A5036634694- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Insect Utilization and Effects
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Insect and Arachnid Ecology and Behavior
- Identification and Quantification in Food
- Insect and Pesticide Research
- Protein Hydrolysis and Bioactive Peptides
- Bacteriophages and microbial interactions
- Meat and Animal Product Quality
- Brucella: diagnosis, epidemiology, treatment
- Herbal Medicine Research Studies
- Animal and Plant Science Education
- Immunotherapy and Immune Responses
- Probiotics and Fermented Foods
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Antimicrobial Resistance in Staphylococcus
James Cook University
2014-2024
Australian Institute of Tropical Health and Medicine
2016-2024
Murdoch Children's Research Institute
2020-2024
ENT and Allergy
2023
Royal Children's Hospital
2020
University of Newcastle Australia
2009-2013
Understanding and predicting an individual's clinical cross-reactivity to related allergens is a key better management, treatment progression of novel therapeutics for food allergy. In allergy, observed in patients reacting unexpected allergen sources containing the same allergenic protein or antibody binding patches (epitopes), often resulting severe allergic reactions. Shellfish allergy affects up 2% world population persists life most patients. The diagnosis shellfish however challenging...
Insects have been consumed by people for millennia and recently proposed as a complementary, sustainable source of protein to feed the world's growing population. crustaceans both belong arthropod family. Crustacean (shellfish) allergies are common potentially severe; hence, cross-reactivity immune system with insect proteins is potential health concern. Herein, LC-MS/MS was used explore proteome whole, roasted whole powdered cricket products. Eight extraction protocols were compared using...
Antibiotic resistance is a growing global problem, with very few new compounds in development. Bacterial transcription an underutilized target for antibiotics, which has been attributed to the similarity of active site RNA polymerases (RNAPs) across all domains life and ease can arise through point mutation at multiple sites within this conserved region. In study we have taken rational approach design novel set that specifically formation initiation complexes by preventing unique bacterial σ...
Despite recent technological advances, novel allergenic protein discovery is limited by their low abundance, often due to specific physical characteristics restricting recovery during the extraction process from various allergen sources. In this study, eight different buffers were compared for ability recover proteins Pacific oyster (Crassostrea gigas). The composition was investigated using high resolution mass spectrometry. antibody IgE-reactivity of each extract determined a pool serum...
Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need feed growing population. Tropomyosin is main allergen; however, additional potential allergens not well characterized and impact of extraction procedures on immunological reactivity unknown.
Shellfish allergy affects 2% of the world's population and persists for life in most patients. The diagnosis shellfish allergy, particular shrimp, is challenging due to similarity allergenic proteins from other invertebrates. Despite clinical importance immunological cross-reactivity among species between invertebrates such as dust mites, underlying molecular basis not well understood. Here we mine complete transcriptome five frequently consumed shrimp identify compare allergens with all...
RNA polymerase (RNAP) is an essential and highly conserved enzyme in all organisms. The process of transcription initiation fundamentally different between prokaryotes eukaryotes. In prokaryotes, regulated by sigma factors, making the interaction factors RNAP attractive target for antimicrobial agents. Our objective was to achieve first step developing novel agents, namely prove experimentally that a bacterial factor can be disrupted introducing carefully designed mutations into sigma(A)...
The Pacific oyster is a commercially important mollusc and, in contrast to most other shellfish species, frequently consumed without prior heat treatment. Oysters are rich many nutrients but can also cause food allergy. Knowledge of their allergens and cross-reactivity remains very limited. These limitations make an optimal diagnosis allergy difficult, particular the (Crassostrea gigas), cultivated species worldwide. This study aimed characterise IgE sensitisation profiles 21...
Background: The increasing prevalence of food allergies requires development specific and sensitive tests capable identifying the allergen responsible for disease. serologic that can detect IgE antibodies to allergenic proteins would, therefore, be highly received. Results: Here we present two new quantitative immuno-PCR assays detection shrimp tropomyosin. Both are based on self-assembling Tus-Ter-lock protein–DNA conjugation system. Significantly elevated levels tropomyosin-specific were...
Exploration of important insect proteins — including allergens and proteomes can be limited by protein extraction buffer selection the complexity proteome. Herein, LC-MS/MS-based proteomics experiments were used to assess efficiencies for a suite buffers effect ingredient processing on proteome allergen detection. Discovery revealed that SDS-based yields maximum number groups from three types BSF samples. Bioinformatic analysis composition could influence Upon applying multi-level filtering...
Due to the widespread use of shellfish ingredients in food products, accurate labelling is urgently needed for consumers with allergies. Most crustacean allergen detection systems target immunorecognition allergenic protein tropomyosin. However, this mode may be affected by an origin-dependent composition. This study determined if geographic location capture, or aquaculture, influenced profiles Black Tiger Shrimp (Penaeus monodon), one most farmed and consumed shrimp species worldwide....
Food allergy is becoming a major and increasing health burden worldwide. Seafood one of the most common causes anaphylaxis, but currently there are limited diagnostic methods, which do not reflect diversity fish shellfish species consumed globally. In Australasia, skin prick testing (SPT) commonly used method for in vivo diagnosis. There many factors limiting utility SPT. Appropriate species-specific preparations often available, those that may be well standardised allergen content. We aimed...
Increasing production and consumption of mollusc is associated with the rise in prevalence allergy worldwide, currently ranging from 0.15% to 1.3% general population. However, elucidation allergens for better diagnostics still lags behind other seafood groups such as fish crustacean. Genomic data have been utilized previously improved identification non-food by performing similarity searching using BLAST program. Based on published genome Pacific oyster (Crassostrea gigas) we aimed identify...
There is an increasing prevalence of food allergies world-wide. The correct diagnosis can be difficult so the development specific and sensitive serologic tests are in high demand. We have developed a diagnostic platform that utilises Tus-Ter Immuno-PCR system, based on self-assembling Tus-Ter-lock protein-DNA conjugation system. This enables detection IgE antibodies to clinically relevant allergenic proteins. new technique was trialled by testing for reactions against tropomyosin, major...
Abstract Shellfish allergy affects up to 2% of the world’s population and persists for life in most patients. The diagnosis a shellfish allergy, particular shrimp, is however often challenging due similarity allergenic proteins other invertebrates. Despite clinical importance, complete allergen repertoire allergy-causing shrimps remains unclear. Here we mine transcriptome five frequently consumed shrimp species identify compare allergens with all known sources. transcriptomes were assembled...