A5108008421- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
The University of Texas MD Anderson Cancer Center
2019-2022
The University of Texas Health Science Center at Houston
2019-2020
Thermo Fisher Scientific (Israel)
2014
Abstract Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated AsCpf1 can be used for functional genomics screenings and an AsCpf1-based multiplexed performs similarly as compared to currently available monocistronic CRISPR/Cas9 libraries, with only one vector required each gene. construct smallest whole-genome CRISPR knock-out...
High-throughput RNA sequencing enables quantification of transcripts (both known and novel), exon/exon junctions fusions exons from different genes. Discovery gene fusions–particularly those expressed with low abundance– is a challenge short- medium-length reads. To address this challenge, we implemented an RNA-Seq mapping pipeline within the LifeScope software. We introduced new features including filter junction mapping, annotation-aided pairing rescue accurate quality values. combined...
Myxoid/round-cell liposarcoma (MLS/RCLS) is characterized by either the fusion gene FUS-DDIT3 or less commonly occurring EWSR1-DDIT3 and most cases carry few no additional cytogenetic changes. There are conflicting reports concerning status role of TP53 in MLS/RCLS. Here we analysed four MLS/RCLS derived cell lines for mutations, expression function. Three SV40 transformed expressed normal proteins. Irradiation caused posttranslational modifications induced P21 two these lines. Transfection...
ABSTRACT Cancers are highly complex ecosystems composed of molecularly distinct sub-populations tumor cells, each exhibiting a unique spectrum genetic features and phenotypes, embedded within organ context. To substantially improve clinical outcomes, there is need to comprehensively define inter- intra-tumor phenotypic diversity, as well understand the dependencies that underlie discrete molecular subpopulations. this end, we integrated CRISPR-based co-dependency annotations with...
Abstract Introduction: Pre-existing tumor heterogeneity may allow tumors to escape treatment via selection and expansion of drug-resistant clones. Recent discovery KRAS targeted therapies (e.g. SOS1i, KRASG12Ci, etc.) has dramatically changed the clinical outlook for patients with mutant tumors. Here, we generated utilized pancreatic clonal replica (CRTs) identify clones inhibitors SOS1i MEKi, KRASG12Di). Deep characterization inhibition revealed a novel epigenetic vulnerability which inform...