A5075304957- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Metabolism, Diabetes, and Cancer
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- Melanoma and MAPK Pathways
- Cellular transport and secretion
- Cancer, Lipids, and Metabolism
- Polyamine Metabolism and Applications
- TGF-β signaling in diseases
- Estrogen and related hormone effects
- Lipid metabolism and biosynthesis
- Amino Acid Enzymes and Metabolism
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Virus-based gene therapy research
- Bone health and treatments
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Cancer Treatment and Pharmacology
- Healthcare Policy and Management
- Protease and Inhibitor Mechanisms
Hunter College
2014-2023
City University of New York
2014-2023
Cornell University
2015-2023
Weill Cornell Medicine
2016-2023
The Graduate Center, CUNY
2004-2023
University of Nottingham Ningbo China
2015-2017
New York Proton Center
2011-2015
Thomson Reuters (United States)
2009-2012
Princess Margaret Cancer Centre
2009
University Health Network
2009
mTOR, the mammalian target of rapamycin, is a critical node for control cell growth and survival has widely been implicated in cancer signals. mTOR exists two complexes: mTORC1 mTORC2. Phospholipase D (PLD) its metabolite phosphatidic acid (PA) have regulation mTOR; however, their role controversial. We report here that suppression PLD prevents phosphorylation substrate S6 kinase (S6K) at Thr389 mTORC2 Akt Ser473. Suppression also blocked insulin-stimulated Ser473 mTORC2-dependent PRAS40....
Constitutive expression of hypoxia-inducible factor (HIF) has been implicated in several proliferative disorders. HIF1 alpha and HIF2 linked to a number human cancers, especially renal cell carcinoma (RCC), which is the more important contributor. Expression dependent on mammalian target rapamycin (mTOR) sensitive rapamycin. In contrast, there have no reports linking with mTOR. mTOR exists two complexes, mTORC1 mTORC2, are differentially We report here that although clear differences...
Treatment of cells with tumor-promoting phorbol esters results in the activation but then depletion ester-responsive protein kinase C (PKC) isoforms. The ubiquitin-proteasome pathway has been implicated regulating levels many cellular proteins, including those involved cell cycle control. We report here that 3Y1 rat fibroblasts, proteasome inhibitors prevent PKC isoforms α, δ, and ɛ response to ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Proteasome also blocked effects TPA on...
Most genetic changes that promote tumorigenesis involve dysregulation of G1 cell cycle progression. A key regulatory site in is a growth factor-dependent restriction point (R) where cells commit to mitosis. In addition the "R," which maps about 3.5 hours after mitosis, there another checkpoint later dependent on nutritional sufficiency has also been referred as R. However, this second late can be distinguished both temporally and genetically from We are proposing more appropriately "cell...
During the winter of 1989–1990, influenza type A(H3N2) circulated widely, causing excess morbidlty and mortality nationwide. From November through April, hospitalized cases pneumonia occurring among noninstitutionalized individuals 65 or more years age were identified by 20 acute care hospitals in southern lower Michigan. These group matched on age, sex, race, zip code to randomly sampled, community-based controls from a comprehensive listing Medicare beneficiaries residing study area....
In response to epidermal growth factor (EGF), the EGF receptor is endocytosed and degraded. A substantial lag period exists between endocytosis degradation, suggesting that more than a simple negative feedback. Phospholipase D (PLD), which has been implicated in vesicle formation Golgi, activated other factors. We report here dependent upon PLD PLD1 regulators, protein kinase C alpha RalA. EGF-induced degradation accelerated by overexpression of either wild-type or PLD2 retarded...
Tumor-promoting phorbol esters activate, but then deplete cells of, protein kinase C (PKC) with prolonged treatment. It is not known whether ester-induced tumor promotion due to activation or depletion of PKC. In rat fibroblasts overexpressing the c-Src proto-oncogene, ester 12-O-tetradecanoylphorbol-13-acetate (TPA) induced anchorage-independent growth and other transformation-related phenotypes. The appearance transformed phenotypes by TPA in these correlated rather expressed PKC isoforms....
Since the role of saccharides in cell recognition, metabolism, and labeling is well-established, conjugation to drugs an active area research. Thus, one goal use saccharide-drug conjugates impart a greater specificity toward given type or other targets. Although widely used treat some cancers age related macular degeneration, photodynamic therapy (PDT) display poor chemical selectivity intended targets, uptake by cells most likely arises from passive, diffusional processes. Instead, specific...
In several neuronal cell systems, fibroblast-derived growth factor (FGF) and nerve (NGF) act as neurogenic agents, whereas epidermal (EGF) acts a mitogen. The mechanisms responsible for these different cellular fates are unclear. We report here that although FGF, NGF, EGF all activate mitogen-activated protein (MAP) kinase (extracellular signal-related [ERK]) in rat hippocampal (H19-7) pheochromocytoma (PC12) cells, the activation of ERK by agents FGF NGF is dependent upon Cdelta (PKCdelta),...
MDA-MB-231 human breast cancer cells belong to a highly invasive metastatic cell line that depends on phospholipase D (PLD) activity for survival when deprived of serum growth factors. In response the stress withdrawal, there is rapid and dramatic increase in PLD activity. Concomitant with increased activity, was an ability both migrate invade Matrigel. The Matrigel dependent mTOR, downstream target signals. Serum withdrawal also led PLD-dependent expression factor, hypoxia-inducible...