A5085858278- Surfactants and Colloidal Systems
- Protein Structure and Dynamics
- Receptor Mechanisms and Signaling
- Spectroscopy and Quantum Chemical Studies
- Alzheimer's disease research and treatments
- Mass Spectrometry Techniques and Applications
- Phosphodiesterase function and regulation
- Electrostatics and Colloid Interactions
- Lipid Membrane Structure and Behavior
- Molecular spectroscopy and chirality
- Retinal Development and Disorders
- Supramolecular Self-Assembly in Materials
- Protein Interaction Studies and Fluorescence Analysis
- Proteins in Food Systems
- thermodynamics and calorimetric analyses
- Photochemistry and Electron Transfer Studies
- Trace Elements in Health
- Advanced NMR Techniques and Applications
- Heavy Metal Exposure and Toxicity
- Computational Drug Discovery Methods
- Analytical Chemistry and Chromatography
- Diabetes Treatment and Management
- Hydrogels: synthesis, properties, applications
Stanford University
2019-2024
University of Pennsylvania
2012-2018
Koç University
2013
Family B heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) play important roles in carbohydrate metabolism. Recent structures of family GPCR-Gs complexes reveal a disruption the α-helix transmembrane segment 6 (TM6) not observed A GPCRs. To investigate functional impact this structural difference, we compared structure and function glucagon receptor (GCGR; B) with β2 adrenergic (β2AR; A). We determined GCGR-Gs complex by means cryo-electron microscopy at...
Reverse micelles (RMs) made from water and sodium bis(2-ethylhexyl) sulfosuccinate (AOT) are commonly studied experimentally as models of aqueous microenvironments. They small enough for individual RMs to also be by molecular dynamics (MD) simulation, which yields detailed insight into their structure properties. Although RM size is determined the loading ratio (i.e., molar AOT), experimental measurements imprecise inconsistent, problematic when seeking understand relationship between size,...
Summary G protein-coupled receptors (GPCRs) activate heterotrimeric proteins by stimulating the exchange of guanine nucleotide in Gα subunit. To visualize this mechanism, we developed a time-resolved cryo-EM approach that examines progression ensembles pre-steady-state intermediates GPCR-G protein complex. Using variability analysis to monitor transitions stimulatory Gs complex with β 2 -adrenergic receptor (β AR) at short sequential time points after GTP addition, identified conformational...
Alzheimer's disease is the most common form of dementia. It a neurodegenerative and incurable that associated with tight packing amyloid fibrils. This facilitated by compatibility ridges grooves on surface. The GxMxG motif major factor creating between two surfaces, making it an important target for design aggregation inhibitors. In this study, peptide, experimentally proven to bind Aβ40 fibrils at motif, was mutated novel methodology systematically replaces amino acids residues share...
Previously published experimental studies have suggested that when the 40-residue amyloid beta peptide is encapsulated in a reverse micelle, it folds into structure may nucleate fibril formation (Yeung, P. S.-W.; Axelsen, H. J. Am. Chem. Soc. 2012, 134, 6061 ). The factors induce of this now been identified multi-microsecond simulation same micelle system was studied experimentally. Key features polypeptide-micelle interaction include anchoring hydrophobic residue cluster gaps surface, turn...
Reverse micelles (RMs) made with sodium bis(2-ethylhexyl)sulfosuccinate suspended in isooctane are commonly used experimental models of aqueous microenvironments. However, there important unanswered questions about the very characteristic that makes them interest, namely their size. To explore factors determine size RMs, all-atom molecular dynamics simulations RMs different sizes but same water-loading ratio were performed. An Anton 2 machine was so systems necessary could be extended into...
Reverse micelles (RMs) composed of water and sodium bis(2-ethylhexyl)sulfosuccinate (AOT) in isooctane have a remarkably narrow size distribution around mean value determined by the loading ratio system. It has been proposed that RMs establish this equilibrium either diffusion individual components through phase or cycles fusion fission. To examine these mechanisms, 24 μs all-atom molecular dynamics simulation system containing one small RM large was performed. Results show net movement from...
SUMMARY Heterotrimeric G proteins communicate signals from activated protein-coupled receptors to downstream effector proteins. In the phototransduction pathway responsible for vertebrate vision, protein-effector complex is comprised of GTP-bound transducin α subunit (Gα T ·GTP) and cyclic GMP (cGMP) phosphodiesterase 6 (PDE6), which stimulates cGMP hydrolysis transmit optic nerve. Here we report a cryo-electron microscopy (cryoEM) structure PDE6 complexed Gα . The reveals two ·GTP subunits...