Unblemished fully ripe fruit from five day-neutral strawberry cultivars were harvested on two separate dates and evaluated for ascorbic acid (AsA), sugars, phenolic composition. Individual phenolics determined by HPLC, total Folin–Ciocalteu (F–C) a 'new' assay: Fast Blue BB (FBBB), which detects directly. FBBB reported an average 2.9-fold greater concentration of than F–C, had significant correlation (r = 0.80; P 0.001) with via HPLC did not interact AsA or whereas indirect detection assay...

Although simple sequence repeat (SSR) markers have been developed for species in the closely related genera Fragaria L. (strawberry) and Rubus (raspberry blackberry), number of SSRs available is insufficient genetic mapping. Our objective was to use compare multiple approaches developing additional . The included: development from GenBank sequences varied relatedness identified with two different data-mining methods (BLAST SSRIT); evaluation some previously published designed species; a...

Abstract A linkage mapping approach was used to identify quantitative trait loci (QTL) associated with day‐neutrality in the commercial strawberry, Fragaria × ananassa (Duch ex Rozier). Amplified Fragment Length Polymorphism (AFLP) markers were build a genetic map population of 127 lines developed by crossing day‐neutral (DN) ‘Tribute’ short‐day (SD) ‘Honeoye’. The genotyped AFLP and 429 single dose restriction fragments (SDRF) placed on consensus 1541 cM 43 groups. Individuals from observed...

Recent evolution of separate sexes in flowering plants provides unparalleled opportunities for understanding the early stages sex chromosome evolution, including their origin from autosomes. Moreover, transition combined to can be associated with speciation via polyploidization angiosperms, suggesting that genome doubling/merger may facilitate sterility mutations required formation. To gain insight into chromosomes a polyploid plant, we doubled simple sequence repeat (SSR) density and...

Strawberry branch crown development is often associated with yield and berry size. The propagation rate of strawberry cultivars key to their establishment longevity in the industry. However, new are released little indication these traits. This study aimed characterize according production crowns, runners, daughter plants, determine if cultivar plant part would vary system, plants between field environments tubs a screenhouse, how well subjective scores for vigor runner predict objective...

Fruit of the cultivated strawberry ( Fragaria × ananassa Duchesne ex Rozier) are a good source natural antioxidants, which play an important role in protecting human health. Antioxidant levels vary considerably among genotypes. The is hybrid two very different wild progenitor species: F. virginiana Mill. and chiloensis (L.) species valued by breeders as sources novel traits, but have not been evaluated for antioxidant capacity or compounds. objectives this study 1) to evaluate contents...

The non-climacteric octoploid strawberry (Fragaria × ananassa Duchesne ex Rozier) was used as a model to study its regulation during fruit ripening. High performance liquid chromatography electrospray tandem-mass spectrometry (HPLC-ESI-MS/MS) employed profile 28 different endogenous phytohormones in strawberry. These include auxins, cytokinins (CKs), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic (ACC), jasmonates, and phenolic compounds salicylic (SA), benzoic...

The rise of sexual dimorphism is thought to coincide with the evolution sex chromosomes. Yet because chromosomes in many species are ancient, we lack empirical evidence earliest stages this transition. We use QTL analysis examine genetic architecture subdioecious octoploid Fragaria virginiana. demonstrate that region housing male‐function locus controls majority quantitative variation proportion fruit set, confirming existence a proto‐sex chromosome, and houses major for eight additional...

Strawberries are an economically important crop partly because they very popular with consumers. Strawberry growers and breeders would benefit from knowing which cultivars fruit traits consumers prefer. The objectives of this study were to (1) determine consumer preference for the once-fruiting repeat-fruiting strawberry available grow in Mid-Atlantic help select most suitable based on their marketability, critical sensory quality attributes, physicochemical measurements; (2) identify...

Interest in molecular markers and genetic maps is growing among researchers developing new cultivars of Rubus L. (raspberry blackberry). Several traits interest fail to express seedlings or reliably some environments are candidates for marker-assisted selection. A number simple sequence repeat (SSR) derived from Fragaria (strawberry) available use with mapping populations. The objectives this study were test 142 these SSR screen raspberry blackberry parental genotypes potential existing...

The recent development of novel repeat-fruiting types blackberry (Rubus L.) cultivars, combined with a long history morphological marker-assisted selection for thornlessness by breeders, has given rise to increased interest in using molecular markers facilitate breeding. Yet no genetic maps, markers, or even sequences exist specifically cultivated blackberry. purpose this study is begin these tools generating and annotating the first expressed sequence tag (EST) library, designing primers...

Strawberries (Fragaria ×ananassa Duchesne ex Rozier) are economically valuable and popular with consumers who want to buy them all year round. However, a typical harvest season in most U.S. states is limited only 3 4 weeks. Repeat-fruiting cultivars grown these could help meet that demand, especially if bred be adapted those states. To develop new repeat-fruiting modern standards when the Mid-Atlantic similar regions, production system utilizing low tunnels over raised white-plastic-covered...

Abstract We present 37 microsatellite primer pairs developed from a cDNA library of Fragaria x ananassa Duch. cv. Strawberry Festival. Polymorphism was high and the number presumptive alleles 13 expressed sequence tag–simple repeats (EST–SSRs) in 70 strawberry cultivars ranged five to 32 per pairs, averaging 16.1. Cross‐species amplification also 89% vesca L. 100% progenitor species octoploid strawberry, chiloensis (L.) virginiana

Improved methods to produce hybrid soybean [ Glycine max (L.) Merr.] seed could augment several types of research. Two previously described methods, the traditional method and dilution method, require insect‐facilitated cross‐pollination ms nuclear male‐sterile plants. The requires a substantial time investment during flowering remove fertile siblings, amount land pollen‐parent seed. Because time, land, are limited, more efficient would be valuable. cosegregation was developed, utilizing...

The cultivated strawberry, Fragaria × ananassa Duchesne ex Rozier, originated via hybridization between octoploids F. chiloensis (L.) Mill. and virginiana These three octoploid species are thought to share a putative genome composition of AAA`A'BBB`B'. Diploid vesca L., is considered have donated the A genome. Current attention development diploid model system for strawberry genomics warrants assessment simple sequence repeat (SSR) marker transferability in L. In present study, 23 SSR primer...