A5004266698- Microbial Inactivation Methods
- Magnetic and Electromagnetic Effects
- Algal biology and biofuel production
- Microfluidic and Bio-sensing Technologies
- Pulsed Power Technology Applications
- Plasma Applications and Diagnostics
- Electrohydrodynamics and Fluid Dynamics
- Biodiesel Production and Applications
- Electrostatic Discharge in Electronics
- Particle accelerators and beam dynamics
- Gyrotron and Vacuum Electronics Research
- Transgenic Plants and Applications
- Particle Accelerators and Free-Electron Lasers
- Plant and Biological Electrophysiology Studies
- Semiconductor Quantum Structures and Devices
- Plant tissue culture and regeneration
- 3D Printing in Biomedical Research
- Plant Genetic and Mutation Studies
- Advanced Fiber Laser Technologies
- Mineral Processing and Grinding
- Enzyme Catalysis and Immobilization
- Neuroscience and Neural Engineering
- Listeria monocytogenes in Food Safety
- Cardiac electrophysiology and arrhythmias
- Protein Hydrolysis and Bioactive Peptides
Karlsruhe Institute of Technology
2015-2025
FZI Research Center for Information Technology
2002-2010
FIZ Karlsruhe – Leibniz Institute for Information Infrastructure
2006-2009
Brookhaven National Laboratory
1984-2005
Associated Universities, Inc.
2003
Leipzig University
2002
The application of pulsed electric field (PEF) technology as a non-thermal cell membrane permeabilization treatment, was widely demonstrated to be effective in microbial inactivation studies, well increase the rates heat and mass transfer phenomena food biotechnological processes (drying, osmotic freezing, extraction, diffusion). Nevertheless, most published papers on topic do not provide enough information for other researchers assess results properly. A general rule/guidance reporting...
The physical basis of electric impulse fragmentation and its applications to the recycling composite materials are reviewed. method is based on initiation a pulsed discharge inside solid dielectric material. With pulse amplitudes /spl sim/300 kV, material layers sim/2 cm can be punctured. Specific energy deposition, lsim/100 J/cm at GW power level, leads pressure buildup lsim/10/sup 10/ Pa in channel. Pressure waves radially propagating cracks launched into body, which lead separation...
Electroporation can be used as a tool for extracting or introducing molecules from into cell. The most important and promising applications of electroporation in medicine biotechnology are described.
Pulsed electric field (PEF) treatment was evaluated for phycocyanin and proteins extraction from Arthrospira platensis (Spirulina platensis). PEF extractions were performed using different specific energies (28, 56 122 J·ml−1 of suspension) the results compared to with bead milling. At highest PEF-treatment a damage cell morphology could be observed yields (up 85.2 ± 5.7 mg·g−1 48.4 4.4 g·100 g−1 phycocyanins proteins, respectively) obtained at J·ml−1. The increased incubation time after...
In this paper, we demonstrate how pulsed electric field (PEF) treatment of microalgae biomass opens promising downstream processing options for an energetic use algae. The fact that lipid droplets remain intracellular after facilitates selective processing. After separation the water-soluble cell contents, algae lipids can be extracted with adequate solvents. environmental-friendly solvent ethanol results in extraction yield from wet and dry that, on average, is four times higher compared...
Abstract Background The gradual extrusion of water-soluble intracellular components (such as proteins) from microalgae after pulsed electric field (PEF) treatment is a well-documented phenomenon. This could be utilized in biorefinery applications with lipid extraction taking place such an ‘incubation’ period, i.e., post-PEF-treatment step during which the biomass left undisturbed before any further processing. goal this work was to explore how incubation improve extraction. Results...
Seven days old seedlings of Arabidopsis thaliana, suspended in a 0.4 S/m buffer solution were exposed to nanosecond pulsed electric fields (nsPEF) with duration 10 ns, 25 ns and 100 ns. The field was varied from 5 kV/cm up 50 kV/cm. specific treatment energy ranged between J/kg kJ/kg. Due electroporation the plasma membrane plant cells, completely died off, when pulses high applied. But even at highest energies, had no lethal effect on seedlings. An evaluation leaf area 7 after revealed...
With decreasing available land and fresh-water resources, the oceans become attractive alternatives for production of valuable biomass, comparable to terrestrial crops. Seaweed cultivation food, chemicals, fuels is already under intensive development, yet efficient technologies separation major components are still missing. We report a food-grade process extraction proteins from green macroalga, Ulva sp., using high-voltage pulsed electric field (PEF) cell-membrane permeabilization, coupled...
Photoautotrophic microalgae based biorefinery concepts are currently not competitive compared to other established production systems. Therefore, innovative upstream processes need be developed increase the competitiveness of photoautotrophic concepts. Abiotic sub-lethal stress induction via nanosecond pulsed electric field (nsPEF) treatment might a viable process efficiency cultivation. In this work, an increased cell growth after nsPEF was observable. Application highly proliferating cells...
Electric field-induced membrane changes are an important approach in the life sciences. However, developments knowledge and translational applications face problems of reproducibility. Indeed, a quick survey literature reveals lack transparent comprehensive reporting essential technical information many papers. Too published scientific papers do not contain sufficient for proper assessment presented results. The general rule/guidance experimental data should require details on exposure...