A5007948264- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
- Biotin and Related Studies
- Luminescence and Fluorescent Materials
- Advanced Electron Microscopy Techniques and Applications
- Neurogenetic and Muscular Disorders Research
- Autism Spectrum Disorder Research
- Photodynamic Therapy Research Studies
- Congenital Anomalies and Fetal Surgery
- Protein Degradation and Inhibitors
- Amino Acid Enzymes and Metabolism
- Spectroscopy Techniques in Biomedical and Chemical Research
- Polydiacetylene-based materials and applications
- Dermatology and Skin Diseases
- RNA regulation and disease
- HIV-related health complications and treatments
- Cerebral Palsy and Movement Disorders
- Herpesvirus Infections and Treatments
- Optical Coherence Tomography Applications
- Congenital heart defects research
- Metabolism and Genetic Disorders
- Hereditary Neurological Disorders
- Genomic variations and chromosomal abnormalities
- Infant Development and Preterm Care
Chinese Academy of Sciences
2023-2025
Dalian Institute of Chemical Physics
2023-2025
University of Chinese Academy of Sciences
2023-2025
Guangxi Maternal and Child Health Hospital
2011-2024
State Key Laboratory of Applied Organic Chemistry
2020
Lanzhou City University
2020
Lanzhou University
2020
China Nonferrous Metal Mining (China)
2020
Boston Children's Hospital
2015
Single-molecule localization super-resolution fluorescence imaging relies on the ON/OFF switching of fluorescent probes to break diffraction limit. However, unreacted or nonspecifically bound cause non-targeted switching, resulting in substantial background that significantly reduces precision and accuracy. Here, we report a blinkogenic probe HM-DS655-Halo remains blinking OFF until it binds HaloTag, thereby triggering its self-blinking activity enabling application direct SMLM living cells...
Abstract Lysosomal morphology and pH dynamics are key indicators of lysosomal function, making long‐term single‐molecule localization microscopy (SMLM) imaging a promising tool for functional diagnostics. However, phototoxicity often compromises reliability. Here, we develop Aze‐HMSiR , spontaneously blinking silicon rhodamine probe with near‐infrared excitation, enabling low‐phototoxicity, (50 min) SMLM dynamics. This provides super‐resolution insights into distribution, size, lumen pH,...
Lysosomal morphology and pH dynamics are closely linked to lysosome functions, making long‐term single‐molecule localization microscopy (SMLM) imaging of whole‐cell lysosomes a potential indicator for functional diagnostics. However, the phototoxicity probes in SMLM often compromises reliability observed lysosomal dynamics. Here, we developed Aze‐HMSiR, spontaneously blinking silicon rhodamine probe with near‐infrared excitation, enabling low phototoxicity, morphology, up 50 minutes. This...
Fluorescence super-resolution microscopy has enabled nanoscale imaging of intracellular structures, but it remains challenging to simultaneously achieve structural and quantitative functional characterization, such as pH measurement, within the same region. Here, we introduce Two-Color Single-Molecule Blinking Ratiometricity (2C-SMBR), a novel method that integrates with single-molecule precision. By loading lysosomes two pH-dependent spontaneously blinking fluorophores distinct colors,...
Fluorescence super‐resolution microscopy has enabled nanoscale imaging of intracellular structures, but it remains challenging to simultaneously achieve structural and quantitative functional characterization, such as pH measurement, within the same region. Here, we introduce Two‐Color Single‐Molecule Blinking Ratiometricity (2C‐SMBR), a novel method that integrates with single‐molecule precision. By loading lysosomes two pH‐dependent spontaneously blinking fluorophores distinct colors,...
Photodynamic inactivation (PDI) has emerged as a promising antibacterial strategy that mitigates the risk of bacterial resistance. However, precise morphological dynamics and mechanisms underlying cell death during PDI...
Excited-state intramolecular proton transfer (ESIPT) fluorophores are valuable for ratiometric bioimaging due to their microenvironmental sensitivity, but traditional enol-keto systems suffer from poor biocompatibility and reduced efficiency in polar, protic environments. Here, we introduce a tail-assisted ESIPT (ta-ESIPT) strategy which occurs an amide donor amino nitrogen acceptor. This mechanism applies biocompatible charge-transfer fluorophores, such as naphthalimide, coumarin, NBD,...
By incorporating hybrid FRET pairs with RFPs as energy donors and TMSiR the acceptor, intersystem crossing (ISC) of is suppressed via FRET, leading to enhanced photostability RFPs.
Background Spinal muscular atrophy (SMA) is a genetic progressive neuromuscular disease. Nusinersen the first disease modifying drug approved to treat patients with SMA. Our study aimed evaluate efficacy of nusinersen treatment on motor function in children Methods A retrospective analysis was conducted data 52 genetically confirmed SMA from November 2020 September 2023. Motor assessed based standardized scales baseline 14 months follow-up. Results Of this study, majority had type 2 (40/52,...
A graphene oxide-aided triple helical aggregation-induced emission biosensor provides a highly specific sensing platform for charged collagen peptides.
Abstract MARK2 , a member of the evolutionarily conserved PAR1/MARK serine/threonine kinase family, has been identified as novel risk gene for autism spectrum disorder (ASD) based on enrichment de novo loss-of-function (Lof) variants in large-scale sequencing studies ASD individuals. However, features shared by affected individuals and molecular mechanism during early neural development remained unclear. Here, we report 31 carrying heterozygous presenting with ASD, other neurodevelopmental...
Abstract Single‐molecule localization super‐resolution fluorescence imaging relies on the ON/OFF switching of fluorescent probes to break diffraction limit. However, unreacted or nonspecifically bound cause non‐targeted switching, resulting in substantial background that significantly reduces precision and accuracy. Here, we report a blinkogenic probe HM‐DS655‐Halo remains blinking OFF until it binds HaloTag, thereby triggering its self‐blinking activity enabling application direct SMLM...
Christianson syndrome (CS) is an X-linked neurodevelopmental characterized by microcephaly, epilepsy, ataxia, and severe generalized developmental delay. Pathogenic mutations in the SLC9A6 gene, which encodes Na+/H+ exchanger protein member 6 (NHE6), are associated with CS autism spectrum disorder males. In this study, whole exome sequencing (WES) Sanger revealed a novel de novo frameshift variant c.1548_1549insT of 14-month-old boy early-onset seizures. According to The American College...
Objective To explore the physical development and immune function of infants without human immunodeficiency virus(HIV) infection who were delivered by HIV-infected mothers. Methods Two hundred ninety-seven mothers in Guangxi province from January 2008 to November 2011 selected as observation group.According whether had HIV or not, children further divided into HIV-infection group non-HIV according presence absence infection, antiretroviral drug (ART) treatment non-ART mother used ART...