A5102842093- Aquaculture disease management and microbiota
- Animal Genetics and Reproduction
- Aquaculture Nutrition and Growth
- Meat and Animal Product Quality
- Graphene and Nanomaterials Applications
- Microplastics and Plastic Pollution
- Nanoparticles: synthesis and applications
- Protein Hydrolysis and Bioactive Peptides
- Electrospun Nanofibers in Biomedical Applications
University of Milan
2023-2025
Microplastics (MPs) in fish can cross the intestinal barrier and are often bioaccumulated several tissues, causing adverse effects. While impacts of MPs on well documented, mechanisms their cellular internalization remain unclear. A rainbow-trout (Oncorhynchus mykiss) platform, comprising proximal distal epithelial cells cultured an Alvetex scaffold, was exposed to 50 mg/L (size 1–5 µm) for 2, 4, 6 h. MP uptake faster RTpi-MI compared RTdi-MI. Exposure microplastics compromised integrity by...
The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues allow for a more sustainable flexible approach. Testing all these options in vivo is still the most robust reliable manner pick best material candidates, but it requires use large numbers animals time-consuming expensive. Therefore, we are developing an vitro platform that can evaluation new ingredients. main aim this work was combine digestion protocol extruded, commercially...
In vitro organotypic cell-based intestinal platforms, able to faithfully recapitulate the complex functions of organ in vivo, would be a great support search for more sustainable feed ingredients aquaculture. We previously demonstrated that proliferation or differentiation rainbow trout cell lines is dictated by culture environment. The aim present work was develop platform can efficiently promote into mature enterocytes. compared four options, seeding RTpiMI line derived from proximal...
Reliable and predictive in vitro models would support the search for new raw materials that can improve current fish diets. We recently developed some rainbow trout (RT) intestinal cell-based organotypic platforms demonstrating platform type modulates degree of cell differentiation achieved here we studied whether such correlates with their response to a prolonged exposure diet rich meal. compared three options, seeding RTpiMI RTdiMI lines derived respectively from proximal or distal...