- Enzyme Catalysis and Immobilization
- Agricultural and Food Sciences
- Catalysis for Biomass Conversion
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Phytase and its Applications
Universidade Federal de São Carlos
2015-2022
In this study, soybean oil deodorizer distillate (SODD), a mixture of free fatty acids and acylglycerides, isoamyl alcohol were evaluated as substrates in the synthesis acid monoesters catalyzed by Eversa (a liquid formulation
Barley β-amylase was immobilized using different techniques. The highest global yield obtained the cross-linked enzyme aggregates (CLEA) technique, employing bovine serum albumin (BSA) or soy protein isolate (SPI) as feeder proteins to reduce diffusion problems. CLEAs produced BSA SPI showed 82.7 ± 5.8 and 53.3 2.4% yield, respectively, a stabilization effect observed upon immobilization at neutral pH value, e.g., after 12 h 55 °C, free is fully inactivated, while retained 25 15% of activity...