Abstract We demonstrate a noncontact single-beam acoustic trapping method for the quantification of mechanical properties single suspended cell with label-free. Experimentally results show that force in morphological deformation trapped cell. While cancer was an beam focus, changes immobilized were monitored using bright-field imaging. The deformability then compared polystyrene microbead as function applied pressure better understanding relationship between and degree deformation. Cell...

Abstract Advancements in diagnostic systems for metastatic cancer over the last few decades have played a significant role providing patients with effective treatment by evaluating characteristics of cells. Despite progress made prognosis, we still rely on visual analysis tissues or cells from histopathologists, where subjectivity traditional manual interpretation persists. This paper presents development dual diagnosis and tool using an vitro acoustic tweezers platform 50 MHz ultrasonic...

Focused ultrasound (FUS) is a rapidly developing stimulus technology with the potential to uncover novel mechanosensory dependent cellular processes. Since it non-invasive, holds great promise for future therapeutic applications in patients used either alone or as complement boost existing treatments. For example, FUS stimulation causes invasive but not non-invasive cancer cell lines exhibit marked activation of calcium signaling pathways. Here, we identify membrane channel PANNEXIN1 (PANX1)...

In this paper, we report a calibration of acoustic trapping force single-beam tweezer (SBAT) at ultrahigh frequency using micropipette aspiration. The forces (F <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">trapping</sub> ) and the trap stiffness on 5-μm polystyrene microbead for 110-MHz SBAT were measured against known generated from micropipette. (k), which represents F corresponding to displacement (x) center, was results showed that higher...

The role of cell mechanics in cancer cells is a novel research area that has resulted the identification new mechanisms therapy resistance. Single beam acoustic (SBA) tweezers are promising technology for quantification mechanical phenotype cells. Our previous study showed SBA can be used to quantify deformability adherent breast lines. physical properties patient-derived (primary) pre-B acute lymphoblastic leukemia (ALL) involved chemotherapeutic resistance have not been widely...

The impact of high-voltage–high-frequency (HVHF) amplifiers on echo-signal quality is greater with very-high-frequency (VHF, ≥100 MHz) ultrasound transducers than low-frequency (LF, ≤15 transducers. Hence, the bias voltage an HVHF amplifier must be stabilized to ensure stable amplitudes. We propose a bias-voltage stabilizer circuit maintain DC voltages over wide input range, thus reducing harmonic-distortion components echo signals in VHF pulse-echo measurement systems. To confirm...

This paper describes the development of a miniaturized 15-MHz side-looking phased-array transducer catheter. The array features 2-2 linear composite with 64 piezoelectric elements mechanically diced into piece PMN-30%PT single crystal and separated by non-conductive epoxy kerfs at 50-μm pitch, yielding total active aperture 3.2 mm in azimuth direction 1.8 elevation direction, an natural focal depth 8.1 mm. includes backing two front matching layers. A custom flexible circuit connects to...

Tiled modular 2-D ultrasound arrays have the potential for realizing large apertures novel diagnostic applications. This work presents an architecture fabrication of tileable array modules implemented using 1-3 composites high-bandwidth (BW) PIN-PMN-PT single-crystal piezoelectric material closely coupled with high-voltage CMOS application-specific integrated circuit (ASIC) electronics buffering and multiplexing functions. The module, which is designed to be operated as a λ -pitch 1.75-D...

Cancer cells undergo a number of biophysical changes as they transform from an indolent to aggressive state. These changes, which include altered mechanical and electrical properties, can reveal important diagnostic information about disease status. Here we introduce high-throughput, functional technique for assessing cancer cell invasion potential, works by probing the mechanically excitable phenotype exhibited invasive cells. Cells are labeled with fluorescent calcium dye imaged during...

Large aperture ultrasonic arrays can be implemented by tiling together multiple pretested modules of high-density acoustic with closely integrated multiplexing and buffering electronics to form a larger high yield. These modular used implement large 1.75D array apertures capable focusing in elevation for uniform slice thickness along the axial direction which improve image contrast. An important goal is obtaining yield sensitivity while reducing extraneous artifacts. We have been developing...

Ultrasound guided needle biopsy is an important method for collection of breast cancer tissue. In this paper, we report on the design and testing a high-voltage 1 to 64 Multiplexer/Demultiplexer (MUX/De-MUX) integrated circuit (IC) ultrasound-guided applications implemented in CMOS process. The IC intended be incorporated inside designed fit inner diameter 2.38 mm. MUX/De-MUX electronics are made up three parts, including low-voltage 6 decoder, level shifter convert from low voltage high...

