A5101894394- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Skin Protection and Aging
- Autophagy in Disease and Therapy
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Cancer Research and Treatments
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Genomics, phytochemicals, and oxidative stress
- Melanoma and MAPK Pathways
- Hippo pathway signaling and YAP/TAZ
- Cell death mechanisms and regulation
- Light effects on plants
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Antioxidant Activity and Oxidative Stress
- 14-3-3 protein interactions
- PI3K/AKT/mTOR signaling in cancer
- Immunotherapy and Immune Responses
- Oral and Maxillofacial Pathology
- Liver physiology and pathology
- Calcium signaling and nucleotide metabolism
- Plant responses to water stress
- RNA modifications and cancer
KU Leuven
2005-2024
Institut Européen de Chimie et Biologie
2016
Université de Bordeaux
2016
Institut Polytechnique de Bordeaux
2016
Centre National de la Recherche Scientifique
2016
VIB-KU Leuven Center for Cancer Biology
2015
The University of Texas MD Anderson Cancer Center
2009-2014
Baylor College of Medicine
2011-2013
The University of Texas Health Science Center at Houston
2011
Autophagy and apoptosis are two important interconnected stress-response mechanisms. However, the molecular interplay between these pathways is not fully understood. To study fate function of autophagic proteins at onset apoptosis, we used a cellular model system in which autophagy precedes apoptosis. IL-3 depletion Ba/F3 cells caused caspase (casp)-mediated cleavage Beclin-1 PI3KC3, crucial components autophagy-inducing complex. We identified casp sites Beclin-1, TDVD(133) DQLD(149), yields...
Abstract Breast cancer research is hampered by difficulties in obtaining and studying primary human breast tissue, the lack of vivo preclinical models that reflect patient tumor biology accurately. To overcome these limitations, we propagated a cohort tumors grown epithelium-free mammary fat pad severe combined immunodeficient (SCID)/Beige nonobese diabetic (NOD)/SCID/IL-2γ-receptor null (NSG) mice under series transplant conditions. Both yielded stably transplantable xenografts at...
Abstract Transcriptional reprogramming of proliferative melanoma cells into a phenotypically distinct invasive cell subpopulation is critical event at the origin metastatic spreading. Here we generate transcriptome, open chromatin and histone modification maps cultures; integrate this data with existing transcriptome DNA methylation profiles from tumour biopsies to gain insight mechanisms underlying key event. This shows thousands genomic regulatory regions states, identifying SOX10/MITF...
We previously reported that dendritic cell-based mRNA vaccination plus ipilimumab (TriMixDC-MEL IPI) results in an encouraging rate of tumor responses patients with pretreated advanced melanoma. Here, we report the TriMixDC-MEL IPI-induced T-cell detected peripheral blood.Monocyte-derived cells electroporated encoding CD70, CD40 ligand, and constitutively active TLR4 (TriMix) as well tumor-associated antigens tyrosinase, gp100, MAGE-A3, or MAGE-C2 were administered together IPI for four...
Background Gastric cancer continues to be one of the deadliest cancers in world and therefore identification new drugs targeting this type is thus significant importance. The purpose study was identify validate a therapeutic agent which might improve outcomes for gastric patients future. Methodology/Principal Findings Using microarray technology, we generated gene expression profile human cancer–specific genes from tissue samples. We used Broad Institute's Connectivity Map analysis candidate...
Diagnosis of salivary gland neoplasms is challenging, especially on cytological specimens acquired by fine-needle aspiration. The recently implemented standardized Milan system for reporting cytopathology provides an estimated risk malignancy (ROM); yet, two the categories, diagnosis lesion remains unclear. However, a precise desirable optimal patient management, including planning surgery and imaging procedures.
Cutaneous squamous cell carcinoma (cSCC) is one of the most common cancers in Caucasian population. Although early stages skin cancer have a high curability and excellent prognosis, advanced cSCC shows resistance to chemotherapy, including cisplatin. The PI3-K/AKT pathway known role both development therapeutic drugs. In this study, we used isogenic lines representing different malignant transformation keratinocytes that were derived from dysplastic forehead (PM1), primary cutaneous SCC...
Mutational analysis guides therapeutic decision making in patients with advanced-stage gastrointestinal stromal tumors (GISTs). We evaluated three targeted next-generation sequencing (NGS) assays, consecutively used over 4 years our laboratory for mutational of 162 primary GISTs: Agilent GIST MASTR, Illumina TruSight 26 and an in-house developed 96 gene panels. In addition, we investigated the feasibility a more comprehensive approach by adding RNA (Archer FusionPlex, 11 genes) attempt to...
Autophagic cell death or abortive autophagy has been proposed to eliminate damaged as well cancer cells, but there remains a critical gap in our knowledge how this process is regulated. The goal of study was identify modulators the autophagic pathway and elucidate their effects on cellular signaling function. result siRNA library screenings show that an intact coatomer complex I (COPI) obligatory for productive autophagy. Depletion COPI members decreased survival impaired which preceded...
Autophagy, a programmed process in which cell contents are delivered to lysosomes for degradation, appears have both tumor-suppressive and tumor-promoting functions; stimulation inhibition of autophagy been reported induce cancer death, particular genes proteins associated positively negatively with autophagy. To provide basis incisive analysis those complexities ambiguities guide development new autophagy-targeted treatments cancer, we compiled comprehensive, curated inventory modulators by...
The identification of gene fusions has become an integral part soft tissue and bone tumour diagnosis. We investigated the added value targeted RNA-based sequencing (targeted RNA-seq, Archer FusionPlex) to our current molecular diagnostic workflow these tumours, which is based on fluorescence in situ hybridisation (FISH) for detection using 25 probes. In a series 131 samples RNA-seq identified fusion, BCOR internal tandem duplication or ALK deletion 47 cases (35.9%). For 74 cases,...
ABSTRACT Adequate photoprotection is essential to control UV‐related disorders, including sunburn, photoaging and photocarcinogenisis. Sun avoidance, protection of skin with clothing, sunscreens are presently the best way photoprotection, assuming that they used properly. However, new strategies, which based on or make use endogenous protective response UV light, may further improve currently photoprotective means. The addition repair enzymes and/or antioxidants has a positive effect skin's...