A5038297029- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- Pineapple and bromelain studies
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Antimicrobial Resistance in Staphylococcus
- Calcium signaling and nucleotide metabolism
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Use and Resistance
- Galectins and Cancer Biology
Helmholtz Institute for Pharmaceutical Research Saarland
2019-2023
Helmholtz Centre for Infection Research
2019-2023
Saarland University
2019-2022
German Center for Infection Research
2019-2022
Chronic infections by Pseudomonas aeruginosa are characterized biofilm formation, which effectively enhances resistance toward antibiotics. Biofilm-specific antibiotic delivery could locally increase drug concentration to break antimicrobial and reduce the drug's peripheral side effects. Two extracellular P. lectins, LecA LecB, essential structural components for formation thus render a possible anchor biofilm-targeted delivery. The standard-of-care ciprofloxacin suffers from severe systemic...
Bacterial adhesion, biofilm formation and host cell invasion of the ESKAPE pathogen Pseudomonas aeruginosa require tetravalent lectins LecA LecB, which are therefore drug targets to fight these infections. Recently, we have reported highly potent divalent galactosides as specific inhibitors. However, they suffered from very low solubility an intrinsic chemical instability due two acylhydrazone motifs, precluded further biological evaluation. Here, isosterically substituted acylhydrazones...
The Gram-negative pathogen Pseudomonas aeruginosa causes severe infections mainly in immunocompromised or cystic fibrosis patients and is able to resist antimicrobial treatments. extracellular lectin LecB plays a key role bacterial adhesion the host biofilm formation. For inhibition of LecB, we designed synthesized set fucosyl amides, sulfonamides, thiourea derivatives. Then, analyzed their binding competitive direct assays. We identified β-fucosyl amides as unprecedented high-affinity...
Chronic Pseudomonas aeruginosa infections are characterized by biofilm formation, a major virulence factor of P. and cause extensive drug resistance. Fluoroquinolones effective antibiotics but linked to severe side effects. The two extracellular aeruginosa-specific lectins LecA LecB key structural components can be exploited for targeted delivery. In this work, several fluoroquinolones were conjugated lectin probes cleavable peptide linkers yield lectin-targeted prodrugs. Mechanistically,...
Abstract The highly glycosylated spike protein of SARS‐CoV‐2 is essential for infection and constitutes a prime target antiviral agents vaccines. pineapple‐derived jacalin‐related lectin AcmJRL present in the medication bromelain significant quantities has previously been described to bind mannosides. Here, we performed large ligand screening by glycan array analysis, quantified interaction with carbohydrates validated high‐mannose glycans as preferred ligands. Because was reported carry...
Abstract The current SARS-CoV-2 pandemic has become one of the most challenging global health threats, with over 530 million reported infections by May 2022. In addition to vaccines, research and development have also been directed towards novel drugs. Since highly glycosylated spike protein is essential for infection, it constitutes a prime target antiviral agents. pineapple-derived jacalin-related lectin (AcmJRL) present in medication bromelain significant quantities previously described...
Abstract Die beiden tetravalenten Lektine LecA und LecB des ESKAPE‐Erregers Pseudomonas aeruginosa sind notwendig für dessen bakterielle Adhäsion, Biofilmbildung Invasion in Wirtszellen. Sie gelten daher als Ansatzpunkte zur medikamentösen antiinfektiösen Therapie. Vor kurzem berichteten wir über hochwirksame divalente Galaktoside spezifische LecA‐Inhibitoren. Diese litten jedoch unter einer sehr geringen Löslichkeit intrinsischen chemischen Instabilität aufgrund zweier Acylhydrazon‐Motive,...