A5083760635- Genomics and Chromatin Dynamics
- Plant Pathogens and Fungal Diseases
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Powdery Mildew Fungal Diseases
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- FOXO transcription factor regulation
- Wheat and Barley Genetics and Pathology
- Protein Degradation and Inhibitors
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Plant responses to elevated CO2
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
RWTH Aachen University
2018-2024
Fraunhofer Institute for Molecular Biology and Applied Ecology
2018
Increasing the productivity of crops is a major challenge in agricultural research. Given that photosynthetic carbon assimilation necessary for plant growth, enhancing efficiency photosynthesis one strategy to boost productivity. The authors attempted increase and biomass tobacco plants by expressing individual components Chlamydomonas reinhardtii concentration mechanism (CCM) integrating them into chloroplast. Independent transgenic varieties are generated accumulating carbonic anhydrase...
Rust and powdery mildew fungi are widespread obligate biotrophic phytopathogens. They colonize a broad range monocotyledonous dicotyledonous host plant species in the case of crop plants can cause severe yield losses. While mildews (Ascomycota) grow mainly epiphytically infect epidermis, rust (Basidiomycota) typically enter tissues through stomata spread within intercellular spaces. Both fungal taxa have unusually large genomes that rich repetitive elements (mostly derived from...
Powdery mildew fungi are widespread and agronomically relevant phytopathogens causing major yield losses. Their genomes have disproportionately large numbers of mobile genetic elements, they experienced a significant loss highly conserved fungal genes. In order to learn more about the evolutionary history this group, we explored genome an Asian oak tree pathogen, Parauncinula polyspora , species that diverged early during evolution from remaining powdery fungi. We found P. draft is...
Abstract Changes in gene expression programs are intimately linked to cell fate decisions. Post-translational modifications of core histones contribute control expression. Methylation lysine 4 histone H3 (H3K4) correlates with active promoters and transcription. This modification is catalyzed by KMT2 methyltransferases, which require interaction subunits, WDR5, RBBP5, ASH2L DPY30, for catalytic activity. Ash2l necessary organismal development tissue homeostasis. In mouse embryo fibroblasts...
Powdery mildews are obligate biotrophic fungal pathogens causing important diseases of plants worldwide. Very little is known about the requirements for their pathogenicity at molecular level. This largely due to inability culture these organisms in vitro or modify them genetically. Here, we describe a mutagenesis procedure based on ultraviolet (UV) irradiation accumulate mutations haploid genome barley powdery mildew pathogen Blumeria graminis f. sp. hordei. Exposure B. hordei conidia...
Summary Hosts and pathogens typically engage in a coevolutionary arms race. This also applies to phytopathogenic powdery mildew fungi, which can rapidly overcome plant resistance perform host jumps. Using experimental evolution, we show that the pathogen Blumeria hordei is capable of breaking agriculturally important broad‐spectrum conditioned by barley loss‐of‐function mlo mutants. Partial virulence evolved B. isolates correlated with distinctive pattern adaptive mutations, including...
Abstract Gene expression is controlled in part by post-translational modifications of core histones. Methylation lysine 4 histone H3 (H3K4), associated with open chromatin and gene transcription, catalyzed type 2 methyltransferase complexes that require WDR5, RBBP5, ASH2L DPY30 as subunits. Ash2l essential during embryogenesis for maintaining adult tissues. To expand on the mechanistic understanding Ash2l, we generated mouse embryo fibroblasts (MEFs) conditional alleles. Upon loss...
Abstract The trithorax protein ASH2L is essential for organismal and tissue development. As a subunit of COMPASS/KMT2 complexes, necessary methylation histone H3 lysine 4 (H3K4). Mono- tri-methylation at this site mark active enhancers promoters, respectively, although the functional relevance H3K4 only partially understood. has long half-life, which results in slow decrease upon knockout. This made it difficult to define direct consequences. To overcome limitation, we employed PROTAC system...
Due to their comparatively small genome size and short generation time, fungi are exquisite model systems study eukaryotic evolution. Powdery mildew (PM) present an exceptional case where strict host dependency (a lifestyle termed obligate biotrophy) is associated with some of the largest fungal genomes sequenced so far (>100 Mbp). This expansion largely due pervasiveness transposable elements (TEs), which can cover more than 70% these genomes, loss multiple conserved ascomycete genes...
Hosts and pathogens typically engage in a co-evolutionary arms race. This also applies to phytopathogenic powdery mildew fungi, which can rapidly overcome plant resistance perform host jumps. Using experimental evolution, we show that the pathogen Blumeria hordei is capable of breaking agriculturally important broad-spectrum conditioned by barley loss-of-function mlo mutants. Partial virulence evolved B. isolates correlated with distinctive pattern adaptive mutations, including small-sized...
Abstract The trithorax protein ASH2L is essential for organismal and tissue development. As a subunit of COMPASS/KMT2 complexes, necessary methylation histone H3 lysine 4 (H3K4). Mono- trimethylation at this site mark active enhancers promoters, respectively, although the molecular relevance H3K4 only partially understood. Due to importance in all 6 COMPASS-like complexes its long half-life, it has been difficult define direct consequences. To overcome limitation, we employed PROTAC system,...
ABSTRACT Gene expression is controlled in part by post-translational modifications of core histones. Methylation lysine 4 histone H3 (H3K4), associated with open chromatin and gene transcription, catalyzed type 2 methyltransferase complexes that require WDR5, RBBP5, ASH2L DPY30 as subunits. Ash2l essential during embryogenesis for maintaining adult tissues. To expand on the mechanistic understanding Ash2l, we generated mouse embryo fibroblasts (MEFs) conditional alleles. Upon loss...
Abstract Changes in gene expression programs are intimately linked to cell fate decisions. Post-translational modifications of core histones contribute control expression. Methylation lysine 4 histone H3 (H3K4) correlates with active promoters and transcription. This modification is catalyzed by KMT2 methyltransferases, which require interaction subunits, WDR5, RBBP5, ASH2L DPY30, for catalytic activity. Ash2l necessary organismal development tissue homeostasis. In mouse embryo fibroblasts...