ABSTRACT Swine infectious agents, especially viruses, are potential public health risks associated with the use of pig organs for xenotransplantation in humans. Therefore, there is a need better characterization swine viruses and development diagnostic tests their detection. We report here isolation novel strain porcine circovirus (PCV) from pigs postweaning multisystemic wasting syndrome (PMWS). Affected exhibited severe interstitial pneumonia lymphoid depletion. The complete nucleotide...

Hepatitis E virus (HEV) is a very important public health concern in many developing countries where epidemics of hepatitis are common. Sporadic cases clinical not only occur these but also uncommonly patients with no known epidemiological exposure to HEV industrialized countries. The source infection unknown it has been suggested that animals might serve as reservoir for both settings. We recently identified and characterized an strain (swine HEV) infects large numbers pigs the United...

Evidence of apoptosis was detected for the United States porcine reproductive and respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain VR2385 tissues pigs infected same strain. Apoptosis by agarose gel electrophoresis, transmission electron microscopy terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By double-labelling techniques, primarily uninfected bystander continuous cell line rather than PRRSV-infected cells. In lungs, apoptotic were...

Three groups of 5-week-old cesarian-derived, colostrum-deprived pigs were inoculated intranasally with either a high-virulence isolate (VR2385) or low-virulence (VR2431) porcine reproductive and respiratory syndrome virus (PRRSV) uninfected cell culture media. Formalin-fixed, paraffin-embedded tissues from euthanatized at 10, 21, 28 days post-inoculation examined by in situ hybridization for PRRSV nucleic acid using digoxigenin-labeled antisense RNA probe approximately 1,000 nucleotides...

Background and Aim: According to the previous study, circulating canine parvovirus (CPV) in Thailand is 2a 2b. Nowadays, CPV mutants, including CPV-2c, have been identified many parts of world. This study aimed investigate genetic diversity Thailand. Materials Methods: Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene these was amplified using specific primers polymerase chain reaction (PCR). full-length...

The International Union for the Conservation of Nature and Natural Resources lists green turtle as endangered. Green nesting behavior in Gulf Thailand has decreased to <50% 1995 level. population structure turtles not yet been studied. This study aimed characterize genetic diversity based on comparisons mitochondrial DNA (mtDNA) control region with sequences Indo-Pacific management units (MUs) rookeries, investigate structures, explore phylogeographic relationships. Blood samples (1 mL each)...

Background: Influenza A viruses are capable of crossing the specific barrier between human beings and animals resulting in interspecies transmission.The important factor potential infectivity influenza is suitability receptor binding site host viruses.The affinities avian virus to bind with receptors distributions different.Objective: This study aims investigate anatomical distribution using double staining lectin histochemistry method.Methods: Double was performed identify both SA α2,3 Gal...

Abstract Ruminants are capable of hydrolyzing lignocellulosic residues to absorbable sugars by virtue the microbial communities residing in their rumen. However, large sections such not yet culturable using conventional laboratory techniques. Therefore present study, metagenomic DNA swamp buffalo (Bubalus bubalis) rumen contents was explored culture-independent The consensus regions glycosyl hydrolase 5 (GH5) family cellulases were used as primers for PCR amplification. A full-length...

A 10-year-old, 4.4 kg, intact female poodle presented with clinical signs of weakness in both hind limbs May 2013 at the Veterinary Teaching Hospital, Kasetsart University, Bangkok, Thailand. The dog had a mild fever (39.5°C) and demonstrated hyperesthesia lumbar area. Radiographic imaging showed normal appearance spine intervertebral disk spaces. CBC was within reference ranges. An antipyretic drug, tolfenamic acid, administered once SC (4 mg/kg), combined an analgesic tramadol, PO (3...

Background: The flat‐headed cat ( Prionailurus planiceps ) is a small wild of Southeast Asia and considered extremely endangered. Little known about the hematologic values, blood cell morphology, or hemoparasites this species in relation to other Felidae. Objectives: objective study was report basic values describe light microscopic, cytochemical, ultrastructural characteristics cells 2 wild‐caught cats. In addition, molecular analysis done Hepatozoon organism found neutrophils both Methods:...

