A5009627310- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Agricultural and Food Sciences
- Electrochemical sensors and biosensors
- Phytochemistry Medicinal Plant Applications
- Pancreatic function and diabetes
- Carbohydrate Chemistry and Synthesis
- Protein purification and stability
- Lipid metabolism and biosynthesis
- Plant tissue culture and regeneration
- Edible Oils Quality and Analysis
- Microbial Metabolites in Food Biotechnology
- Protein Interaction Studies and Fluorescence Analysis
- Healthcare Regulation
- Calcium Carbonate Crystallization and Inhibition
- Bee Products Chemical Analysis
- Analytical Chemistry and Chromatography
- Magnetic and Electromagnetic Effects
- Minerals Flotation and Separation Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Polymer Surface Interaction Studies
- Transgenic Plants and Applications
Universidade Estadual Paulista (Unesp)
2023-2024
Universidade Federal de São Carlos
2012-2021
Universidade Federal de Alfenas
2015
This work evaluated the immobilization of Candida antarctica lipase B (CALB) on a new heterofunctional support, prepared by silanization silica particles with triethoxy(octyl)silane and trietoxy(3-aminopropyl)silane, followed activation glutaraldehyde. The support (octyl-silica-amino-glutaraldehyde, OSGlu) exhibits features an anionic exchanger hydrophobic adsorbent capable adsorbing covalently linking under mild conditions in single step. As comparison, CALB was also immobilized coated...
Due to the heterogeneity of oils, use mixtures lipases with different activity for a large number glycerol-linked carboxylic acids that compose substrate has been proposed as better alternative than one specific lipase preparation in enzymatic synthesis biodiesel. In this work, from sources were evaluated their soluble form ethanolysis soybean oil. A mixture (50% each lipase, basis) porcine pancreas (PPL) and Thermomyces lanuginosus (TLL) gave highest fatty acid ethyl ester (FAEE) yield...
The preparation of Cross-Linked Enzyme Aggregates (CLEAs) is a simple and cost-effective technique capable generating insoluble biocatalysts with high volumetric activity improved stability. standard CLEA consists the aggregation enzyme its further crosslinking, usually glutaraldehyde. However, some enzymes have too low content surface lysine groups to permit effective crosslinking glutaraldehyde, requiring co-aggregation feeders rich in amino aid formation CLEAs. magnetic particles makes...
The preparation of crosslinked aggregates pancreatic porcine lipase (PPL‐CLEA) was systematically studied, evaluating the influence three precipitants and two crosslinking agents, as well use soy protein an alternative feeder on catalytic properties stability immobilized PPL. Standard CLEAs showed a global yield (CLEA’ observed activity/offered total activity) less than 4%, whereas with addition (PPL:soy mass ratio 1:3) approximately fivefold higher. CLEA PPL prepared glutaraldehyde reagent...
Barley β-amylase was immobilized using different techniques. The highest global yield obtained the cross-linked enzyme aggregates (CLEA) technique, employing bovine serum albumin (BSA) or soy protein isolate (SPI) as feeder proteins to reduce diffusion problems. CLEAs produced BSA SPI showed 82.7 ± 5.8 and 53.3 2.4% yield, respectively, a stabilization effect observed upon immobilization at neutral pH value, e.g., after 12 h 55 °C, free is fully inactivated, while retained 25 15% of activity...
Many approaches have been considered aimed at ethanol production from the hemicellulosic fraction of biomass. However, industrial implementation this process has hindered by some bottlenecks, one most important being ease contamination bioreactor bacteria that metabolize xylose. This work focuses on overcoming problem through fermentation xylulose (the xylose isomer) native Saccharomyces cerevisiae using xylo-oligomers as substrate. A new concept biocatalyst is proposed, containing xylanases...
The synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides applications. This study aimed to evaluate efficiency two different Brazilian green propolis (BGP-AF BGP-AG) as PS aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts their fractions controls....
Stabilization of multimeric enzymes is one the major challenges in biocatalysis, because dissociation subunits can inactivate enzyme. Catalase (CAT; EC 1.11.16) a homotetramer containing Fe-protoporphyrin IX its active site. CAT breaks down hydrogen peroxide into water and molecular oxygen. In this study, cross-linked enzyme aggregates bovine liver (CAT-CLEAs) were prepared. The effects precipitation cross-linking on activity studied. Thermal stability free immobilized also evaluated at 40...
A biocatalyst has been developed for application in the simultaneous isomerization and fermentation (SIF) of xylose, which could enable operation repeated batches use xylose from biomass hemicellulose production second-generation (2G) ethanol. To this end, enzyme isomerase (XI) was immobilized on eleven different supports (based chitosan, modified silica, agarose magnetic supports) to obtain a derivative that is stable under process conditions easy recover fermented medium future industrial...
Hydrophobic immobilization of Burkholderia cepacia lipase onto octyl
Endospermas de mamona (Ricinus communis L.) processados contendo atividade lipolítica têm sido utilizados com sucesso em alguns bioprocessos. Porém, a toxina ricina presente na limita aplicação deste biocatalisador, sendo necessário separá-la da lipase. A primeira etapa processo é solubilização do biocatalisador fase aquosa. Neste contexto, foi avaliada extração lipase diferentes tampões e pHs. O extrato bruto incubado diversos por 12h 25°C; seguido centrifugação para separação endosperma....