A5031023052- Hepatitis B Virus Studies
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- interferon and immune responses
- Antimicrobial Resistance in Staphylococcus
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis Viruses Studies and Epidemiology
- Cytokine Signaling Pathways and Interactions
- Natural product bioactivities and synthesis
- Liver Disease Diagnosis and Treatment
- Pharmacological Effects of Natural Compounds
- Malaria Research and Control
- HIV Research and Treatment
- Berberine and alkaloids research
- Insect symbiosis and bacterial influences
- Cancer Research and Treatments
- Galectins and Cancer Biology
- HIV/AIDS Research and Interventions
- Carbohydrate Chemistry and Synthesis
- Essential Oils and Antimicrobial Activity
- HIV/AIDS drug development and treatment
- Antibiotic Resistance in Bacteria
- Cancer therapeutics and mechanisms
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
Kunming Third People's Hospital
2012-2016
Army Medical University
2015
Kunming General Hospital of Chengdu Military Command
2012
Antibacterial activity of berberine (Ber) and 8-acetonyl-dihydroberberine (A-Ber) alone combined uses with antibacterial agents ampicillin (AMP), azithromycin (AZM), cefazolin (CFZ) levofloxacin (LEV) was studied on 10 clinical isolates SCCmec III type methicillin-resistant Staphylococcus aureus (MRSA). Susceptibility to each agent tested using a broth microdilution method the chequerboard time-kill tests for evaluations, respectively. The MICs/MBCs (mg/mL) ranges were 32–128/64–256...
The in vitro antimicrobial activities of 30 Chinese medicinal plants were evaluated with reference to the treatment record infectious diseases Traditional Medicine (TCM) literature. plant materials extracted 80% ethanol and extracts primarily screened against conventional clinical pathogens like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa Candida albicans by agar diffusion method. Their inhibition zone diameters (IZDs, mm, 50 mg/mL) ranged from 2,048 standard broth...
Through bioassay-guided fractionation of the extracts from aerial parts Chinese herb Hypericum japonicum Thunb. Murray, Isojacareubin (ISJ) was characterized as a potent antibacterial compound against clinical methicillin-resistant Staphylococcus aureus (MRSA). The broth microdilution assay used to determine minimal inhibitory concentrations (MICs) and bactericidal (MBCs) ISJ alone. results showed that its MICs/MBCs ranged 4/16 16/64 μg/mL, with required inhibit or kill 50% strains...
Background/Aims: Signal transducer and activator of transcription (STAT) pathway plays an important role in antiviral efficacy interferon alpha (IFN-α). IFN-α is the main therapeutic against hepatitis C virus (HCV) infection. We explored effects on HCV replication gene expression by targeting STAT. Methods: In response to IFN-α, STAT status, replication, were analyzed human hepatoma Huh7.5.1 cells before after cell culture-derived Results: treatment induced phosphorylation STAT1 STAT2 cells....
Hepatitis B virus (HBV) infection is a major health concern with more than two billion individuals currently infected worldwide. Because of the limited effectiveness existing vaccines and drugs, development novel antiviral strategies urgently needed. Heat stress cognate 70 (Hsc70) an ATP-binding protein heat family. Hsc70 has been found to be required for HBV DNA replication. Here we report, first time, that combined siRNAs targeting viral gene siHsc70 are highly effective in suppressing...
Aim: To observe the influence of HCV F protein on apoptosis HepG2 cells, and explore association between NF‐κB signal pathway. Methods: 1b gene containing HepG2‐F cells C HepG2‐C were treated with 100 IU/mL TNF‐α, analyzed by flow cytometry, Western blotting, dual luciferase reporter assay. Empty plasmid pcDNA3.1 + HepG2‐3.1 used as control. Results: (i) With treatment TNF‐α for 18 h, rates (AR) 0.41% (± 0.11%) 37.43% 2.03%) respectively, while that was 4.07% 0.18%). At 36 h after treatment,...
The most common antiretroviral treatment (ART) received by individuals infected with HIV-1 in China is the combination therapy, comprised of nucleoside reverse transcriptase inhibitors (NRTIs) and nonnucleoside (NNRTIs). To assess prevalence drug resistance subtypes Honghe Yunnan, China, patient plasmas from ART-failed were collected January 2010 to December 2012. Genotyping was conducted using an in-house assay on plasmas. A total 254 pol sequences obtained. 47.2% individuals. Of these...
Objective The vaccines currently developed against infectious diseases fail to induce effective antiviral immune responses control an abrupt outbreak of viral epidemic in a short space time.Hence there is urgent need develop emergency capable producing prophylactic effects diseases.RNA interference(RNAi)is mechanism that inhibits gene expression by causing the degradation specific RNA molecules or hindering transcription genes.The rapidity and uniqueness RNAi can make up for current...