A5100353009- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Peptidase Inhibition and Analysis
- 3D Printing in Biomedical Research
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Cancer-related gene regulation
- Immune cells in cancer
- Neutropenia and Cancer Infections
- Immunotherapy and Immune Responses
- Machine Learning in Bioinformatics
- Cell Image Analysis Techniques
- Cancer Mechanisms and Therapy
- Advanced Biosensing Techniques and Applications
- Mesenchymal stem cell research
- Protease and Inhibitor Mechanisms
- Cytokine Signaling Pathways and Interactions
- Cancer Treatment and Pharmacology
- HIV/AIDS drug development and treatment
- Microtubule and mitosis dynamics
- Hematopoietic Stem Cell Transplantation
- Breast Cancer Treatment Studies
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
Nankai University
2025
The University of Texas MD Anderson Cancer Center
2013-2024
Network Group (Czechia)
2024
Affiliated Hospital of Nantong University
2024
Nantong University
2024
Beijing Proteome Research Center
2024
China Aerospace Science and Industry Corporation (China)
2024
Nanchang University
2023
First Affiliated Hospital of Nanchang University
2023
The First Affiliated Hospital, Sun Yat-sen University
2021-2022
Some breast cancers have been shown to contain a small fraction of cells characterized by CD44(+)/CD24(-/low) cell-surface antigen profile that high tumor-initiating potential. In addition, cancer propagated in vitro as mammospheres (MSs) also be enriched for capable self-renewal. this study, we defined gene expression signature common both and MS-forming cells. To examine its clinical significance, determined whether tumor surviving after conventional treatments were bearing...
We previously reported that extracellular histones are major mediators of death in sepsis. Infusion leads to increased cytokine levels. Histones activate TLR2 and TLR4 a process is enhanced by binding DNA. Activation responsible for the histone-dependent increase To study impact histone release on pathology we used two models: Con A-triggered activation T cells mimic sterile inflammation, acetaminophen model drug-induced tissue toxicity. were released both models anti-histone Abs protective....
Abstract Breast cancer research is hampered by difficulties in obtaining and studying primary human breast tissue, the lack of vivo preclinical models that reflect patient tumor biology accurately. To overcome these limitations, we propagated a cohort tumors grown epithelium-free mammary fat pad severe combined immunodeficient (SCID)/Beige nonobese diabetic (NOD)/SCID/IL-2γ-receptor null (NSG) mice under series transplant conditions. Both yielded stably transplantable xenografts at...
Resistance to neoadjuvant chemotherapy in triple-negative breast cancer can be mediated by a reversible chemotherapy-tolerant state.
Abstract Copper plays pivotal roles in metabolic homoeostasis, but its potential role human tumorigenesis is not well defined. Here, it revealed that copper activates the phosphoinositide 3‐kinase (PI3K)‐protein kinase B (PKB, also termed AKT) oncogenic signaling pathway to facilitate tumorigenesis. Mechanistically, binds 3‐phosphoinositide dependent protein 1 (PDK1), turn promotes PDK1 binding and subsequently downstream substrate AKT Blocking transporter (CTR1)‐copper axis by either...
Introduction Normal and malignant breast tissue contains a rare population of multi-potent cells with the capacity to self-renew, referred as stem cells, or tumor initiating (TIC). These can be enriched by growth “mammospheres” in three-dimensional cultures. Objective We tested hypothesis that human bone-marrow derived mesenchymal (MSC), which are known support metastasis, increase mammosphere formation. Results found MSC increased mammary epithelial cell (HMEC) formation dose-dependent...
Abstract Most triple negative breast cancers (TNBCs) are aggressively metastatic with a high degree of intra-tumoral heterogeneity (ITH), but how ITH contributes to metastasis is unclear. Here, clonal dynamics during were studied in vivo using two patient-derived xenograft (PDX) models established from the treatment-naive primary tumors TNBC patients diagnosed synchronous metastasis. Genomic sequencing and high-complexity barcode-mediated tracking reveal robust alterations architecture...
Background: The global mortality rate for coronavirus disease 2019 (COVID-19) is 3.68%, but the critically ill patients as high 50%. Therefore, exploration of prognostic predictors with COVID-19 vital prompt clinical intervention. Our study aims to explore predictive value hematological parameters in prognosis severe COVID-19. Methods: Ninety-eight who were diagnosed at Jingzhou Central Hospital and Wuhan, Hubei Province, included this study. Results: median age was 59 [28–80] years; a good...
