A5050873393- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- RNA and protein synthesis mechanisms
- Polysaccharides and Plant Cell Walls
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Carbohydrate Chemistry and Synthesis
- Gut microbiota and health
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Polyamine Metabolism and Applications
- Antimicrobial Peptides and Activities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Plant Pathogens and Fungal Diseases
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Studies on Chitinases and Chitosanases
- Signaling Pathways in Disease
Universidad Complutense de Madrid
2016-2025
Instituto Ramón y Cajal de Investigación Sanitaria
2016-2024
Universitat Pompeu Fabra
2020
Maresme Health Consortium
2020
GTx (United States)
2018
Instituto Cajal
2016
Janssen (Belgium)
2000
University of Minnesota
2000
Consejo Superior de Investigaciones Científicas
1990-1994
University of Otago
1994
Intestinal fungi are an important component of the microbiota, and recent studies have unveiled their potential in modulating host immune homeostasis inflammatory disease. Nonetheless, mechanisms governing immunity to gut fungal communities (mycobiota) remain unknown. We identified CX3CR1+ mononuclear phagocytes (MNPs) as being essential for initiation innate adaptive responses intestinal fungi. MNPs express antifungal receptors activate a Syk-dependent manner. Genetic ablation mice led...
ABSTRACT Amphotericin B (AMB) is an antifungal drug that binds to ergosterol and forms pores at the cell membrane, causing loss of ions. In addition, AMB induces accumulation reactive oxygen species (ROS), although these molecules have multiple deleterious effects on fungal cells, their specific role in action mechanism remains unknown. this work, we studied ROS AMB. We determined intracellular induction 44 isolates different pathogenic yeast ( Candida albicans , parapsilosis glabrata...
ABSTRACT The relevance of the mitogen-activated protein (MAP) kinase Hog1p in Candida albicans was addressed through characterization C. strains without a functional HOG1 gene. Analysis phenotype hog1 mutants under osmostressing conditions revealed that this mutant displays set morphological alterations as result failure to complete final stages cytokinesis, with parallel defects budding pattern. Even permissive conditions, displayed different susceptibility some compounds such nikkomycin Z...
ABSTRACT Candida albicans mutants with mutations in mitogen-activated protein (MAP) kinase HOG1 displayed an increased sensitivity to agents producing reactive oxygen species, such as oxidants (menadione, hydrogen peroxide, or potassium superoxide), and UV light. Consistent this finding, C . Hog1 was activated not only response increase external osmolarity, happens its Saccharomyces cerevisiae homologue, but also peroxide. The Hog1-mediated oxidative stress different from that of...
Mitogen-activated protein (MAP) kinases represent a group of serine/threonine playing central role in signal transduction processes eukaryotic cells. Using strategy based on the complementation thermosensitive autolytic phenotype slt2 null mutants, we have isolated Candida albicans homolog Saccharomyces cerevisiae MAP kinase gene SLT2 (MPK1), which is involved recently outlined PKC1-controlled signalling pathway. The gene, named MKC1 (MAP from C. albicans), coded for putative protein, Mkc1p,...
The Sho1 adaptor protein is an important element of one the two upstream branches high-osmolarity glycerol (HOG) mitogen-activated (MAP) kinase pathway in Saccharomyces cerevisiae, a signal transduction cascade involved adaptation to stress. In present work, we describe its role pathogenic yeast Candida albicans by construction mutants altered this gene. We report here that sho1 are sensitive oxidative stress but has minor transmission phosphorylation Hog1 MAP response stress, which mainly...
The Candida albicans HOG1 gene (HOG1CA) was cloned by functional complementation of the osmosensitive phenotype associated with Saccharomyces cerevisiae hog1 delta mutants. HOG1CA codes for a 377-amino-acid protein, 78% identical to S. Hog1p. A C. null mutant found be sensitive osmotic stress and failed accumulate glycerol on high-osmolarity media.
ABSTRACT The Hog1 mitogen-activated protein (MAP) kinase mediates an adaptive response to both osmotic and oxidative stress in the fungal pathogen Candida albicans . This also participates two distinct morphogenetic processes, namely yeast-to-hypha transition (as a repressor) chlamydospore formation inducer). We show here that repression of filamentous growth occurs under serum limitation other partially inducing conditions, such as low temperature, pH, or nitrogen starvation. To understand...
