A5029225979- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
- Immune Cell Function and Interaction
- Dermatology and Skin Diseases
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Indoor Air Quality and Microbial Exposure
- TGF-β signaling in diseases
- Monoclonal and Polyclonal Antibodies Research
- Neonatal Respiratory Health Research
- Occupational exposure and asthma
- Cancer Immunotherapy and Biomarkers
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Lipid Membrane Structure and Behavior
- Drug-Induced Adverse Reactions
- Inhalation and Respiratory Drug Delivery
- Antimicrobial Peptides and Activities
- Kruppel-like factors research
- Bioactive Compounds and Antitumor Agents
Monash University
2015-2024
Alfred Health
2018-2024
The Alfred Hospital
2011-2021
Melbourne Clinic
2020
Center for Visual Communication (United States)
2016
Flinders University
2010
Charles Darwin University
2010
Menzies School of Health Research
2010
The Kids Research Institute Australia
2010
The University of Western Australia
2010
The present study was undertaken to examine the effect of prolonged running on monocyte intracellular cytokine production and plasma concentration. Blood samples were collected 1 h before, immediately after, 2 24 after a competitive marathon run. There no change in number cells spontaneously producing tumor necrosis factor (TNF)-alpha; however, there decrease interleukin (IL)-1alpha IL-6 (P < 0.01) postexercise. In contrast, an increase monocytes that responded lipopolysaccharide stimulation...
Sublingual allergen-specific immunotherapy is gaining popularity for treatment of allergic diseases, but underlying immunological mechanisms are unresolved.To perform detailed investigation sublingual house dust mite (HDM) immunotherapy.A 12-month randomized double-blind placebo-controlled study HDM in 30 HDM-allergic subjects was performed, with 1-year open extension 9 patients on active treatment. HDM-stimulated blood mononuclear cells were analyzed proliferation, cytokines, and regulatory...
Scope Prawn allergy is one of the leading causes I g E ‐mediated hypersensitivity to food. Alterations ‐antibody reactivity prawn allergens due thermal processing are not fully understood. The aim this study was analyze impact heating on using a comprehensive allergenomic approach. Methods and results Proteins from raw heat‐processed black tiger ( P enaeus monodon ) extracts as well recombinant tropomyosin (rPen m1) were analyzed by SDS ‐ PAGE immunoblotting sera 16 shellfish allergic...
Summary Background Peanut and tree nut allergy is characterized by a high frequency of life‐threatening anaphylactic reactions typically lifelong persistence. more common than allergy, but many subjects develop hypersensitivity to both peanuts nuts. Whether this due the presence cross‐reactive allergens remains unknown. Objective The aim study was investigate allergenic cross‐reactivity between peanut Methods Western blotting ELISA were performed using sera from with or without assess...
Ten women with infertility, regular menses, and elevated plasma FSH concentrations after a failed in vitro fertilization attempt were studied throughout spontaneous menstrual cycle. Plasma estradiol, progesterone, inhibin, LH, measured by RIA on days 1, 8, 15, 22 compared the ovarian steroid gonadotropin profiles obtained from seven endocrine-normal women. The hypergonadotropic group not associated significant changes E2 P4, but an increase LH was found (medians of 18 4, 17 6, 7 <3 U/L for...
The present study was undertaken to examine the effect of exercise and carbohydrate (CHO) ingestion on intracellular monocyte cytokine production. Subjects performed 2 h cycling at 70 % peak pulmonary O2 uptake (VO2,peak) while ingesting either an 8 CHO beverage or a sweet placebo. Whole blood incubated with (stimulated) without (spontaneous) lipopolysaccharide (LPS) surface stained for antigens. cells were permeabilised, cytokines analysed using flow cytometry. Exercise had no number...
There is increasing interest in the use of engineered particles for biomedical applications, although questions exist about their proinflammatory properties and potential adverse health effects. Lung macrophages dendritic cells (DC) are key regulators pulmonary immunity, but little known uptake different sized or nature induced immunological imprint. We investigated comparatively imprints inert nontoxic polystyrene nanoparticles 50 nm diameter (PS50G) 500 (PS500G). Following intratracheal...
ABSTRACT Scabies, a parasitic skin infestation by the burrowing “itch” mite Sarcoptes scabiei , causes significant health problems for children and adults worldwide. Crusted scabies is particularly severe form of in which mites multiply into millions, causing extensive crusting. The symptoms signs suggest host immunity to mite, but specific resistant response humans remains largely uncharacterized. We used 4 recombinant proteins with sequence homology extensively studied house dust allergens...
Shellfish allergy is a major cause of food-induced anaphylaxis, but the allergens are not well characterized. This study examined effects heating on blue swimmer crab (Portunus pelagicus) in comparison with those black tiger prawn (Penaeus monodon) by testing reactivity shellfish-allergic subjects' serum IgE. Cooked extracts both species showed markedly increased IgE ELISA and immunoblotting, clinical relevance was confirmed basophil activation tests. Inhibition immunoblotting demonstrated...
While treatment for atopic rhinitis is aimed mostly to relieve symptoms, only allergen-specific immunotherapy (AIT) targeted modify the natural history of allergic diseases. This results in sustained clinical tolerance, even when has stopped. The immunomodulatory effects AIT are attributed mainly increased regulatory T-cell function and IgG4 , yet little known about effect on memory B-cell compartment.We examine IgE- IgG subclass-expressing B cells.We recruited 29 patients with seasonal...
Abstract Background Sublingual immunotherapy (SLIT) for grass pollen allergy can modify the natural history of allergic rhinitis and is associated with increased allergen‐specific IgG 4 . competitively inhibits functional IgE on surface effector cells, such as mast cells basophils, from binding to allergens. To further understand important role memory B‐cell (Bmem) responses play in mediating beneficial effects SLIT, we assessed changes Bmem subsets induced by SLIT allergy. Methods Blood...
Sixty cases of colonic carcinoma have been investigated for antitumour immunoreactivity. The tests employed were blood lymphocyte reactivity and complement-dependent serum cytotoxicity against cultured tumour cells, immunofluorescence membrane staining viable cells cytoplasmic dried in films. Nineteen positive by one or more the most frequent response, lymphocytotoxicity, was detected 8 24 tested this way. lymphocytotoxicity persisted a case three times over year. Immunoreactivity cell...
Summary Background Peanut allergy is a life‐threatening condition; there currently no cure. While whole allergen extracts are used for specific immunotherapy many allergies, they can cause severe reactions and even fatalities in peanut allergy. Objective To identify short, HLA‐degenerate CD4 + T cell epitope‐based peptides of the major Ara h 1 that target allergen‐specific cells without causing IgE‐mediated inflammatory activation, as candidates safe peanut‐specific immunotherapy. Methods...
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen not fully appreciated. This likely due to insolubility neutral aqueous solutions, leading low abundance commercially available vitro skin prick tests for fish allergy.To investigate of a large patient population (n = 101).Acid-soluble type I was extracted from muscle Atlantic salmon, barramundi, yellowfin tuna. IgE binding analyzed by ELISA 101 fish-allergic patients....
Despite recent technological advances, novel allergenic protein discovery is limited by their low abundance, often due to specific physical characteristics restricting recovery during the extraction process from various allergen sources. In this study, eight different buffers were compared for ability recover proteins Pacific oyster (Crassostrea gigas). The composition was investigated using high resolution mass spectrometry. antibody IgE-reactivity of each extract determined a pool serum...