patients with severe right-sided emphysema, complete major and incomplete minor fissure, implantation of one-way valves in both the right upper (RUL) middle lobes (ML) is a possible approach for endoscopic lung volume reduction. The aim this retrospective analysis was to evaluate response therapy complication rate at 90 days (90d-FU) after combined RUL-ML valve implantation. This retrospective, monocentric study included all from Thoraxklinik Heidelberg who underwent treatment...
Zusammenfassung In diesem Artikel werden die Highlights im Bereich der interventionellen Pneumologie Kongresse Deutschen Gesellschaft für (DGP) März 2024 und Österreichischen (ÖGP) September zusammengefasst. Auch vergangenen Jahr gab es wieder neue Entwicklungen zahlreiche Studien Endoskopie Pneumologie, in den abwechslungsreichen Programmen dieser beiden präsentiert wurden. U. a. wurden dabei bronchoskopische Techniken bei Abklärung einer mediastinalen Lymphadenopathie diskutiert,...
Objectives: To assess the effects of different levels spontaneous breathing during biphasic positive airway pressure/airway pressure release ventilation on lung function and injury in an experimental model moderate acute respiratory distress syndrome. Design: Multiple-arm randomized study. Setting: University hospital research facility. Subjects: Thirty-six juvenile pigs. Interventions: Pigs were anesthetized, intubated, mechanically ventilated. Moderate syndrome was induced by repetitive...
A genuine microbiota resides in the lungs which emanates from colonization by oropharyngeal microbiota. Changes might be source of dysbiosis observed lower airways patients suffering asthma or cystic fibrosis (CF). To examine this hypothesis, we compared throat healthy children (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>62</mml:mn></mml:math>) and that with...
Introduction: Currently, there is a lack of bronchoscopic lung volume reduction options that do not depend on fissure integrity. Endobronchial coils have been extensively studied to address this need but exhibited variable patient response and discontinued. Lung tension device (LTD) represent the next-generation coil treatment. This study aimed evaluate safety, feasibility, efficacy LTD-coil Methods: Patients with advanced emphysema hyperinflation were enrolled at two European sites....
Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into airways. To assess if cathepsin G (CG) is a novel key player in chronic inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The probes quantitatively revealed elevated CG activity samples from 46 patients. For future basic science personalized clinical applications, rapid, highly informative, easily translatable small-molecule...
Airway inflammation and microbiome dysbiosis are hallmarks of cystic fibrosis (CF) lung disease. However, longitudinal studies needed to decipher which factors contribute the long-term evolution these key features CF. We therefore evaluated relationship between fluctuation in inflammatory parameters a study including short- (1-year) (3+ years) period. collected 118 sputum samples from 26 CF adult patients analyzed them by 16S rRNA gene sequencing. measured levels cytokines, neutrophil...
BackgroundMacrophages are the major resident immune cells in human airways coordinating responses to infection and injury. In cystic fibrosis (CF), neutrophils recruited shortly after birth, actively exocytose damaging enzymes prior chronic infection, suggesting a potential defect macrophage immunomodulatory function. Signaling through exhaustion marker programmed death protein 1 (PD-1) controls function cancer, sepsis, airway infection. Therefore, we sought identify associations between...
Background There is increasing interest in Positron Emission Tomography (PET) of 2-deoxy-2-[18F]flouro-D-glucose (18F-FDG) to evaluate pulmonary inflammation during acute lung injury (ALI). We assessed the effect extra-vascular water on estimates 18F-FDG-kinetics parameters experimental and simulated data using Patlak Sokoloff methods, our recently proposed four-compartment model. Methodology/Principal Findings Eleven sheep underwent unilateral lavage 4 h mechanical ventilation. Five...
Hintergrund Bei Patienten mit einem schweren pulmonalen Emphysem kann die endoskopische Lungenvolumenreduktion Ventilen eine reversible Therapieoption zur Reduktion der Symptomlast darstellen. Aktuell sind zwei Ventilsysteme verfügbar: endobronchiale Ventile (EBV) und intrabronchiale (IBV). In vorliegenden Arbeit wurde Effektivität einer kombinierten Implantation von IBV EBV 90 Tage nach Ventilimplantation untersucht.
<b>Small molecule FRET flow cytometry is a new method that enables rapid and sensitive quantification of surface-bound elastase activity on sputum neutrophils from patients with cystic fibrosis potentially other neutrophilic airway diseases</b>http://bit.ly/2IegeSB
Background: Pulmonary disease is the major cause for morbidity and mortality in cystic fibrosis (CF). In CF, forced expiratory volume 1 s (FEV1) referenced against a healthy population (FEV1%predicted) body mass index (BMI) do not allow comparison of severity across age gender. Objectives: We aimed to determine updated FEV1 BMI percentiles patients with CF study their dependence on attrition. Methods: Age- height-adjusted aged 6–50 years were calculated from 4,947 German Registry period...