Tilapia lake virus (TiLV) now affects Nile tilapia culture worldwide, with no available commercial vaccine for disease prevention. DNA and recombinant protein-based vaccines were developed tested following viral isolation characterization. The strain isolated from diseased hybrid red (

Ruminant animals rely on the activities of β-glucosidases from residential microbes to convert feed fibers into glucose for further metabolic uses. In this report, we determined structures Br2, which is a glycoside hydrolase family 1 β-glucosidase bovine rumen metagenome. Br2 folds classical (β/α)8-TIM barrel domain but displays unique structural features at loop β5→α5 and α-helix 5, resulting in different positive subsites those other GH1 enzymes. exhibited highest specificity toward...

The efficacy of antibody detection tools for all stages Ehrlichia canis infections and various genotypes remains unclear. We produced recombinant gp36 (rgp36) antigens from different isolates Thai E. to confirm the immunoreactivities these proteins naturally infected dogs. Sera blood samples were taken 21 dogs with in clinical acute phase ehrlichiosis. expression vectors competent coli two rgp36. These major rgp36s recognized by dogs' sera Western blotting, both anti-dog IgM IgG used as...

Ixodid ticks have a crucial impact on people and domestic animals worldwide. These parasites also pose serious threat to livestock. To date, vaccination of hosts against is safer, more sustainable alternative chemical control the disease agents they transmit. Because their roles in tick physiology, serpins (serine protease inhibitors) from saliva are among candidates for anti-tick vaccines. Inhibitory employ suicide inhibition mechanism inhibit proteases, where serpin reactive centre loop...

Toxoplasma gondii is recognized as a zoonosis causing toxoplasmosis in animals globally. Cat definitive host of T. and sheds oocyst through feces, which can infect human beings contaminated food ingestion. A precise diagnostic test essential to prevent infection both humans animals. This study aimed develop evaluate the pETite-dense granule antigen 7(GRA7)-based indirect enzyme-linked immunosorbent assay (ELISA) detect cats. gondii-GRA7 was cloned expressed Expresso®small ubiquitin-related...

Inhibition of proteases shows therapeutic potential. Our previous studies demonstrated the cardioprotection by Secretory Leukocyte Protease Inhibitor (SLPI) against myocardial ischaemia/reperfusion (I/R) injury. However, it is unclear whether cardioprotective effect SLPI seen in our works due to inhibition protease enzymes. Several demonstrate that anti-protease independent activity could provide benefits. Here, we show for first time recombinant protein deficient mutant (L72K, M73G, L74G)...

Rhipicephalus (Boophilus) microplus is one of the most widespread ticks causing a massive loss to livestock production. The long-term use acaracides rapidly develops acaracide resistance. In R. microplus, enhancing metabolic activity glutathione S-transferase (RmGST) mechanisms underlying RmGST catalyzes conjugation (GSH) insecticides an easy-to-excrete conjugate. active dimer contains two sites (hydrophobic co-substrate binding site (H-site) and GSH (G-site)) in each monomer. To preserve...

Abstract β -Glucosidases play an important role in biomass degradation as they hydrolyze cellobiose to glucose a final step of cellulolysis. In particular, ruminant animals rely on -glucosidases from rumen microorganisms for conversion plant cellulosic materials into glucose. this study, we are interested characterization novel -glucosidase microorganisms. However, most obligate anaerobes, which require special cultivation conditions. Presently, the metagenomic techniques, enable isolation...

Cattle ticks, Rhipicephalus (Boophilus) microplus, have a serious impact on livestock worldwide. For tick control, host vaccination is more sustainable than chemical. Salivary serpin (serine protease inhibitor) from R. microplus (RmS-3) potential anti-tick vaccine candidate antigen. Like other serpins, RmS-3 has suicide inhibition mechanism, where the reactive centre loop (RCL) cleaved and then inserted into main β-pleated sheet. This causes breakdown of two regions (core domain...

The bovine rumen contains a large consortium of residential microbes that release variety digestive enzymes for feed degradation. However, the utilization these microbial is still limited because microorganisms are mostly anaerobes and thus unculturable. Therefore, we applied sequence-based metagenomic approach to identify novel 2,445-bp glycoside hydrolase family 3 β-glucosidase gene known as BrGH3A from metagenome ruminal fluid. 92-kDa polypeptide composed 814 amino acid residues. Unlike...

Rhipicephalus (Boophilus) microplus is tick parasite that affects the cattle industry worldwide. In R. (B.) microplus, acaricide resistance develops rapidly against many commercial acaricides. One of main strategies to enhance metabolic detoxification mediated by glutathione-S-transferase (RmGST). RmGST detoxifies acaricides catalyzing conjugation glutathione Although structural and dynamic details are expected elucidate biologic activity this molecule, these data have not been available...

Importance of the work: Trypanosoma evansi is a vector-borne pathogen, responsible for T. infection (surra) which has major impact on animal health in Southeast Asia, including Thailand.Objectives: To update current situation regarding trypanosome infections cattle and buffaloes Sakon Nakhon province, Thailand.Materials & Methods: Blood samples were collected from (455) beef (1,077) dairy (227) eight districts province tested using indirect-enzyme-linked immunosorbent assay (ELISA) TRYP1...

Canine parvovirus (CPV) is one of the most common viral infections in dogs, causing acute hemorrhagic gastroenteritis and high mortality. Vaccination effectively prevents CPV infection. However, currently available vaccines have concerns such as maternal immunity interference, shedding virus vaccine, false-positive result based on polymerase chain reaction after vaccination. A subunit vaccine can overcome these problems. This study aimed to express recombinant 35 kDa fragment VP2 protein...