A5075833715- Pineapple and bromelain studies
- Microbial Natural Products and Biosynthesis
- Biochemical and Structural Characterization
- SARS-CoV-2 and COVID-19 Research
- Marine Sponges and Natural Products
- Nasal Surgery and Airway Studies
- Carbohydrate Chemistry and Synthesis
- Phytochemicals and Antioxidant Activities
- Allergic Rhinitis and Sensitization
- Sinusitis and nasal conditions
- Retinal and Optic Conditions
- Genomics, phytochemicals, and oxidative stress
- COVID-19 Clinical Research Studies
- Olfactory and Sensory Function Studies
- Chemical Synthesis and Reactions
- SARS-CoV-2 detection and testing
- Antioxidant Activity and Oxidative Stress
- Inorganic and Organometallic Chemistry
- Plant biochemistry and biosynthesis
- Bioactive Compounds and Antitumor Agents
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Slime Mold and Myxomycetes Research
- Chemical Reactions and Mechanisms
- Protein Tyrosine Phosphatases
URSAPHARM (Germany)
2014-2024
University of Cologne
2021
Max Perutz Labs
2021
University of Vienna
2021
University Hospital Cologne
2021
Saarland University
2006-2013
Helmholtz Institute for Pharmaceutical Research Saarland
2011
University of Stuttgart
1971-1973
Myxococcus xanthus DK1622 is shown to be a producer of myxovirescin (antibiotic TA) antibiotics. The biosynthetic gene cluster spans at least 21 open reading frames (ORFs) and covers chromosomal region approximately 83 kb. In silico analysis ORFs in conjunction with genetic studies suggests the involvement four type I polyketide synthases (PKSs; TaI, TaL, TaO, TaP), one major hybrid PKS/NRPS (Ta-1), number monofunctional enzymes similar ones involved II fatty-acid biosynthesis (FAB). Whereas...
Big effects from small changes: In a comparative analysis of the nonribosomal peptide synthetases (NRPSs) biosynthetic pathways two highly related myxobacterial lipopeptides, module skipping and point mutations are directly correlated to structural variations in these natural products. The process during myxochromide S biosynthesis was characterized biochemically represents first example NRPS systems. Supporting information, including experimental data, for this article is available on WWW...
Under starvation conditions myxobacteria form multicellular fruiting bodies in which vegetative cells differentiate into heat- and desiccation-resistant myxospores. Myxobacteria general are a rich source of secondary metabolites that often exhibit biological activities rarely found nature. Although the involvement yellow compound sporulation body formation Myxococcus xanthus was described almost 30 years ago, chemical principle pigment remained elusive. This work presents isolation structure...
Bromelain has been used for treatment of inflammatory diseases decades. However, the exact mechanism action remains poorly understood. While in vitro investigations have shown conflicting effects on release various cytokines, no vivo data were available. In this study, inflammation‐related cytokines two doses bromelain tested a single dose placebo‐controlled 3 × crossover randomized clinical trial. Cytokine circadian profiles to investigate human immune system by using stimulated whole‐blood...
Proanthocyanidins represent a unique class of oligomeric and polymeric secondary metabolites found ubiquitously in considerable amounts plants some algae. These substances exhibit range rather surprising physical chemical properties which, once applied to living organisms, are translated into multitude biological activities. The latter include antioxidant properties, cancer chemoprevention, anti-inflammatory anti-diabetic effects as well exceptional, yet highly interesting activities, such...
The anti-fungal leupyrrins are secondary metabolites produced by several strains of the myxobacterium Sorangium cellulosum. These intriguing compounds incorporate an atypically substituted γ-butyrolactone ring, as well pyrrole and oxazolinone functionalities, which located within unusual asymmetrical macrodiolide. Previous feeding studies revealed that this novel structure arises from homologation four distinct structural units, nonribosomally-derived peptide, polyketide, isoprenoid a...
Reduction in postoperative edema and inflammatory reactions is the key to posttraumatic regeneration process. Use of bromelain well established this indication, but there some controversy with regard optimal dosing drug. The aim our study was therefore investigate efficacy dosage‐dependent therapy patients after wisdom teeth extraction by comparing registered dosage 1000 FIP (Fédération Internationale Pharmaceutique) against higher dosages 3000 4500 FIP. A total 75 were randomized one three...
Abstract Myxalamids are potent inhibitors of the eukaryotic electron transport chain produced by different myxobacteria. Here, we describe identification myxalamid biosynthesis gene cluster from Myxococcus xanthus . Additionally, new myxalamids ( 5 – 13 ) have been obtained mutasynthesis bkd mutants M. and Stigmatella aurantiaca Moreover, as these still able to produce B 2 ), origin isobutyryl‐CoA (IB‐CoA) starter unit required for its has determined. In a mutant, IB‐CoA originates valine,...
