A5072818186- Muscle Physiology and Disorders
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Cellular Mechanics and Interactions
- Calpain Protease Function and Regulation
- Congenital heart defects research
- Toxoplasma gondii Research Studies
- Physiological and biochemical adaptations
- Developmental Biology and Gene Regulation
- Protein Tyrosine Phosphatases
- Genetic Neurodegenerative Diseases
- Liver physiology and pathology
- Genetics, Bioinformatics, and Biomedical Research
- Ophthalmology and Eye Disorders
- Autoimmune Neurological Disorders and Treatments
- melanin and skin pigmentation
Umeå University
2016-2024
The pigment pattern of many animal species is a result the arrangement different types pigment-producing chromatophores. zebrafish has three chromatophores: black melanophores, yellow xanthophores, and shimmering iridophores arranged in characteristic golden blue horizontal stripes. In embryo, chromatophores derive from neural crest cells. Using pax7a pax7b mutants, we identified previously unknown requirement for Pax7 xanthophore lineage formation. absence results severe reduction precursor...
Abstract In muscular dystrophies, muscle fibers loose integrity and die, causing significant suffering premature death. Strikingly, the extraocular muscles (EOMs) are spared, functioning well despite disease progression. Although EOMs have been shown to differ from body musculature, mechanisms underlying this inherent resistance dystrophies remain unknown. Here, we demonstrate important differences in gene expression as a response between trunk zebrafish via transcriptomic profiling. We show...
Migrating muscle progenitors delaminate from the somite and subsequently form tissue in distant anatomical regions such as paired appendages, or limbs. In amniotes, this process requires a signaling cascade including transcription factor box 3 (Pax3).In study, we found that, unlike mammals, pax3a/3b double mutant zebrafish develop near to normal appendicular muscle. By analyzing numerous combinations of pax3a, pax3b pax7a, pax7b, determined that there is feedback system compensatory...
Purpose: To study the medial rectus (MR) muscle of zebrafish (Danio rerio) with respect to pattern distribution desmin and its correlation distinct types myofibers motor endplates. Methods: The MRs were examined using confocal microscopy in whole-mount longitudinal specimens cross sections processed for immunohistochemistry antibodies against desmin, myosin heavy chain isoforms, innervation markers. Desmin patterns correlated major myofiber type innervation. A total 1382 nine MR muscles...
The hepatocyte growth factor receptor C-met plays an important role in cellular migration, which is crucial for many developmental processes as well cancer cell metastasis. has been linked to the development of mammalian appendicular muscle, are derived from migrating muscle progenitor cells (MMPs) within somite. Mammalian limbs homologous teleost pectoral and pelvic fins. In this study we used Crispr/Cas9 mutate zebrafish met gene found that MMP musculature paired appendages was severely...
Purpose: The cytoskeleton of the extraocular muscles (EOMs) is significantly different from that other muscles. We aimed to investigate role obscurin, a fundamental cytoskeletal protein, in EOMs. Methods: distribution obscurin human and zebrafish EOMs was compared using immunohistochemistry. two genes zebrafish, obscna obscnb, were knocked out CRISPR/Cas9, investigated immunohistochemistry, qPCR, situ hybridization. optokinetic reflex (OKR) five-day-old larvae adult obscna−/−;obscnb−/−...
We constantly direct our eyes to the object of interest with help extraocular muscles, and thereby use foveal fixation attain best possible visual acuity. The muscles around eye are rather different from other skeletal being, for example, simultaneously fastest in body impossible exhaust. most exciting property is their unique response disease, as they often remain unaffected muscle conditions which lead severe handicap premature death. Understanding coping strategies that allow may provide...
Abstract In muscular dystrophies, muscle fibers loose integrity and die, leading to significant suffering a shorter life. Strikingly, the extraocular muscles (EOMs), controlling eye movements, are spared function well despite disease progression. Although EOMs have been shown important differences compared body musculature mechanisms underlying this inherent resistance dystrophies remain largely unknown. Here, we demonstrate in gene expression as response between trunk zebrafish via...