Background: Urethral catheterization in the critical care unit often compromises urinary tract’s defense mechanisms of canine patients and potentially leads to hospital-acquired systemic infection. Clinical signs catheter-associated tract infections (CAUTIs) are frequently absent dogs. Aim: This study aimed evaluate correlation between urinalysis results CAUTIs dogs assess impact prior antibiotic treatment for underlying diseases antibiotic-resistant bacteria. Methods: Twenty-eight underwent...

A conventional PCR (cPCR) remains an effective molecular technique for the diagnosis of canine monocytic ehrlichiosis. However, agarose gel electrophoresis requires additional time after thermal cycling. In present study, we developed a PCR-based lateral flow biosensor (PCR-LFB) to detect Ehrlichia canis (E. canis). Lateral strips allow simple and rapid detection products provide alternative electrophoresis. The sensitivity, specificity, limit PCR-LFB were compared those TaqMan probe-based...

Urethral catheterization in the critical care unit often compromises urinary tract's defense mechanisms of canine patients and potentially leads to hospital-acquired systemic infection. Clinical signs catheter-associated tract infections (CAUTIs) are frequently absent dogs. This study aimed evaluate correlation between urinalysis results CAUTIs dogs assess impact prior antibiotic treatment for underlying diseases antibiotic-resistant bacteria. Twenty-eight underwent urethral Kasetsart...

Rickettsiosis is caused by Orientia spp. and Rickettsia spp., arthropod-borne zoonotic intracellular bacteria. The close relationships between pet dogs, cats owners increase the risk of rickettsial transmission, with limited studies on seroprevalence in pets. This study investigated prevalence rickettsia exposure among dogs Bangkok neighboring provinces. samples from 367 187 used this were leftover serum routine laboratory testing stored at Veterinary Teaching Hospital. In-house...

A 10-year-old, 4.4 kg, intact female poodle presented with clinical signs of weakness in both hind limbs May 2013 at the Veterinary Teaching Hospital, Kasetsart University, Bangkok, Thailand. The dog had a mild fever (39.5°C) and demonstrated hyperesthesia lumbar area. Radiographic imaging showed normal appearance spine intervertebral disk spaces. CBC was within reference ranges. An antipyretic drug, tolfenamic acid, administered once SC (4 mg/kg), combined an analgesic tramadol, PO (3...

Bartonella are arthropod-borne, fastidious, Gram-negative, and aerobic bacilli distributed by fleas, lice, sand flies, and, possibly, ticks. The zoonotic species, henselae clarridgeiae, which the causes of cat scratch disease endocarditis in humans, have been reported from cats, humans Australia. However, to date, there has no report B. or clarridgeiae Australian wild animals their ectoparasites. were detected fleas (Ctenocephalides felis) red foxes (Vulpes vulpes), an introduced pest animal...

(1) Background: Bartonella spp. are Gram-negative, facultative, intracellular bacteria transmitted by hematophagous insects. Several species cause zoonotic diseases such as cat-scratch disease. henselae and clarridgeiae the main found in Thailand, however, there have been few studies on In addition, hematological evaluation of Bartonella-infected animals is limited Thailand. The aims this study were prevalence investigation dogs cats residing Bangkok, (2) Methods: total, 295 513 molecularly...

Urethral catheterization, a common procedure in canine patients, often compromises urinary tract defenses, leading to bacterial colonization and potential systemic infection. Despite posi-tive urine cultures, clinical signs of infection (UTI) may be absent catheterized dogs. This study evaluated the correlation between urinalysis results catheter-associated UTIs hospitalized dogs, considering prior antibiotic treatment resistance. Twen-ty-eight dogs at Kasetsart University Veterinary...

The efficacy of antibody detection tools for all stages Ehrlichia canis infections and various genotypes remains unclear. We produced recombinant gp36 (rgp36) antigens from different isolates Thai E. to confirm the immunoreactivities these proteins naturally infected dogs. Sera blood samples were taken 21 dogs with in clinical acute phase ehrlichiosis. expression vectors competent coli two rgp36. These major rgp36s recognized by dogs' sera Western blotting, both anti-dog IgM IgG used as...

Background: The Sanger sequencing technique has been questioned and challenged by advanced high-throughput approaches. seems to be an obsolete technology. However, there are still research problems that could answered using the Fastidious obligate anaerobic bacteria mostly associated with abscesses in animals. These difficult isolate from frequently excluded due bias of conventional bacterial culturing. Aim: This study demonstrated usefulness a broad-range polymerase chain reaction (PCR)...

Background and Aim: The association between bacterial DNA in stifle joints, including those with cranial cruciate ligament rupture (CCLR) medial patellar luxation (MPL), osteoarthritis dogs remains elusive. This study investigated the potential detection of using a broad-range polymerase chain reaction technique targeting 16S ribosomal RNA gene. Materials Methods: Synovial fluid (35 samples) knee tissue samples (32 were obtained from 35 diagnosed CCLR (n = 20; 11 males nine females) or MPL...

Background and Aim: Because of the diversity local genotypes Ehrlichia canis, genes targeted by TaqMan real-time polymerase chain reaction (RT-PCR) assays should be systematically evaluated. This study evaluated amplification efficiency, linearity, precision, sensitivity two RT-PCR targeting dsb gltA loci E. canis in recombinant plasmids naturally infected dogs. Materials Methods: Thirty blood samples were collected from dogs showing clinical signs canine monocytic ehrlichiosis at Kasetsart...

The efficacy of existing vaccines against Thai strains Rhipicephalus (Boophilus) microplus remains to be determined, and these vaccine antigens should compared homologues derived from local strains. purpose this study was compare Bm95 R. the previously reported sequence Argentinean ticks. mRNA isolated midgut samples, cDNA amplified with Bm95‐specific primers. protein sequences were 94 92% identical, respectively, Bm95. Although similar Bm95, there 45‐amino acid differences among homologues....