A5072400459- Ultrasound and Hyperthermia Applications
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Advanced Electron Microscopy Techniques and Applications
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Micro and Nano Robotics
- Force Microscopy Techniques and Applications
- Microbial Inactivation Methods
- Viral gastroenteritis research and epidemiology
- Electron and X-Ray Spectroscopy Techniques
- Inhalation and Respiratory Drug Delivery
- HVDC Systems and Fault Protection
- Advanced Nanomaterials in Catalysis
- Tracheal and airway disorders
- Atmospheric chemistry and aerosols
- Peroxisome Proliferator-Activated Receptors
- RNA Interference and Gene Delivery
- Protein purification and stability
- Enzyme Structure and Function
- Congenital Heart Disease Studies
- Cynara cardunculus studies
- Antimicrobial Peptides and Activities
Stanford University
2022-2024
Clark University
2023-2024
Baylor College of Medicine
2024
Gansu Agricultural University
2024
University of California, Los Angeles
2020-2023
Zhejiang University of Technology
2023
Yan'an Hospital Affiliated To Kunming Medical University
2022
Kunming Medical University
2022
Nanjing University of Aeronautics and Astronautics
2022
Southwest University
2009-2011
Abstract Sentinel lymph node imaging and biopsy is important to clinical assessment of cancer metastasis, novel non-radioactive lymphographic tracers have been actively pursued over the years. Here, we develop gold molecular clusters (Au 25 ) functionalized by phosphorylcholine (PC) ligands for NIR-II (1000–3000 nm) fluorescence draining nodes in 4T1 murine breast CT26 colon tumor mouse models. The Au-phosphorylcholine (Au-PC) probes exhibit ‘super-stealth’ behavior with little interactions...
Prolonged maintenance of therapeutically-relevant levels broadly neutralizing antibodies (bnAbs) is necessary to enable passive immunization against infectious disease.
Most vaccines require several immunizations to induce robust immunity, and indeed, most SARS-CoV-2 an initial two-shot regimen followed by boosters maintain efficacy. Such a complex series of unfortunately increases the cost complexity populations-scale vaccination reduces overall compliance rate. In rapidly evolving pandemic affected spread immune-escaping variants, there is urgent need develop capable providing durable immunity. this work, single immunization subunit vaccine developed that...
Retinal images are the most intuitive medical for diagnosis of fundus diseases. Low-quality retinal cause difficulties in computer-aided systems and clinical ophthalmologists. The high quality is an important basis precision medicine ophthalmology. In this study, we propose a image enhancement method based on deep learning to enhance multiple low-quality images. A generative adversarial network employed build symmetrical network, convolutional block attention module introduced improve...
Over the past few decades, development of potent and safe immune-activating adjuvant technologies has become heart intensive research in constant fight against highly mutative immune evasive viruses such as influenza, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus (HIV). Herein, we developed a modular saponin-based nanoparticle platform incorporating Toll-like receptor agonists (TLRas) including TLR1/2a, TLR4a, TLR7/8a adjuvants their mixtures....
Coronavirus genomes sequester their start codons within stem-loop 5 (SL5), a structured, 5′ genomic RNA element. In most alpha- and betacoronaviruses, the secondary structure of SL5 is predicted to contain four-way junction helical stems, some which are capped with UUYYGU hexaloops. Here, using cryogenic electron microscopy (cryo-EM) computational modeling biochemically determined structures, we present three-dimensional structures from six coronaviruses. The domain betacoronavirus...
Eukaryotic flagella (synonymous with cilia) rely on a microtubule-based axoneme, together accessory filaments to carryout motility and signaling functions. While axoneme structures are well characterized, 3D ultrastructure of their interface mostly unknown, presenting critical gap in understanding structural foundations eukaryotic flagella. In the flagellum protozoan parasite Trypanosoma brucei (T. brucei), is accompanied by paraflagellar rod (PFR) that supports non-planar necessary for...
A simple, rapid and sensitive CE method has been proposed for the determination of gatifloxacin in biological fluid. This is based on ECL reaction tris(2,2'-bipyridyl)ruthenium(II) occurred end-column detection cell. Under optimal conditions, can be assayed within 10 min over concentration range 5.0x10(-8)-5.0x10(-6) g/mL with theoretical plate numbers 18,000. The intra-day inter-day precision signal intensity migration time shows acceptable reproducibility analysis gatifloxacin. presented...
Abstract Over the past few decades, development of potent and safe immune-activating adjuvant technologies has become heart intensive research in constant fight against highly mutative immune evasive viruses such as influenza, SARS-CoV-2, HIV. Herein, we developed a modular saponin-based nanoparticle platform incorporating toll-like receptor agonists (TLRas) including TLR1/2a, TLR4a, TLR7/8a adjuvants their mixtures. These various TLRa-SNP constructs induce unique acute cytokine...
As cryogenic electron microscopy (cryoEM) gains traction in the structural biology community as a method of choice for determining atomic structures biological complexes, it has been increasingly recognized that many complexes behave well under conventional negative-stain tend to have preferential orientation, aggregate or simply mysteriously "disappear" on cryoEM grids. However, reasons such misbehavior are not understood, which limits systematic approaches solving problem. Here, we...
SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) play a key role in mediating variety of plant biological processes. Currently, the function gene family phytohormonal and abiotic stress treatments grapevine is currently unknown, making it worthwhile to characterize analyze expression this grapevine. In present study, 52 VvSNARE genes were identified predominantly distributed on 18 chromosomes. Secondary structures showed that irregular random coils...
Abstract Electron cryo-tomography (cryo-ET) is a powerful imaging tool that allows three-dimensional visualization of subcellular architecture. During morphological analysis, reliable tomogram segmentation can only be achieved through high-quality data. However, unlike single-particle analysis or subtomogram averaging, the field lacks useful quantitative measurement cellular quality. Currently, most prevalent method to determine resolvability an empirical judgment by experts, which...
Abstract As cryogenic electron microscopy (cryoEM) gains traction in the structural biology community as a method of choice for determining atomic structures biological complexes, it has been increasingly recognized that many complexes behave well under conventional negative-stain tend to have preferential orientation, aggregate or simply mysteriously “disappear” on cryoEM grids, but reasons such misbehavior are not understood, limiting systematic approaches solving problem. Here, we...
Glycolate oxidase is a peroxisomal flavoprotein catalyzing the oxidation of glycolate to glyoxylate and plays crucial metabolic roles in green algae, plants, animals. It could serve as biocatalyst for enzymatic production glyoxylate, fine chemical with wide variety applications perfumery, flavor, pharmaceutical agrochemical industries. However, low catalytic activity native levels active enzyme heterologous expression limit its practical use industrial biocatalysis. Herein, from...
Abstract Most vaccines require several immunizations to induce robust immunity, and indeed, most SARS-CoV-2 an initial two-shot regimen followed by boosters maintain efficacy. Such a complex series of unfortunately increases the cost complexity populations-scale vaccination reduces overall compliance rate. In rapidly evolving pandemic affected spread immune-escaping variants, there is urgent need develop capable providing durable immunity. this work, we developed single immunization subunit...
Coronavirus genomes sequester their start codons within stem-loop 5 (SL5), a structured, 5' genomic RNA element. In most alpha- and betacoronaviruses, the secondary structure of SL5 is predicted to contain four-way junction helical stems, some which are capped with UUYYGU hexaloops. Here, using cryogenic electron microscopy (cryo-EM) computational modeling biochemically-determined structures, we present three-dimensional structures from six coronaviruses. The domain betacoronavirus...