A5052256461- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- DNA and Nucleic Acid Chemistry
- Tissue Engineering and Regenerative Medicine
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Carbon Nanotubes in Composites
- Pickering emulsions and particle stabilization
- Phosphodiesterase function and regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Hemophilia Treatment and Research
- Venous Thromboembolism Diagnosis and Management
- Congenital heart defects research
- Lipid Membrane Structure and Behavior
- Fullerene Chemistry and Applications
- Neuropeptides and Animal Physiology
- Erythrocyte Function and Pathophysiology
Zhejiang University
2022-2024
Peking University
2008-2024
Stanford University
2017-2024
State Key Laboratory of Turbulence and Complex Systems
2022-2024
China Academy of Launch Vehicle Technology
2024
Hebei Academy of Sciences
2024
Southern Medical University
2009-2023
Nanfang Hospital
2022-2023
Huazhong University of Science and Technology
2015-2023
Tongji Hospital
2023
The hybridization kinetics for a series of designed 25mer probe–target pairs having varying degrees secondary structure have been measured by UV absorbance and surface plasmon resonance (SPR) spectroscopy in solution on the surface, respectively. Kinetic rate constants derived from resultant data decrease with increasing probe target similarly both environments. Specifically, addition three intramolecular base slow factor two. For individual strands containing four or more pairs, cannot be...
Abstract Ice-templating technology holds great potential to construct industrial porous materials from nanometers the macroscopic scale for tailoring thermal, electronic, or acoustic transport. Herein, we describe a general ice-templating through freezing material on rotating cryogenic drum surface, crushing it, and then re-casting nanofiber slurry. Through decoupling ice nucleation growth processes, achieved columnar-equiaxed crystal transition in procedure. The highly random stacking...
Abstract Ferroptosis, an iron‐dependent form of regulated cell death driven by excessive accumulation lipid peroxides, has become a promising strategy in cancer treatment. Cancer cells exploit antioxidant proteins, including Ferroptosis Suppressor Protein 1 (FSP1), to prevent ferroptosis. In this study, it is found that the E3 ubiquitin ligase TRIM21 bound FSP1 and mediated its ubiquitination on K322 K366 residues via K63 linkage, which essential for membrane translocation ferroptosis...
Autophagy is an important intracellular catabolic mechanism involved in the removal of misfolded proteins. Atg14L, mammalian ortholog Atg14 yeast and a critical regulator autophagy, mediates production PtdIns3P to initiate formation autophagosomes. However, it not clear how Atg14L regulated. In this study, we demonstrate that ubiquitination degradation controlled by ZBTB16-Cullin3-Roc1 E3 ubiquitin ligase complex. Furthermore, show wide range G-protein-coupled receptor (GPCR) ligands...
Retinoic acid (RA) is a vitamin A metabolite that essential for early embryonic development and promotes stem cell neural lineage specification; however, little known regarding the impact of RA on mRNA transcription microRNA levels differentiation. Here, we present microarray high-output sequencing to clarify how regulates gene expression. Using analysis, showed repressed pluripotency-associated genes while activating ectoderm markers in mouse cells (mESCs). Moreover, modulated DNA...
We present results of the first systematic study on in situ sequence-dependent kinetics for short single-strand oligonucleotide surface immobilization. By measuring film coverage both thiolated and nonthiolated homo-oligomers as a function adsorption time, we determine relative contribution specific thiol-surface nonspecific DNA-surface interactions to overall mechanism DNA-thiol attachment gold. find that play significant role immobilization, influencing not only but also extent oligomer...
Abstract The tumor‐inhibitory effect of C 60 (OH) x was tested on the murine H22 hepatocarcinoma model. Doses 0.2 and 1.0 mg kg −1 body weight both showed significant antitumor activity with tumor inhibition rates 31.9 38.4%, respectively, when mice were treated for 17 consecutive days. damnification liver prominently reduced. Furthermore, histological examination indicated that an envelope fibroblasts lymphocytes formed surrounding tissues in ‐treated group, which inhibited infiltration to...
Summary G protein-coupled receptors (GPCRs) activate heterotrimeric proteins by stimulating the exchange of guanine nucleotide in Gα subunit. To visualize this mechanism, we developed a time-resolved cryo-EM approach that examines progression ensembles pre-steady-state intermediates GPCR-G protein complex. Using variability analysis to monitor transitions stimulatory Gs complex with β 2 -adrenergic receptor (β AR) at short sequential time points after GTP addition, identified conformational...
The potential biomedical applications of fullerenol C(60)(OH)(x) (x≈24) have been extensively studied. However, the structural information interaction with bio-system at molecular level, which is essential for understanding its bioactivity and toxicity, still missing. In this study, lysozyme was selected as a model protein to investigate between biomolecules. A strong induced circular dichroism (CD) signal achiral observed after binding lysozyme. Activity assay shows that activity inhibited...
Human 5-lipoxygenase (5-LOX) is one of the key anti-inflammatory drug targets due to its role in leukotrienes biosynthesis. We have built a model for active conformation human 5-LOX using comparative modeling, docking known inhibitors, and molecular dynamics simulation. Using this model, novel inhibitors were identified by virtual screen. Of 105 compounds tested cell-free assay, 30 IC50 values less than 100 μM 11 10 with strongest inhibition 620 nM. Compounds 4, 7, showed strong activity...
Ischemic stroke is one of the most serious global public health problems. However, performance current therapeutic regimens limited due to their poor target specificity, narrow time window, and compromised effect. To overcome these barriers, we designed an ischemia-homing bioengineered nano-scavenger by camouflaging a catalase (CAT)-loaded self-assembled tannic acid (TA) nanoparticle with M2-type microglia membrane (TPC@M2 NPs) for ischemic treatment.