A5100376269- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Orbital Angular Momentum in Optics
- Electrostatics and Colloid Interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ultrasound and Hyperthermia Applications
- Bone Tissue Engineering Materials
- Photoacoustic and Ultrasonic Imaging
- Ultrasound Imaging and Elastography
- Cellular Mechanics and Interactions
- Photonic and Optical Devices
- Acoustic Wave Resonator Technologies
- Nanofabrication and Lithography Techniques
- Semiconductor materials and interfaces
- Electrowetting and Microfluidic Technologies
- Nanowire Synthesis and Applications
- Photonic Crystals and Applications
- Vibration and Dynamic Analysis
- Plasmonic and Surface Plasmon Research
- Tissue Engineering and Regenerative Medicine
- Muscle activation and electromyography studies
- Advanced Surface Polishing Techniques
- Single-cell and spatial transcriptomics
- Near-Field Optical Microscopy
Hongik University
2013-2024
Soonchunhyang University
2024
Yonsei University
2024
Kwangwoon University
2011-2023
Ulsan National Institute of Science and Technology
2020-2023
Seoul National University
2023
University of Massachusetts Amherst
2015-2020
Shriners Hospitals for Children - Erie
2013-2016
Harvard University
2015-2016
Massachusetts General Hospital
2015-2016
Abstract Common 2D cell cultures do not adequately represent the functions of 3D tissues that have extensive cell–cell and cell–matrix interactions, as well markedly different diffusion/transport conditions. Hence, testing cytotoxicity in may accurately reflect actual toxicity nanoparticles (NPs) other nanostructures body. To obtain more adequate detailed information about NP–tissue we here introduce a 3D‐spheroid‐culture‐based NP toxicology system. Hydrogel inverted colloidal crystal (ICC)...
A single beam acoustic device, with its relatively simple scheme and low intensity, can trap a lipid droplet in manner similar to optical tweezers. Forces the order of hundreds nanonewtons direct toward focus, within range micrometers. This trapping method, therefore, be useful tool for particle manipulation areas where larger particles or forces are involved.
Abstract We demonstrate a noncontact single-beam acoustic trapping method for the quantification of mechanical properties single suspended cell with label-free. Experimentally results show that force in morphological deformation trapped cell. While cancer was an beam focus, changes immobilized were monitored using bright-field imaging. The deformability then compared polystyrene microbead as function applied pressure better understanding relationship between and degree deformation. Cell...
In a previous paper acoustic radiation force on lipid sphere in 100-MHz focused Gaussian field was calculated to demonstrate the tweezer effect near focus. The theoretical formulation based situation where is centered along beam axis. Given intensity distribution independent of x axis, it then approximated by cylindrical model for sake simplicity. Only axial forces were considered because no lateral exist due an object's symmetry. However, difficult employ same technique more general case...
Abstract Various techniques exerting mechanical stress on cells have been developed to investigate cellular responses externally controlled stimuli. Fundamental mechanotransduction processes about how applied physical forces are converted into biochemical signals often examined by transmitting such through and probing its pathway at levels. In fact, many biomechanics studies performed trapping (or immobilizing) individual cells, either attached solid substrates or suspended in liquid media....
This paper presents experimental results demonstrating the feasibility of high frequency ultrasonic sensing and sorting for screening single oleic acid (lipid or oil) droplets under continuous flow in a microfluidic channel. In these experiments, hydrodynamically focused lipid two different diameters (50 μm 100 μm) are centered along middle channel, which is filled with deionized (DI) water. A 30 MHz lithium niobate (LiNbO3) transducer, placed outside first transmits short pulses to...
Cellular scaffolds made on the basis of inverted colloidal crystals (ICC) provide a unique system for investigation cell–cell interactions and their mathematical description due to highly controllable ordered 3D geometry. Here, we describe three new steps in development ICC cell scaffolds. First, it was demonstrated that layer-by-layer (LBL) assembly with clay/PDDA multilayers can be used modify surface enhance adhesion. Second, complex cellular from adherent non-adherent cells co-existing...
The measurement of cell mechanics is crucial for a better understanding cellular responses during the progression certain diseases and identification cell's nature. Many techniques using optical tweezers, atomic force microscopy, micro-pipettes have been developed to probe manipulate cells in spatial domain. In particular, we recently proposed two-dimensional acoustic trapping method as an alternative technique small particle manipulation. Although may advantages over its applications not...
To study telomere maintenance mechanism (TMM) activation during malignant transformation, we compared neurofibroma (NF) and peripheral nerve sheath tumor (MPNST) in the same patient with type-1 neurofibromatosis (NF1), a total of 20 NF-MPNST pairs NF1 patients. These comparisons minimized genetic bias contrasted only changes associated while subtracting that developed upon transformation normal cells to benign tumor. TGF-β superfamily genes were found activate PAX SOX transcription factors,...
Abstract Non-symmetric dimesogens are composed of two different mesogenic units linked via a flexible spacer. In this study, new type non-symmetric dimesogen has been built through the self-assembly intermolecular hydrogen bonding between appropriately designed H-bond donor (3-cholesteryloxycarbonylpentanoic acid) and acceptor (aromatic mesogen with pyridyl group) moieties. As for covalently dimesogens, several types smectic periodicities observed these H-bonded cholesteryl compounds...
Various food components are known for their health-promoting effects. However, biochemical effects generally evaluated in vitro, and actual vivo effect can vary significantly, depending on metabolic profiles. To evaluate the of liver metabolism antioxidant activity, we have developed a two-compartment microfluidic system that integrates dynamics subsequent activity components. In first compartment device, human enzyme fractions were immobilized inside poly(ethylene glycol) diacrylate (PEGDA)...
Multiparticle-trapping offers diverse opportunities and applications in biotechnology. It can be applied to creating various functional materials or organizing reactive particles. In this paper, we demonstrate that it is possible trap manipulate multi-particles an annular pattern with a 24 MHz focused ring-type single element ultrasound transducer. Acoustic ring useful undertaking biotropism studies due equal-distance condition from the center. Also, could serve as force shield protect...
Abstract Background Immunodeficient mouse models that accept human cell and tissue grafts can contribute greater knowledge to stem research. In this technical report, we used biomaterial implants seeded with genetically engineered stromal cells create several unique microenvironments in a single mouse. The scope of study was focused on CD34 hematopoietic stem/progenitor (HSPC) engraftment differentiation within the microenvironment. Results A model system created using subdermal implant...
We recently investigated the design and fabrication of Langevin-type transducers for therapeutic ultrasound. Effect ultrasonic energy arising from transducer on biological tissue was examined. In this study, set to radiate acoustic mesenchymal stem cells (MSCs) inducing differentiation into cartilage tissue. The average chondrogenic ratio in area 20.82% control group, which no external stimulation given. Shear stress applied MSCs as contrast resulted 42.66% with a 25.92% minimum rate;...
Acoustic tweezers (ATs) are a promising technology that can trap and manipulate microparticles or cells with the focused ultrasound beam without physical contact. Unlike optical tweezers, ATs may be used for in vivo studies because they through tissues. However, previous non-invasive microparticle trapping studies, could only spherical particles, such as beads. Here, we present theoretical analysis of how acoustic traps red blood (RBCs) experimental demonstration. The proposed modeling shows...