Troxerutin (TRX) is a water-soluble flavonoid which occurs commonly in the edible plants. Recent studies state that TRX improves functionality of nervous system and neutralizes Amyloid-ß induced neuronal toxicity. In this study, an vitro assay based upon Neural stem cell (NSCs) isolated from subventricular zone postnatal balb/c mice was established to explore impact on individual neurogenesis processes general neuroprotective effect against ß-amyloid 1–42 (Aß42) inhibition differentiation...
Abstract With the changing epidemiology of COVID-19 and its impact on our daily lives, there is still an unmet need therapies treating early infections to prevent progression. The current study was a randomized, parallel, double-blind, placebo-controlled trial. Ninety SARS-CoV-2 positive patients were randomized into 3 groups receiving placebo, 0.02% or 0.1% azelastine nasal spray for 11 days, during which viral loads assessed by quantitative PCR. Investigators patients’ status throughout...
Abstract Die Titel‐Verbindungen ( 1,8 ) wurden durch Umsetzung von PCl 5 mit Hexamethylentetramin bzw. 1,3,5‐Trimethylhexahydro‐ s ‐triazin dargestellt. Reaktionen 1 und/oder 8 SbCl . SnCl 4 , Natriummethylat, Natriumacetat, Phenyllithium, Grignard‐Verbindungen, sek. Aminen und Äthanthiol werden beschrieben. Reaktionsprodukte, teilweise bislang nicht beschriebene tertiäre Amine, charakterisiert.
Background. This prospective, controlled, parallel-group observational study investigated the efficacy of a spray containing hyaluronic acid and dexpanthenol to optimise regular treatment after nasal cavity surgery in 49 patients with chronic rhinosinusitis. Methods. The control group received standard therapy. Mucosal regeneration was determined using rhinoscopy sum score (RSS). Pre- postoperative patency tested anterior rhinomanometry. participants were questioned about their symptoms....
Hypertonic saline solutions are in broad medicinal use for treatment of various disorders nose and paranasal sinuses. This trial aimed at the evaluation safety efficacy a hypertonic nasal spray within scope routine care an otorhinolaryngological outpatient setting. One hundred thirty-six patients were included into this uncontrolled clinical received 2.7% solution spray. Symptoms signs documented on ordinal scale 0–4 during initial final examination, both individual symptoms sum symptom...
Abstract The reactivity of 4‐(1‐butoxyvinyl)‐2 H ‐chromen‐2‐one ( 1 ) and E )‐4‐(2‐butoxyvinyl)‐2 2 as diene in thermal Diels–Alder cycloaddition reactions with several electron‐poor dienophiles is reported. Among used this study 1,4‐benzoquinone afforded cycloadducts 11‐butoxy‐1 ‐naphtho[1,2‐ c ]chromene‐1,4,5‐trione 3e 4g that showed Cdc25 phosphatase‐inhibition activity at low micromolar values, both compounds more effective against A C isoforms.
Eine molekulare Ursache der Naturstoffvariation. Ein Vergleich nichtribosomalen Peptidsynthetasen (NRPS) aus den Biosynthesewegen zweier strukturell ähnlicher myxobakterieller Lipopeptide ergab eine Korrelation zwischen dem Überspringen eines Moduls und Punktmutationen mit Strukturvariationen dieser Naturstoffe. Das kompletten während Myxochromid-S-Biosynthese ist das erste Beispiel für Modul-„Skipping“ in einem NRPS-System (siehe Bild). Hintergrundinformationen zu diesem Beitrag sind im WWW...
Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion host proteins. On the other hand, are broadly used industrial applications and resemble promising drug candidates. Bromelain is herbal that medicinally for treatment oedematous swellings inflammatory conditions consists large part proteolytic enzymes. Even though various experiments underline requirement active cysteine biological activity, so far no investigation...
Limited data exist on the clinical benefits of nasal applications for moistening mucosa. We therefore investigated effects hyaluronic acid, acid plus dexpanthenol and isotonic saline sprays in patients suffering from dry nose symptoms an otorhinolaryngological outpatient setting.240 were randomised into this prospective, three-armed trial with two assessment points (baseline 4 weeks later). Patients received either or spray over a period four weeks. Rhinitis Sicca Symptom Score (RSSS) was...
Systemic enzyme therapy with bromelain resembles a sensible alternative to nonsteroidal antiinflammatory drugs for the treatment of sports injuries, particular consideration therapeutic benefits and possible risks. Beyond aftertreatment is used postoperatively as well. Besides desired effects remission oedema pain relief, however, postoperative use raises uncertainty in some patients physicians since an enhanced bleeding tendency case concomitant anticoagulants was described interaction....