A5008072243- Virus-based gene therapy research
- Hepatitis B Virus Studies
- Force Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Viral Infections and Vectors
- Advanced Electron Microscopy Techniques and Applications
- Viral Infections and Outbreaks Research
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
California NanoSystems Institute
2024
University of California, Los Angeles
2021-2024
Abstract Mammalian reovirus (MRV) is the prototypical member of genus Orthoreovirus family Reoviridae . However, lacking high-resolution structures its RNA polymerase cofactor μ2 and infectious particle, limits understanding molecular interactions among proteins RNA, their contributions to virion assembly transcription. Here, we report 3.3 Å-resolution asymmetric reconstruction transcribing MRV in situ atomic models capsid proteins, asymmetrically attached RNA-dependent (RdRp) λ3, RdRp-bound...
ABSTRACT Arenaviruses exist globally and can cause hemorrhagic fever neurological diseases, exemplified by the zoonotic pathogen lymphocytic choriomeningitis virus (LCMV). The structures of individual LCMV proteins or their fragments have been reported, but architectural organization nucleocapsid assembly mechanism remain elusive. Importantly, in situ structure arenavirus fusion protein complex (glycoprotein complex, GPC) as present on virion prior to fusion, particularly with its integral...
Abstract As cryogenic electron microscopy (cryoEM) gains traction in the structural biology community as a method of choice for determining atomic structures biological complexes, it has been increasingly recognized that many complexes behave well under conventional negative-stain tend to have preferential orientation, aggregate or simply mysteriously “disappear” on cryoEM grids, but reasons such misbehavior are not understood, limiting systematic approaches solving problem. Here, we...