A reduction in L-arginine availability has been implicated the impairment of endothelium-dependent nitric oxide (NO)-mediated vasodilation by ischemia-reperfusion (I/R). However, mechanisms contributing to dysregulation pool remain unknown. Because endothelial cells can metabolize via two major enzymes, that is, NO synthase (NOS) and arginase, we hypothesized up-regulation arginase during I/R reduces NOS thus impairs NO-mediated vasodilation. To test this hypothesis, a local was produced...

Hydrogen peroxide, a relatively stable reactive oxygen species, is known to elicit vasodilation, but its underlying mechanism remains elusive. Here, we examined the role of endothelial nitric oxide (NO), prostaglandin, cytochrome P-450-derived metabolites, and smooth muscle potassium channels in coronary arteriolar dilation abluminal H 2 O . Pig subepicardial arterioles (50–100 μm) were isolated pressurized without flow for vitro study. Arterioles developed basal tone dilated dose...

Objective— Overproduction of reactive oxygen species such as hydrogen peroxide (H 2 O ) has been implicated in various cardiovascular diseases. However, mechanism(s) underlying coronary vascular dysfunction induced by H is unclear. We studied the effect on dilation arterioles to endothelium-dependent and endothelium-independent agonists. Methods Results— Porcine were isolated pressurized without flow for vitro study. All vessels developed basal tone dilated dose-dependently activators nitric...

Tilapia lake virus (TiLV) poses a major threat to global tilapia aquaculture and contributes significant economic losses due the absence of effective vaccines treatments. Given high mortality rates severe pathological effects TiLV on tilapia, alternative strategies, such as immunoglobulin-based therapies, are being considered for disease control. In this study, we developed specific immunoglobulin Y (IgY) antibodies against evaluated their neutralization activity. Laying hens were immunized...

Background and Aim: Although cats are not natural hosts for heartworm infections (Dirofilaria immitis), evidence suggests that feline disease can be detrimental because of a severe inflammatory response. Recent studies have found infection with bacteria the genus Wolbachia is principal cause acute filaria disease; nonetheless, prevalence naturally infected heartworms remains unclear. This study aimed to estimate current distribution its association in pet semi-domesticated Bangkok, Thailand....

Studies in cultured endothelium implicate that lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) or Fcγ receptor II (CD32) contributes to the proatherogenic effects of C-reactive protein (CRP). However, identity receptors linking deleterious actions CRP vasomotor regulation remains unknown.We tested hypothesis LOX-1 adverse on endothelium-dependent function resistance arterioles.Porcine coronary arterioles were isolated for vasoreactivity study, dihydroethidium fluorescence...

The relationship between overall obesity and fat distribution in dogs the development of heart disease is unclear. In present study we evaluated association clinical by morphometric computed tomography (CT)-based measurements. Body condition score (BCS), modified body mass index (MBMI, kg/m2), waist-to-hock-to-stifle distance ratio (WHSDR), waist-to-ilium wing (WIWDR), waist-to-truncal length (WTLR) were compared with (n = 44) without 43) using receiver operating characteristic (ROC)...

The fabrics coated with TiO 2 photocatalyst and fluoropolymer exhibit good water-repellent antimicrobial properties. can be used in the fabrication of smart gowns scrub suits as protective clothing for medical staff.

Exercise training has been shown to restore vasodilation nitric oxide synthase (NOS) activation in arterioles distal coronary artery occlusion. Because reactive oxygen species are generated during NOS uncoupling and the production of vasodilator H2O2 is increased exercise patients with disease, we proposed that may contribute restoration porcine occlusion model.Left circumflex (LCX) miniature swine was progressively occluded for 8 weeks followed by (EX; 5 days/wk treadmill) or sedentary...

Long-term outcomes and survival predictors for different clinicopathologies (idiopathic chronic hepatitis, liver fibrosis, vacuolar hepatopathy) in dogs with hepatobiliary diseases are poorly described. In this study, ninety were followed up to five years investigate clinical factors that predict two-year canine patients after biopsy. Univariate multivariate analyses performed based on laboratory data determine the association between mortality rates. Overall, one-, two-, five-year rates...