Elastography, capable of mapping the biomechanical properties biological tissues, serves as a useful technique for clinicians to perform disease diagnosis and determine stages many diseases. Many acoustic radiation force (ARF) based elastography, including impulse (ARFI) imaging harmonic motion (HMI), have been developed remotely assess elastic tissues. However, due lower operating frequencies these approaches, their spatial resolutions are insufficient revealing stiffness distribution on...

For ultrasonic transducers, a number of equivalent circuit models including KLM, Mason and Butterworth Van Dyke (BVD) have been developed. To allow them to be incorporated into design tools for complex integrated circuits, KLM or are not practical because the discrete components circuits negative values. Therefore, BVD model appears more appropriate but it needs improved resolution high frequency ultrasound system may severely affected by impedance mismatching between transducer system, as...

Cell separation and sorting techniques have been employed biomedical applications such as cancer diagnosis cell gene expression analysis. The capability to accurately measure ultrasonic scattering properties from cells is crucial in making an sorter a reality if ultrasound be used the sensing mechanism well. To assess performance of identifying live single with high-frequency ultrasound, 86-MHz lithium niobate pressfocused single-element acoustic transducer was highfrequency measurement...

In glucose-stimulated insulin secretion (GSIS) of pancreatic β-cells, the rise free cytosolic Ca2+ concentration through voltage-gated calcium channels (VGCCs) triggers exocytosis insulin-containing granules. Recently, mechanically induced pathways were also reported, which utilize ions as a direct regulator exocytosis. this study, we aimed to investigate intracellular responses on HIT-T15 β-cell line upon low-intensity pulsed ultrasound (LIPUS) stimulation and found that induces two...

Ultrasound imaging is a highly valuable tool in human tissues due to its non-invasive and easily accessible nature. Despite advances the field of ultrasound research, conventional transducers with frequencies lower than 20 MHz face limitations resolution for cellular applications. To address this challenge, we employed ultrahigh frequency (UHF) demonstrated their potential applications biomedical engineering, specifically cell acoustic tweezers. The lateral achieved 110 UHF transducer was...

Tiled modular 2D ultrasound arrays have the potential for realizing large apertures novel diagnostic applications. Here we present results of recent work fabrication a tileable array module implemented using 1-3 composite high bandwidth PIN-PMN-PT material closely coupled with voltage CMOS ASICs buffering and multiplexing functions. The transducer is supported by 3D printed conducting acoustically attenuating interposer backing which links to PCB housing interface ASICs. A low temperature...

Power amplifiers are used to trigger the transducers in order generate acoustic waves into desired target. The resolution of ultrasound imaging systems is related with sensitivity so high voltage output signals power required be drive transducers. Therefore, a linear amplifier more attractive choice because its higher dynamic range signals. novel pre-distortion circuit using passive resistor, capacitor and inductor components MOSFET device was designed enhance amplifiers. defined as...

The effect of pulse current (PC) on Ni-W alloy electrodeposition was investigated to fill the trenches with aspect ratio 1:2 for MEMS applications. Rapid deposition top side in trench produced a void electrodeposits direct (DC). Morphology inside strongly influenced by applied density and off-time (t(off)). Enhanced filling phenomena were observed increasing because decreasing concentration gradient between bottom trench. complete achieved 200 mA/cm2 on- 100 ms 300 ms, respectively....

We are developing a high frequency ultrasound imaging array for needle biopsy probe. The goal of this work is to integrate 64 channel linear fabricated using PMN-PT transducer material alongside voltage CMOS ASIC. ASIC designed multiplex the signals from single cable synthetic aperture imaging. In paper we discuss some challenges in development and present initial results. was first assembled layer flex circuit gold bumps silver-loaded epoxy. Pulse/echo data obtained prototype assembly....

This paper describes the design of Class A wideband power amplifier (PA) for very high frequency ultrasonic transducer applications with field-effect transistor (FET). The PA is a one-stage common-source 28 V supply voltage. measured gain 15.8 ~ 17.8 dB in range 100 to 250 MHz and current consumption 0.3 0.4 A. 3rd-order input output inter-modulation points (IIP3 OIP3) are 12.25 dBm 27.25 dBm, respectively noise figure 2.14~2.98 80~120MHz.