Noroviruses (NoVs) are an important human pathogen associated with acute viral gastroenteritis worldwide. NoVs display a significant amount of genetic heterogeneity, making it difficult to develop comprehensive detection assays. In this study, primer sets and probes were designed for TaqMan(®)-based real-time reverse transcription-polymerase chain reaction (RT-PCR) norovirus purposes. The assay was optimized utilized as multiplex RT-PCR genogroup I (GI) detection, singleplex II (GII)...

A banded linsang (Prionodon linsang) presented at our hospital with clinical signs of acute diarrhea. Fecal samples were positive for canine parvovirus (CPV) as determined by polymerase chain reaction primers specific both CPV and feline panleukopenia virus (FPV). The full-length VP2 was cloned, sequenced, compared sequences FPV strains reported in GenBank. amino acids that the host range similar to those FPV. Moreover, acid analysis revealed over 98% homology isolate closely related...

Shiga toxins (Stx) produced by Escherichia coli cause systemic vascular damage that manifests as edema disease in swine and hemolytic uremic syndrome humans. In vitro, Stx inhibit protein synthesis and, depending on circumstances, induce necrosis, apoptosis, or both. The mechanism of vivo Stx-mediated is not known. ability to apoptosis vasculature was studied pigs with oral inoculation Stx-producing E. coli. Arterioles ileum brain were evaluated terminal dUTP nick-end labeling (TUNEL) assay...

The effect of the semi-quantitative expression CD20 in prognosis feline nasal lymphoma has not been described. This study investigated prognostic significance expression, clinicopathological characterization, and treatment outcomes cats with lymphoma. Clinical data from diagnosed were retrospectively collected, including signalment, clinical signs, variables, outcomes, survival times. Using ImageJ software, was semi-quantitatively measured based on proportion CD20-positive areas....

Abstract Objective —To determine the effect of finasteride on programmed cell death (apoptosis) prostatic cells during involution in dogs with benign hypertrophy (BPH). Animals —9 BPH. Procedure —Dogs were randomly assigned to treatment or control groups. Treatment (n = 5) administered (0.1 0.5 mg/kg, PO, q 24 h) for 16 weeks, whereas 4 an inert compound. Prostatic from fluid portion ejaculate and obtained before 1, 2, 3, 4, 8, weeks after initiation treatment. Cells concentrated by use...

Hepatozoon gamonts were observed by light microscopy in neutrophils of a male, wild-caught Leopard Cat. Complete blood counts at presentation and 6 months later unremarkable. Serologic tests negative for both FIV FeLV. A partial sequence the 18S rRNA gene from found cat indicated that, compared with all species examined, protozoan had closest relationship (99.2% similarity) water python (Stegonotus cucullatus). The was clinically healthy last report. Although has been another wild Thailand,...

Canine pyometra, either the closed (closed pyometra [CP]) or open (open [OP]) cervix type, is a frequent uterine disease in intact old age bitches. Therefore, early diagnosis and appropriate medical surgical treatments are crucial to avoid life-threatening condition these This study aimed investigate clinical alterations, blood parameters, causative bacteria, antimicrobial susceptibility, histopathology obtained during aseptic treatment on bitches with pyometra. Sixty of various breeds ages...

The efficacy of antibody detection tools for all stages Ehrlichia canis infections and various genotypes remains unclear. We produced recombinant gp36 (rgp36) antigens from different isolates Thai E. to confirm the immunoreactivities these proteins naturally infected dogs. Sera blood samples were taken 21 dogs with in clinical acute phase ehrlichiosis. expression vectors competent coli two rgp36. These major rgp36s recognized by dogs' sera Western blotting, both anti-dog IgM IgG used as...

The purpose of this study was to survey and compare the amounts elements in serum stranded sea turtles from Gulf Thailand Andaman Sea. had Ca, Mg, P, S, Se, Si concentrations significantly higher than those Ni Pb higher, but not so, Rb detected only Thailand. This may have been related industrial activities Eastern concentration Br Sea were Cu hawksbill (H) olive ridley (O) green be due hemocyanin, as an important component blood crustaceans. Fe for H O chlorophyll, which is chloroplasts eel...