Abstract Zoonotic yeast species have been implicated in disease development both humans and cats. This study analyzed the mycobiota present feline facial hair human nails explored potential interspecies associations. A total of 118 biological specimens were examined, including 59 nail samples. DNA extraction sequencing performed to identify specific species. The most predominant cats selected for antifungal susceptibility testing (itraconazole, ketoconazole, miconazole, terbinafine)....

Encephalitozoon cuniculi can cause serious disease and subclinical infection in rabbits requires active surveillance to control the infection. This study investigated association between anti-Encephalitozoon antibody status various health parameters pet rabbits. A total of 90 were divided into healthy (N = 30), clinical 30) groups based on their presentations. The mean ages (37 ± 40 months) (38 34 notably lower compared that group (63 38 months, p < 0.01). Serum titers for antibodies...

Background and Aim: Immunoglobulin (Ig)Y, a specific type of Ig found in chicken eggs, has potential use the diagnosis human animal diseases. This study assessed feasibility using spent laying hens to produce IgY. In addition, effects antigen injection on egg antibody production new were compared. Materials Methods: Hens intramuscularly injected with three booster shots antigens. IgY was extracted from yolks polyethylene glycol 6000 precipitation followed by dialysis. Results: Spent (83...

Background and Aim: Ultrasonography is the first-line method for examining canine liver. Hepatic ultrasound scoring systems are widely described in human medicine, yet there no information on use of semi-quantitative liver diseases. This study aimed to evaluate hepatobiliary scores between physically healthy dogs with primary diseases confirmed by clinical, biochemical, histological parameters. We also evaluated putative correlations ALT or ALP levels. Moreover, severity fold changes enzymes...

The objective of this study was to evaluate the efficacy a non-selective COX inhibitor (tolfenamic acid) and selective COX-2 (robenacoxib) for post-operative pain control in cats. Thirty cats undergoing ovariohysterectomy were randomly divided into three groups: (placebo) group, tolfenamic acid (4 mg/kg/day) robenacoxib (1 group. Non-steroidal anti-inflammatory drugs (NSAIDs) administered orally 2 hr before anesthesia induction 24 48 post-operation. Buccal mucosal bleeding times (BMBTs)...

The sparing effects of tramadol and tramadol-lidocaine infusion on the minimum alveolar concentration (MAC) sevoflurane in dogs as well entropy indices were investigated. Anesthesia was induced eight young, healthy German shepherds weighing 27.6 ± 3.2 kg (mean SD) maintained with sevoflurane. A standard tail-clamp technique used to determine MAC during with: alone measure baseline (MACB); (intravenous loading dose 1.5 mg/kg constant rate [CRI] 2.6 mg/kg/hr; MACT); (tramadol CRI lidocaine...

Abstract Background This study describes the usefulness of allergen-specific Immunoglobulin E (IgE) serology (ASIS) for identifying allergens in dogs with atopic dermatitis. ASIS tests were conducted 23 diagnosed dermatitis indoor (yeast and mites), outdoor (grass pollen, weed tree pollen), fleas. The relationship among positive determined using Pearson’s correlation coefficient (r). Results Of dogs, 26.09%, 4.35%, 47.83% had only allergens, both respectively. prevalence was highest mites...

Background and Aim: Long-haired cats may have an increased risk of dermatophytosis due to insufficient grooming their thick hair coat trapping fungal spores. The prevalence subclinical in long-haired was evaluated using culture Wood's lamp test. Hematology blood chemistry results were compared between negative positive for dermatophytosis. Materials Methods: A total 127 (median age, 3 years [range, 10 months-10 years]) without feline leukemia virus or immunodeficiency infection classified...

Remote vasodilation caused by arteriolar microapplication of acetylcholine cannot be completely attributed to passive cell-cell communication a hyperpolarizing signal. The present study was undertaken ascertain whether neural component may involved in the remote response. In cheek pouch anesthetized hamsters, methacholine (100 microM) applied arteriole micropipette for 5 s, and responses were measured at site application locations: 500 1,000 microm upstream from site. Superfusion with local...