A5101438175- Enzyme Structure and Function
- Vitamin D Research Studies
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Estrogen and related hormone effects
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Hemoglobin structure and function
- Amino Acid Enzymes and Metabolism
- Biotin and Related Studies
- Peptidase Inhibition and Analysis
- Microplastics and Plastic Pollution
- T-cell and B-cell Immunology
- Retinoids in leukemia and cellular processes
- Carcinogens and Genotoxicity Assessment
- Porphyrin Metabolism and Disorders
- Pharmacological Effects and Toxicity Studies
- Advanced Thermodynamics and Statistical Mechanics
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Chemical Synthesis and Analysis
- RNA modifications and cancer
- Advanced NMR Techniques and Applications
- Carbohydrate Chemistry and Synthesis
Western Digital (Japan)
2025
Tokyo Medical and Dental University
2015-2024
The University of Tokyo
2002-2023
The Open University of Japan
2015
Kyoto University
2015
Hokkaido University
2015
MED Institute
2013-2014
State Scientific Center of the Russian Federation - Federal Medical Biophysical Center named after A.I. Burnazyan
2013
Federal Medical-Biological Agency
2013
Tokyo Medical University
2011
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSegmental Isotope Labeling for Protein NMR Using Peptide SplicingToshio Yamazaki, Takanori Otomo, Natsuko Oda, Yoshimasa Kyogoku, Koichi Uegaki, Nobutoshi Ito, Yoshizumi Ishino, and Haruki NakamuraView Author Information Institute Research, Osaka University 3-2 Yamadaoka, Suita, 565-0871, Japan Biomolecular Engineering Research 6-2-3 Furuedai, 565-0874, National AIST 1-8-31 Midorigaoka, Ikeda, 563-8577, Cite this: J. Am. Chem. Soc. 1998, 120,...
Abstract Summary: The Protein Data Bank (PDB) has recently released versions of the PDB Exchange dictionary and archival data files in XML format collectively named PDBML. automated generation these is driven by infrastructure use at PDB. correspondences between schema metadata are described as well representations dictionaries files. Availability: current software translated file located http://deposit.pdb.org/pdbML/pdbx-v1.000.xsd, on mmCIF resource page http://deposit.pdb.org/mmcif/....
We report here the design and synthesis of a novel vitamin D receptor (VDR) agonist whose hydrophobic core structure is p-carborane (1,12-dicarba-closo-dodecaborane, an icosahedral carbon-containing boron cluster having remarkable thermal chemical stability characteristically B–H surface). This carborane-based VDR ligand exhibited moderate activity, comparable to that natural hormone, despite its simple flexible structure. X-ray analysis provided direct evidence carborane cage binds surface...
Abstract Autophosphorylation of amino-acid residues is part the folding process various protein kinases. Conventional chemical screening mature kinases has missed inhibitors that selectively interfere with process. Here we report a cell-based assay evaluates inhibition kinase at transitional state during and identify intermediate-selective inhibitor dual-specificity tyrosine-phosphorylation-regulated 1A (DYRK1A), which refer to as FINDY. FINDY suppresses intramolecular autophosphorylation...
A wide range of antiviral drugs is currently available; however, drug-resistant viruses have begun to emerge and represent a potential public health risk. Here, we explored the use compounds that inhibit or interfere with action essential host factors prevent virus replication. In particular, focused on cyclin-dependent kinase 9 (CDK9) inhibitor, FIT-039, which suppressed replication broad spectrum DNA through inhibition mRNA transcription. Specifically, FIT-039 inhibited herpes simplex 1...
A cutinase-type polyesterase from Saccharomonospora viridis AHK190 (Cut190) has been shown to degrade the inner block of polyethylene terephthalate. unique feature Cut190 is that its function and stability are regulated by Ca2+ binding. Our previous crystal structure analysis Cut190S226P showed one binds enzyme, which induces large conformational changes in several loop regions stabilize an open conformation [Miyakawa, T., et al. (2015) Appl. Microbiol. Biotechnol. 99, 4297]. In this study,...
