A5052888347- Cancer Immunotherapy and Biomarkers
- Cutaneous Melanoma Detection and Management
- Immunotherapy and Immune Responses
- Food Quality and Safety Studies
- Meat and Animal Product Quality
- Analytical Chemistry and Chromatography
- Epigenetics and DNA Methylation
- Microbial Metabolism and Applications
- Mycotoxins in Agriculture and Food
- Advanced Glycation End Products research
- Signaling Pathways in Disease
- Plant Growth Enhancement Techniques
- Bee Products Chemical Analysis
- Lung Cancer Research Studies
- Geomechanics and Mining Engineering
- Tea Polyphenols and Effects
- Toxin Mechanisms and Immunotoxins
- Botanical Research and Applications
- Fungal Biology and Applications
- Nanocomposite Films for Food Packaging
- Chromatography in Natural Products
- Lung Cancer Treatments and Mutations
- Identification and Quantification in Food
- Heat shock proteins research
- Healthcare and Venom Research
Shanghai Ocean University
2024
Third Affiliated Hospital of Sun Yat-sen University
2019
Sun Yat-sen University
2019
Xinyang College of Agriculture and Forestry
2006-2012
Nanchang University
2007
Huazhong Agricultural University
2006
IntroductionVariable genomic breakpoints have been identified through the application of target-capture DNA next-generation sequencing (NGS) for ALK, ROS1, and RET fusion detection in NSCLC. We investigated whether breakpoint location can predict matched targeted therapy efficacy.MethodsNSCLCs were analyzed by NGS, target-specific RNA whole-transcriptome sequencing, immunohistochemistry.ResultsIn total, 3787 NSCLC samples analyzed. NGS detected fusions 241, 59, 76 cases, respectively. These...
Joint contracture is a fibrous disease characterized as joint capsule fibrosis that results in dysfunction and disability. The purpose of this study was to analyze the biological activities chaperonin containing T-complex polypeptide (CCT) subunits determine role CCT chaperone rat model.In study, model established by immobilizing knee for 8 weeks. Then, fibroblasts were isolated from posterior cultured functional analysis such qRT-PCR, Western blot, transwell assay, collagen assay. effect...
Abstract Mucosal melanoma exhibits limited responsiveness to anti-PD-1 therapy. However, a subgroup of mucosal melanomas, particularly those situated at specific anatomic sites like primary malignant the esophagus (PMME), display remarkable sensitivity treatment. The underlying mechanisms driving this superior response and DNA methylation patterns in have not been thoroughly investigated. We collected tumor samples from 50 patients with melanoma, including 31 PMME 19 non-esophageal (NEMM)....
Abstract BACKGROUND N ε‐carboxymethyllysine (CML), ε‐carboxyethyllysine (CEL) and α ‐aminoadipic acid (AAA) are important foodborne hazards their intake can cause a variety of diseases in humans. It is extremely to investigate the formation mechanism CML, CEL AAA, as well association with each other when aiming control production. RESULTS A multi‐response kinetic model was developed within glucose‐lysine Maillard reaction system. The concentrations glucose, lysine, glyoxal (GO),...
<p>Association of epigenetic features with prognosis in patients PMME. <b>A,</b> Comparison methylation levels selected DMRs (statistically significant multivariate analysis) among PMME, NEMM, and normal controls. <b>B,</b> Kaplan–Meier curve OS PMME stratified on the basis risk score derived from 7-DMR panel–based COX model. <b>C,</b> ROC depicting prediction performance model for survival status at 12, 18, 24 months, assessed using LOOCV. entire...
<p>Supplementary Figure S2. Diagram illustrating the genomic loci of 7-DMR panel.</p>
<p>Demographic and clinical characteristics of the patients with PMME NEMM included in study</p>
<p>More M1 and M2 macrophages, CD8<sup>+</sup> T cells, activated CD4<sup>+</sup> memory cells infiltrated in PMME compared with NEMM. <b>A,</b> Representative mIF image of macrophages. <b>B,</b> cells. SOX10, CD68, CD45RO, CD69 as markers for melanoma T, respectively. CD80 was employed to label while CD163 CD206 were used The ratio (<b>C</b>) (<b>D</b>) total macrophages PMME, NEMM, controls. positive rate...
<p>Association between <i>TERT</i> hypermethylation and immunotherapy responses. <b>A,</b> Significant immune-related pathways that were derived from GSEA of methylation-related genes when comparing PMME NEMM. <b>B,</b> normal controls. The infiltration level differences CD8<sup>+</sup> T cells (<b>C</b>) activated CD4<sup>+</sup> memory (<b>D</b>) in patients with...
