A5070556375- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Plant responses to water stress
- Soybean genetics and cultivation
- Biochemical and Molecular Research
- Plant Reproductive Biology
- Nematode management and characterization studies
- Plant Pathogens and Fungal Diseases
- Genomics and Chromatin Dynamics
- Lymphoma Diagnosis and Treatment
- Nuclear Structure and Function
- Peroxisome Proliferator-Activated Receptors
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Pancreatic function and diabetes
- RNA Research and Splicing
- Ginseng Biological Effects and Applications
- Legume Nitrogen Fixing Symbiosis
- Mast cells and histamine
- Adipose Tissue and Metabolism
Beijing YouAn Hospital
2022-2025
Capital Medical University
2017-2025
Agricultural Research Service
2006-2024
China Medical University
2020
Beltsville Agricultural Research Center
2006-2018
United States Department of Agriculture
2009-2016
Pioneer Hi-Bred
2006
University of New Hampshire
2006
University of Massachusetts Amherst
1997-1998
Abstract We used the Exploris 240 mass spectrometer for non-targeted metabolomics on Saccharomyces cerevisiae strain BY4741 and tested AcquireX software increasing number of detectable compounds Compound Discoverer 3.3 identifying by MS 2 spectral library matching. increased potentially identifiable 50% through six iterations acquisition. On basis high-scoring matches made Discoverer, there were 483 putatively identified from nearly 8000 candidate spectra. Comparisons to 20 amino acid...
Abstract The methyltransferase like 3 (METTL3) has been generally recognized as a nuclear protein bearing oncogenic properties. We find predominantly cytoplasmic METTL3 expression inversely correlates with node metastasis in human cancers. It remains unclear if is functionally distinct from cytosolic driving tumorigenesis and, any, how tumor cells sense insults to coordinate functions within these intracellular compartments. Here, we report an acetylation-dependent regulation of localization...
Protein post-translational modifications (PTMs) are crucial for cancer cells to adapt hypoxia; however, the functional significance of lysine crotonylation (Kcr) in hypoxia remains unclear. Herein we report a quantitative proteomics analysis global crotonylome under normoxia and hypoxia, demonstrate 128 Kcr site alterations across 101 proteins MDA-MB231 cells. Specifically, observe significant decrease K131cr, K156cr K220cr phosphoglycerate kinase 1 (PGK1) upon hypoxia. Enoyl-CoA hydratase...
Abstract An enhanced NADH/NAD + ratio, termed reductive stress, is associated with many diseases. However, whether a downstream sensing pathway exists to mediate pathogenic outcomes remains unclear. Here, we generate soluble pyridine nucleotide transhydrogenase from Escherichia coli ( Ec STH), which can elevate the ratio and meantime reduce NADPH/NADP ratio. Additionally, fuse STH previously described Lb NOX (a water-forming NADH oxidase Lactobacillus brevis ) resume With these tools by...
The stimulatory and inhibitory role of ethylene auxin, respectively, in leaf abscission (leaf drop) is well documented. More recently, IDA (INFLORESCENCE DEFICIENT IN ABSCISSION) peptides their putative interacting receptor-like-kinase partners, HAESA HAESA-like2, were shown to be essential components Arabidopsis floral organ abscission. Prior research on IDA, it was reported that bean (Phaseolus vulgaris) required a diffusible signal emanated from the vascular tissue. We interested...
INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) is a signaling peptide that regulates cell separation in Arabidopsis including floral organ abscission and lateral root emergence. IDA highly conserved dicotyledonous flowering plant genomes. IDA-like sequences were also found the genomic of root-knot nematodes, Meloidogyne spp., which are globally deleterious pathogens agriculturally important plants, but role these genes unknown. Exogenous treatment ida mutant with synthetic identical to M....
Tissue culture-generated shoot-based clonal lines are being used to investigate the role of proline-linked pentose phosphate pathway in stimulating rosmarinic acid (RA). In this study is reported stimulation RA biosynthesis oregano line O-1 response proline, proline precursors (ornithine and arginine), analogue (azetidine-2-carboxylate, A2C). Following exogenous treatment with presence or absence A2C, significantly enhanced content concurrently higher levels endogenous were observed compared...
Abscission zone (AZ) development and the progression of abscission (detachment plant organs) have been roughly separated into four stages: first, AZ differentiation; second, competence to respond signals; third, activation abscission; fourth, formation a protective layer post-abscission trans-differentiation. Stage three, abscission, is when changes in cell wall extracellular matrix occur support successful organ separation. Most research has focused on gene expression for enzymes that...