Metastasis and disease relapse are hypothesized to result from tumor initiating cells (TICs). Previously, we have defined a CD44+/CD24−/low mammosphere-forming tumorigenic 493-gene signature in breast cancer. Stat3 was identified as critical node self-renewal based on an ongoing lentiviral shRNA screen being conducted two cancer cell lines SUM159 BT549. In corroborating work, targeting the SH2 domain of with novel small molecule decreased percentage expressing TIC markers (CD44+/CD24−/low...
Abstract Introduction Real-time monitoring of biologic changes in tumors may be possible by investigating the transitional cells such as circulating tumor (CTCs) and disseminated bone marrow (BM-DTCs). However, small numbers CTCs limited access to aspirates cancer patients pose major hurdles. The goal this study was determine whether breast (BC) patient-derived xenograft (PDX) mice could provide a constant renewable source BM-DTCs, thereby representing unique system for metastatic processes....
A flux dialysis method to measure unbound fraction (<i>f</i><sub>u</sub>) of compounds with high protein binding and other challenging properties was tested validated. This is based on the principle that initial rate a compound through size-excluding membrane proportional product concentration, <i>f</i><sub>u</sub>, permeability (<i>P</i><sub>mem</sub>). Therefore, <i>f</i><sub>u</sub> can be determined from concentration rate, assuming <i>P</i><sub>mem</sub> known. Compound rates for 14...
Abstract Physiologic roles of copper in metabolic homeostasis have been well established; however, whether and how is dysregulated tumors contributes to tumorigenesis not recapitulated. Here, we comprehensively summarize the potential origins accumulation diseases, especially cancers, by dysregulating transporter 1 (CTR1) or ATPase transporting alpha/beta (ATP7A/B) further demonstrate underlying mechanism contributing tumorigenesis. Specifically, addition modulating reactive oxygen species...
The overutilization of antibiotics in very preterm infants (VPIs) at low risk early-onset sepsis (EOS) is associated with increased mortality and morbidities. Nevertheless, the association early antibiotic exposure bronchopulmonary dysplasia (BPD) remains equivocal.
Hematogenous metastasis requires tumor cells to detach from primary into blood/lymphatic circulation and extravasate.Tumor in the blood system, named circulating (CTCs), are a suspension state, with unique cytoskeletal structure molecular phenotype different cells.The aim of this study is assess impact state on metastatic potential breast cancer (BCCs) its underlying mechanism.Methods: BCCs were cultured low-adhesion plates mimic state.Conventional adherent culture used as control.This...
Abstract Conditional overexpression of histone reader Tri partite m otif containing protein 24 (TRIM24) in mouse mammary epithelia ( Trim24 COE ) drives spontaneous development carcinosarcoma tumors, lacking ER, PR and HER2. Human carcinosarcomas or metaplastic breast cancers (MpBC) are a rare, chemorefractory subclass triple-negative (TNBC). Comparison morphology, TRIM24 levels derived gene signature reveals strong correlation with human MpBC tumors patient-derived xenograft (PDX) models....
Patient-derived xenograft (PDX) models of human breast cancer are proving useful for preclinical evaluation experimental therapeutics. However, until recently, generation PDX reflecting the full spectrum cancers has been an elusive goal. We recently developed a method establishing serially transplantable, phenotypically stable, in immunocompromised mice with comparatively high efficiency (overall ∼25%). These xenografts represent major clinically defined subtypes [e.g. estrogen receptor...
Abstract The response rate of anti-PD1 therapy is limited, and the influence on cancer patients unclear. To address these challenges, we conducted a longitudinal analysis plasma proteomic changes with in non-small cell lung (NSCLC), alveolar soft part sarcoma (ASPS), lymphoma patients. We included 339 samples before after from 193 NSCLC, ASPS, or lymphoma. proteins were detected using data-independent acquisition-mass spectrometry customable antibody microarrays. Differential characteristics...
Abstract Triple negative breast cancer (TNBC) accounts for 15–20% of cases in the United States. Systemic neoadjuvant chemotherapy (NACT), with or without immunotherapy, is current standard care patients early-stage TNBC. However, up to 70% TNBC have significant residual disease once NACT completed, which associated a high risk developing recurrence within two three years surgical resection. To identify targetable vulnerabilities chemoresistant TNBC, we generated longitudinal patient-derived...