ABSTRACT Ssk1p of Candida albicans is a putative response regulator protein the Hog1 two-component signal transduction system. In Saccharomyces cerevisiae , phosphorylation state determines whether genes that promote adaptation cells to osmotic stress are activated. We have previously shown C. SSK1 does not complement ssk1 mutant S. and sensitive sorbitol. this study, we show several oxidants, including hydrogen peroxide, t -butyl hydroperoxide, menadione, potassium superoxide when each...
The Candida albicans MKC1 gene encodes a mitogen-activated protein (MAP) kinase, which has been cloned by complementation of the lytic phenotype associated with Saccharomyces cerevisiae slt2 ( mpk1 ) mutants. In this work, physiological role MAP kinase in pathogenic fungus C. was characterized and for biogenesis cell wall suggested based on following criteria. First, mkc1 Δ/ Δ strains displayed alterations their surfaces under specific conditions as evidenced scanning electron microscopy....
Candida albicans is an opportunistic pathogen that has adapted to live and grow in the human body as its natural environment. Under these conditions, this fungus faces numerous challenges, including oxidative, osmotic enzymic processes may damage external internal structures. In view of key role MAP kinase signalling pathways physiology C. , effect agents mimicking vivo environmental conditions on activation p42-44 kinases been analysed. It found Mkc1p phosphorylated presence oxidative...
The role of four mitogen-activated protein (MAP) kinase pathways in the survival Candida albicans following infection human phagocytes has been addressed through analysis mutants defective their respective MAP kinase. While contribution cell integrity (Mkc1-mediated) or mating (Cek2-mediated) is relatively minor to survival, clear and opposite effects were observed for cek1 hog1 mutants, despite fact that these two kinases are important virulence determinants mouse model experimental...
We have characterized the role that Msb2 protein plays in fungal pathogen Candida albicans by use of mutants defective putative upstream components HOG pathway. Msb2, cooperation with Sho1, controls activation Cek1 mitogen-activated kinase under conditions damage cell wall, thus defining as a signaling element this pathway fungus. msb2 display altered sensitivity to Congo red, caspofungin, zymolyase, or tunicamycin, indicating is involved wall biogenesis. (as well Sho1 and Hst7) transmission...
Signal transduction pathways mediated by mitogen-activated protein kinases (MAPKs) play crucial roles in eukaryotic cells. In the pathogenic fungus Candida albicans HOG MAPK pathway regulates response to external stresses (osmotic and oxidative among others) is involved morphogenesis virulence. We show here that lack of Hog1 increases sensitivity this inhibitors respiratory chain. hog1 mutants also an enhanced basal rate compared parental strains, higher levels intracellular reactive oxygen...
ABSTRACT Innate immunity to Candida albicans depends upon the recognition of molecular patterns on fungal cell wall. However, masking major components such as β-glucan seems be a mechanism that fungi have evolved avoid immune through dectin-1 receptor. Although role C. mitogen-activated protein kinase (MAPK) pathways virulence determinants has been established previously with animal models, involved in this behavior is largely unknown. In study we demonstrate disruption extracellular...
The opportunistic pathogen Candida albicans is a frequent inhabitant of the human gastrointestinal tract where it usually behaves as harmless commensal. In this particular niche, needs to adapt different micro environments that challenge its survival within host. order determine those factors involved in gut adaptation, we have used model colonization mouse trace behaviour fungal cells. We developed genetic labelling system based on complementary spectral properties fluorescent proteins GFP...
ABSTRACT Mitochondrial dysfunction in Candida albicans is known to be associated with drug susceptibility, cell wall integrity, phospholipid homeostasis, and virulence. In this study, we deleted CaFZO1 , a key component required during biogenesis of functional mitochondria. Cells FZO1 displayed fragmented mitochondria, mitochondrial genome loss, reduced membrane potential were rendered sensitive azoles peroxide. order understand the cellular response dysfunctional genome-wide expression...
Msb2 is a sensor protein in the plasma membrane of fungi. In human fungal pathogen C. albicans signals via Cek1 MAP kinase pathway to maintain cell wall integrity and allow filamentous growth. doubly epitope-tagged its large extracellular small cytoplasmic domain was efficiently cleaved during liquid surface growth almost quantitatively released into medium. cleavage independent proteases Sap9, Sap10 Kex2. Secreted highly O-glycosylated by mannosyltransferases including Pmt1 resulting an...