Genomics research has not yet been translated into routine practical application in breeding Rosaceae fruit crops (peach, apple, strawberry, cherry, apricot, pear, raspberry, etc.). Through dedicated efforts of many researchers worldwide, a wealth genomics resources accumulated, including EST libraries, genetic and physical maps, QTLs, whole genome sequences. The potential approaches to enhance crop improvement, particularly through marker-assisted (MAB), is enormous, but unfulfilled. U.S....

Abstract A linkage mapping approach was used to identify quantitative trait loci (QTL) associated with day‐neutrality in the commercial strawberry, Fragaria × ananassa (Duch ex Rozier). Amplified Fragment Length Polymorphism (AFLP) markers were build a genetic map population of 127 lines developed by crossing day‐neutral (DN) ‘Tribute’ short‐day (SD) ‘Honeoye’. The genotyped AFLP and 429 single dose restriction fragments (SDRF) placed on consensus 1541 cM 43 groups. Individuals from observed...

Phosphorus (P) deficiency in soil is a major constrain for rice production. An important set of genotypes (landraces, old improved and new varieties) were screened P tolerance two cropping seasons Sri Lanka, 2012. The Ultisol soil, which was collected from plot cultivated with without fertilizer application past 40 years (P0) at the Rice Research Development Institute (RRDI), Bathalagoda, used as potting medium greenhouse trials. Two field trials conducted same plots RRDI. Both P0 P30 (30...

Systematic studies of the relative importance apple traits for U.S. producers to inform breeding programs have been lacking. To fill this gap, a series audience surveys with instant feedback at five producer meetings across United States was conducted. The included in study were fruit crispness, juiciness, firmness, flavor, soluble solids concentration, sugar–acid balance, shelf life retail, freedom from storage disorders, host plant disease resistance, and other tree provided by producer....

Luffa acutangula and L. aegyptiaca are two vegetable species commonly found in South East Asia. is widely grown; however, considered as an underutilized crop. The delimits, phylogenetic positions, the varietal identities of Sri Lanka not known. Thus, present study, we aimed to establish delimits varieties grown using morphometric, organoleptic assessments. We assessed five three aegyptiaca. vegetative reproductive data were collected for morphometric analysis DNA sequence polymorphism makers...

Much of the cost associated with marker discovery for assisted breeding (MAB) can be eliminated if a diverse, segregating population is generated, genotyped, and made available to global community. Herein, we present an example hybrid, wild-derived family octoploid strawberry that used by other programs economically find tag useful genes MAB. A pseudo test cross between two wild species Fragaria virginiana F. chiloensis (FVC 11) was generated evaluated set phenotypic traits. total 106...

Developing new cherry cultivars requires breeders to be aware of existing and emerging needs throughout the supply chain, from producer consumer. Because breeding programs in perennial crop plants like sweet tart cherries require both extended time extensive resources, understanding targeting priority traits is critical improve efficiency programs. This study investigated relative importance fruit tree producers using ordered probit models. Tart considered productivity firmness most...

Cultivation of yams by rural households contributes to food supply and income generation. Notwithstanding the importance this crop, yam production has been stagnating, threatening livelihoods security. Contrary traditional systems in Ghana, an alternative system that integrates pigeonpea is being proposed mitigate constraints existing through replenishing soil fertility, provision stakes for staking, providing nutritious grains farmers. The purpose study was explore farmers’ determine...

Abstract The wood adulteration is a common problem and under-studied aspect in the timber industry of Sri Lanka. Hence we conducted survey to assess status check applicability morphometric parameters DNA barcoding detect adulterated sources. We interviewed stakeholders collect information regarding adulterations. measured parameters; density sizes xylem elements standard adulterant species. For barcoding, was extracted from selected species subjected PCR using markers, matK - trnT atpB rbcL...

The complete genome sequences of apple, peach, and diploid strawberry - one member each the three main fruit-producing branches Rosaceae tree were available in 2010. Despite this achievement, virtually none genomics knowledge was being used to assist breeding efforts these crops. Four years later, gap has been bridged, with genetic information routinely many US cherry programs. For example, DNA tests predict apple crispness, peach maturity date, fruit size, enabling breeders determine best...