Fatty acid uptake is essential for cell physiological function, but detailed mechanisms remain unclear. Here, we generated an acetyl-CoA carboxylases (ACC1/2) double-knockout line, which lacked fatty biosynthesis and survived on serum acids was used to screen inhibitors. We identified a Food Drug Administration–approved tricyclic antidepressant, nortriptyline, that potently blocked both in vitro vivo. also characterized underlying whereby nortriptyline provoked lysosomes release protons...
Abscission, organ separation, is a developmental process that modulated by endogenous and environmental factors. To better understand the molecular events underlying progression of abscission in soybean, an agriculturally important legume, we performed RNA sequencing (RNA-seq) isolated from leaf zones (LAZ) petioles (Non-AZ, NAZ) after treating stem/petiole explants with ethylene for 0, 12, 24, 48, 72 h. As expected, expression several families cell wall modifying enzymes many...
Colonization of plant roots by root knot and cyst nematodes requires a functional ethylene response pathway.However, plays many roles in development whether its role nematode colonization is direct or indirect, for example lateral initiation hair growth, not known.The temporal requirement localized synthesis during the life span soybean (SCN) on was further investigated.Although significant increase evolution detected from SCN-colonized roots, concentration immediate precursor to ethylene,...
Epithelial-to-mesenchymal transition (EMT) activation is important in cancer progression and metastasis. Evidence indicates that nc886 a representative Pol III gene processes microRNA products via Dicer further downregulates its target transforming growth factor- β1 (TGF-β1), which the most prominent inducer of EMT prostate (PC). Consistent with previous literature, we found downregulation was strongly associated metastatic behavior showed worse outcomes PC patients. However, little known...
The cultivated strawberry, Fragaria × ananassa Duchesne ex Rozier, originated via hybridization between octoploids F. chiloensis (L.) Mill. and virginiana These three octoploid species are thought to share a putative genome composition of AAA`A'BBB`B'. Diploid vesca L., is considered have donated the A genome. Current attention development diploid model system for strawberry genomics warrants assessment simple sequence repeat (SSR) marker transferability in L. In present study, 23 SSR primer...
Sulforaphane (SFN) was demonstrated to induce apoptosis in a variety of cancers via multiple mechanisms. However, owing short half-life circulation, SFN not used for clinical treatment yet. Interestingly, analog, sulforaphane-cysteine (SFN-Cys) has longer metabolism, and we previously that SFN-Cys inhibited invasion human prostate cancer cells. Here, would investigate whether induces find the underlying mechanisms non-small cell lung (NSCLC) Western blots were test molecular linkages among...
The EGFR-RAS-ERK pathway plays a key role in cancer development and progression. However, the integral assembly of signaling complexes from upstream component EGFR to downstream ERK is largely unknown. Here, we show that hematopoietic PBX-interacting protein (HPIP) interacts with all classical components forms at least two overlapping components. Experiments HPIP knockout or knockdown chemical inhibition expression showed required for complex formation, activation, signaling–mediated...
Abstract Natural phenolic antioxidants from genetically heterogeneous plants are becoming important for prevention of lipid oxidation in food. To produce uniform and enhanced levels we have developed elite clonal lines rosemary using plant tissue culture techniques. A cytokinin (benzyladenine)‐induced adventitious shoot multiplication was rosemary. Optimum occurred Murashige Skoog medium containing 1 mg/1 benzyladenine 2 mM proline. About 3–10 shoots were induced each expiant originating a...
Abstract We hypothesized that soybean cyst nematode (SCN; Heterodera glycines) co-opts part or all of one more innate developmental process in (Glycine max) to establish its feeding structure, syncytium, roots. The syncytium is formed within the vascular bundle by partial degradation cell walls and membranes between adjacent parenchyma cells. A mature incorporates as many 200 cells into large multinucleated cell. Gene expression patterns for several wall-modifying proteins were compared...
Abstract The major cause of mortality for prostate cancer (PCa) is metastasis; however, the metastatic mechanism remains unclear. MicroRNAs (miRNAs) alter expression patterns essential genes through posttranscriptional regulation during development. study was mainly aimed at identifying specific miRNA‐messenger RNA (mRNA) interactions and signaling pathways associated with PCa distant metastasis. New analytical approaches were applied, combining miRNA gene microarray, to screen...
Abstract Non‐coding RNA 886 (nc886/VTRNA2‐1) is a Pol III transcript and an atypical imprinted gene. Its exact function as negative regulator of protein kinase R establishes its connection with innate immunity. Studies have shown that nc886 silencing closely associated prostate cancer progression. Previous work has constructed cell model stable overexpression (“mimic” or “nc886 + ”) in PC‐3M‐1E8 lines (1E8), which are highly bone‐metastatic human cells low expression nc886, expressing the...