Peptide segments in a protein, which can include an active site of interest or be series parts constituting the entire structure, are now selectively observed by nuclear magnetic resonance (NMR) spectroscopy using samples prepared intein-mediated ligation method. Two separate inteins were used to ligate NMR-transparent both ends NMR-visible segment, producing partly visible intact protein molecule. The 15N−1H correlation spectrum 370-residue maltose binding labeled with 15N at continuous...
SCA1 is a dominant spinocerebellar ataxia (SCA) and multi-systemic syndrome caused by abnormal expansion of unstable CAG repeat in novel gene located on chromosome 6p22–p23. We clinically studied 35 Japanese patients who were assumed to have come from common origin. The age at onset ranged 15–63 years, significantly correlated with units mutant alleles. Ataxia was the initial symptom, majority had similar history signs symptoms. Nystagmus first minimal, later attenuated, slow saccade...
In the present study, apoptotic, antioxidant and antiradical effects of majdine isomajdine from Vinca herbacea Waldst. Kit were studied. For testing possible apoptotic V. herbacea, DNA fragmentation assay was conducted on rat brain cortical tissue homogenates, in vitro. Also their mitochondrial activity tested by using same samples rats. addition, determined various vitro assays, including 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•+) radical scavenging...
Toll-like receptor 7 (TLR7) plays an essential role in development of systemic lupus erythematosus by co-stimulating B cells reactive to the endogenous TLR7 ligand Sm/ribonucleoprotein (RNP), a crucial self-antigen. However, how TLR7-mediated autoimmune response is regulated not yet known. In this study, we demonstrate that CD72, inhibitory cell co-receptor known prevent lupus, recognizes Sm/RNP at extracellular C-type lectin-like domain (CTLD) and specifically inhibits Sm/RNP. Moreover,...
In heat-assisted magnetic recording, field-driven magnetization switching of grains in media is assisted by heat provided laser diode head. A method called THMap was developed to measure the distribution grains. It measures noise power after reverse DC writing over saturated opposite polarity as a function current and write current. We investigated characteristics comparing with two-dimensional map bit error rate (BER) To remove impacts widened width on BER increased current, constant...
The X-ray crystal structures of the rat VDR ligand-binding domain complexed with 19-norvitamin D compounds that contain an adamantyl substituent at side-chain terminus, 2a (ADTT), 2b (ADNY), and 2c (ADMI4) a coactivator peptide derived from DRIP205 are reported. These show series partial agonistic (10-75% efficacy)/antagonistic activities. All these receptors crystallized in canonical active conformation, regardless their activity profiles. bulky side chain does not crowd helix 12 but...
To identify novel vitamin D receptor (VDR) ligands that induce a architecture within the ligand-binding pocket (LBP), we have investigated eight 22-butyl-1α,24-dihydroxyvitamin D3 derivatives (3−10), all having butyl group as branched alkyl side chain. We found 22S-butyl-20-epi-25,26,27-trinorvitamin derivative 5 was potent VDR agonist, whereas corresponding compound 4 with natural configuration at C(20) antagonist. Analogues full chain were less and whether they agonists or antagonists...
Excessive angiogenesis contributes to numerous diseases, including cancer and blinding retinopathy. Antibodies against vascular endothelial growth factor (VEGF) have been approved are widely used in clinical treatment. Our previous studies using SRPIN340, a small molecule inhibitor of SRPK1 (serine-arginine protein kinase 1), demonstrated that is potential target for the development antiangiogenic drugs. In this study, we solved structure bound SRPIN340 by X-ray crystallography. Using...
The energy landscape of a peptide [Ace-Lys-Gln-Cys-Arg-Glu-Arg-Ala-Nme] in explicit water was studied with multicanonical molecular dynamics simulation, and the AMBER parm96 force field used for calculation. taken from recognition helix DNA-binding protein, c-MYB: A rugged obtained, which random-coil conformations were dominant at room temperature. CD spectra synthesized revealed that it is random state However, 300 K canonical ensemble, Q(300K), contained alpha-helix, 3(10)-helix,...