Yam production along the West African yam belt is challenged with deteriorating soil fertility and unavailability of stakes, resulting in decreased productivity, farmers' livelihood. This study evaluated resource use productivity pigeonpea-yam cropping systems Ghana's forest forest-savannah transition zones from 2017 through 2019 seasons. Pigeonpea was established either an alley or as a border during season, while cultivated 2018 2019. A split-plot design system (yam planted alleys...

BACKGROUND: Understanding the genetics of flowering in strawberry (Fragaria × ananassa) will aid development breeding strategies.OBJECTIVE: To search for quantitative trait loci (QTL) associated with remontancy and weeks strawberry.METHODS: Previously collected phenotypic data from two non-remontant 'Honeoye' remontant 'Tribute' populations simple sequence repeats (SSR) markers were used to QTL repeat flowering, runner production, as well ability produce flowers runners at 17, 20 23 • C....

Bean rust is a devastating disease of snap beans in Sri Lanka. Our study endeavored to develop with resistance Uromyces appendiculatus, the bean pathogen reported have numerous virulent races. Accordingly, we needed detailed understanding virulence U. appendiculatus and identify genes conferring effective resistance. To investigate virulence, collected leaves symptoms from four producing districts From these samples established 57 single pustule isolates. Each isolate was inoculated on set...

Consumers purchase fresh strawberries all year long. Extending the fruiting season for new strawberry cultivars is a common breeding goal. Understanding inheritance of repeat key to improving efficiency. Several independent research groups using multiple genotypes and analytic approaches have identified single genomic region in associated with fruiting. Markers mapped this were used evaluate parents from United States Department Agriculture - Agricultural Research Service (USDA-ARS) program...

Rice blast, is a serious disease affecting rice, caused by fungal pathogen Magnaporthe grisea (anarmorph - Pyricularia grisea). This paper summarizes the progress and achievements made in detection of blast resistance genes phenotypically evaluated, selected rice cultivars Sri Lanka based on simple sequence repeats (SSR) DNA markers. In present study was identified morphological characters, molecular techniques which confirmed identity as M. grisea. Total 34 varieties including several...

Abstract The genetic diversity of the FMD viruses collected from outbreaks during second half 20 th Century in Sri Lanka was assessed present study. We sequenced VP1 genomic region samples FMDV epidemics caused by serotype O 1962 and 1997. For comparison, we related viral isolates other Asian countries. analyzed sequences strains using UPGMA method with uncorrected pairwise distances. Nucleotide divergence (ND) thresholds 15%–20% 5%–<15% were used to differentiate topotypes lineages,...

done in California using parents carrying day-neutral gene(s) from a Wasatch Mountain genotype of F. virginiana Mill. subsp. glauca (S. Watson) Staudt. The studies Powers (1954) and Hancock et al. (2001) were Wyoming Michigan, respectively, that carried genes ·ananassa wild clones different the source. study Ourecky Slate (1967) was performed NewYorkusingparentalmaterialthatdidnot contain any recently incorporated germplasm.

Foot-and-mouth disease (FMD) affects the livestock industry in a transboundary manner. It is essential to understand FMD phylodynamics assist disease-eradication. critically Sri Lankan cattle causing substantial economic losses. Even though many studies have covered serotyping and genotyping of virus (FMDV) Lanka, there significant knowledge gap exists understanding FMDV country. In present study, VP1 genomic region viral isolates belonging serotype C from Lanka other South Asian countries...

Phyllanthus emblica L.(V. Nelli) is an important constituent of indigenous medicine and a commercially fresh fruit species. The genomic diversity the P. germplasm in Sri Lanka has not been studied detail. Therefore, present study was conducted to molecularly characterize Lanka. Young tender leaves from 66 trees were collected various parts country for DNA assessment. Genomic extracted using DNeasy Plant mini kit. Six microsatellite (SSR) markers one SCAR marker developed used PCR...

The primary goal of this research was to evaluate the relative importance strawberry fruit quality and plant traits producers. Previous studies focus on that impact postharvest marketable yield; however, emphasizing these producers are scarce. To investigate U.S. producer trait preferences, a series audience surveys were conducted at four meetings across United States. Results indicate firmness, flavor, shelf life retail most important fruit/plant for successful cultivar possess. Growing...