<p>Increased immune cell infiltration and enriched immune-related pathways in PMME compared with NEMM. <b>A,</b> Comparison of infiltrated cells between <b>B,</b> Significantly identified through GSEA when comparing NEMM.</p>
<p>Association between <i>TERT</i> hypermethylation and immunotherapy responses. <b>A,</b> Significant immune-related pathways that were derived from GSEA of methylation-related genes when comparing PMME NEMM. <b>B,</b> normal controls. The infiltration level differences CD8<sup>+</sup> T cells (<b>C</b>) activated CD4<sup>+</sup> memory (<b>D</b>) in patients with...
<div>Abstract<p>Mucosal melanoma exhibits limited responsiveness to anti-PD-1 therapy. However, a subgroup of mucosal melanomas, particularly those situated at specific anatomic sites like primary malignant the esophagus (PMME), display remarkable sensitivity treatment. The underlying mechanisms driving this superior response and DNA methylation patterns in have not been thoroughly investigated. We collected tumor samples from 50 patients with melanoma, including 31 PMME 19...
<p>Supplementary Figure S2. Diagram illustrating the genomic loci of 7-DMR panel.</p>
<p>Supplementary Table 1. The univariate and multivariate COX analyses for OS associated DMRs in PMME patients.</p>
<p>PMME had a distinct epigenetic profile when compared with NEMM and normal controls. <b>A,</b> Schematic representation of the study design methodology. Methyl-seq, methylation sequencing; mIF, multiplex immunofluorescence; QC, quality control. <b>B,</b> The number hypermethylated hypomethylated DMRs between PMME, NEMM, <b>C,</b> unsupervised clustering in promoter regions PMME <b>D,</b> NEMM.</p>
<p>Role of <i>TERT</i> hypermethylation in PMME and its impact on immunologic signatures. <b>A,</b> Heat map depicting the association between hypermethylated genes immune cell infiltration PMME. <b>B,</b> Linear regression analysis demonstrating correlation methylation <b>C,</b> Comparison levels among PMME, NEMM, controls (*, <i>P</i> < 0.05; **, 0.01; ***, 0.001). <b>D,</b> expression tumors, controls.</p>
<p>Role of <i>TERT</i> hypermethylation in PMME and its impact on immunologic signatures. <b>A,</b> Heat map depicting the association between hypermethylated genes immune cell infiltration PMME. <b>B,</b> Linear regression analysis demonstrating correlation methylation <b>C,</b> Comparison levels among PMME, NEMM, controls (*, <i>P</i> < 0.05; **, 0.01; ***, 0.001). <b>D,</b> expression tumors, controls.</p>
<div>Abstract<p>Mucosal melanoma exhibits limited responsiveness to anti-PD-1 therapy. However, a subgroup of mucosal melanomas, particularly those situated at specific anatomic sites like primary malignant the esophagus (PMME), display remarkable sensitivity treatment. The underlying mechanisms driving this superior response and DNA methylation patterns in have not been thoroughly investigated. We collected tumor samples from 50 patients with melanoma, including 31 PMME 19...
<p>Supplementary Figure S1. PMME were generally associated with relatively lower levels of gene expression. (A) Vocanal plot and (B) unsupervised clustering the top 50 differentially expressed (DE) genes between normal control samples. (C) (D) DE NEMM.</p>
<p>Supplementary Figure S1. PMME were generally associated with relatively lower levels of gene expression. (A) Vocanal plot and (B) unsupervised clustering the top 50 differentially expressed (DE) genes between normal control samples. (C) (D) DE NEMM.</p>
<p>Increased immune cell infiltration and enriched immune-related pathways in PMME compared with NEMM. <b>A,</b> Comparison of infiltrated cells between <b>B,</b> Significantly identified through GSEA when comparing NEMM.</p>
<p>Supplementary Table 1. The univariate and multivariate COX analyses for OS associated DMRs in PMME patients.</p>
<p>Supplementary Table 2. The immune-related pathways that were enriched in PMME when compared with NEMM using GSEA analysis.</p>
<p>Supplementary Table 2. The immune-related pathways that were enriched in PMME when compared with NEMM using GSEA analysis.</p>
<p>Supplementary Table 3. The immune-related pathways that were enriched in TERT methylation high group versus low using GSEA analysis.</p>