A5022094524- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Alzheimer's disease research and treatments
- Pancreatic function and diabetes
- Sleep and Wakefulness Research
- Metabolism, Diabetes, and Cancer
- Phosphodiesterase function and regulation
- Histone Deacetylase Inhibitors Research
- Receptor Mechanisms and Signaling
- Motor Control and Adaptation
- Healthcare and Venom Research
- Engineering Education and Technology
- Insect and Pesticide Research
- Mitochondrial Function and Pathology
- Cholinesterase and Neurodegenerative Diseases
- Circadian rhythm and melatonin
- Technology and Human Factors in Education and Health
- Neural and Behavioral Psychology Studies
- Bee Products Chemical Analysis
- Regulation of Appetite and Obesity
- Transcranial Magnetic Stimulation Studies
University College London
2022-2025
Sainsbury Laboratory
2024-2025
NYU Langone Health
2021
Instituto Cajal
2015-2020
Consejo Superior de Investigaciones Científicas
2016
Fast instinctive responses to environmental stimuli can be crucial for survival but are not always optimal. Animals adapt their behavior and suppress reactions, the neural pathways mediating such ethologically relevant forms of learning remain unclear. We found that posterolateral higher visual areas (plHVAs) escapes from innate threats through a top-down pathway ventrolateral geniculate nucleus (vLGN). plHVAs no longer necessary after learning; instead, learned relies on plasticity within...
Interneurons are critical for proper neural network function and can activate Ca2+ signaling in astrocytes. However, the impact of interneuron-astrocyte into neuronal operation remains unknown. Using simplest hippocampal Astrocyte-Neuron network, i.e., GABAergic interneuron, pyramidal neuron, single CA3-CA1 glutamatergic synapse, astrocytes, we found that dynamically affected excitatory neurotransmission an activity- time-dependent manner, determined sign (inhibition vs potentiation)...
Astrocytes play crucial roles in brain homeostasis and are emerging as regulatory elements of neuronal synaptic physiology by responding to neurotransmitters with Ca2+ elevations releasing gliotransmitters that activate receptors. Aging involves astrocytic alterations, being considered risk factor for neurodegenerative diseases. Most evidence the astrocyte-neuron signaling is derived from studies young animals; however, features adult aging remain largely unknown. We have investigated...
Abstract Optogenetics has been widely expanded to enhance or suppress neuronal activity and it recently applied glial cells. Here, we have used a new approach based on selective expression of melanopsin, G‐protein‐coupled photopigment, in astrocytes trigger Ca 2+ signaling. Using the genetically encoded indicator GCaMP6f two‐photon imaging, show that melanopsin is both competent stimulate robust IP3‐dependent signals astrocyte fine processes, evoke an ATP/Adenosine‐dependent transient boost...
Brain activity requires a flux of glucose to active regions sustain increased metabolic demands. Insulin, the main regulator handling in body, has been traditionally considered not intervene this process. However, we now report that insulin modulates brain metabolism by acting on astrocytes concert with IGF-I. The cooperation and IGF-I is needed recover neuronal after hypoglycemia. Analysis underlying mechanisms show combined action synergistically stimulates mitogen-activated protein...
Abstract Interhemispheric axons of the corpus callosum (CC) facilitate higher order functions cerebral cortex. According to current views, callosal and non-callosal fates are determined early after a neuron’s birth, certain populations, such as cortical layer (L) 4 excitatory neurons primary somatosensory (S1) barrel, project only ipsilaterally. Using novel axonal-retrotracing strategy GFP-targeted visualization Rorb + neurons, we instead demonstrate that L4 develop transient...
Given the implication of histone acetylation in memory processes, deacetylase inhibitors (HDACIs) have been postulated as potential modulators cognitive impairment Alzheimer's disease (AD). However, dose-dependent side effects described patients with currently available broad-spectrum HDACIs, explaining why their therapeutic has not realized for chronic diseases. Here, by simultaneously targeting two independent enzyme activities, (HDAC) and phosphodiesterase-5 (PDE5), we propose a novel...
Fast instinctive responses to environmental stimuli can be crucial for survival, but are not always optimal. Based on prior experience, animals thus adapt their behavior and suppress reactions. However, the neural pathways mediating such ethologically relevant forms of learning remain unclear. We show that posterolateral higher visual areas (plHVAs) escapes from innate threats through a top-down pathway involving ventrolateral geniculate nucleus (vLGN). plHVAs no longer necessary after...
Subthalamic nucleus (STN) is the main source of feed-forward excitation in basal ganglia and a target therapeutic deep brain stimulation movement disorders. Alleviation motor symptoms during STN can be accompanied by deterioration abilities to quickly choose between conflicting alternatives. Cortical afferents subthalamic region (ST), comprising zona incerta (ZI), include projections from medial prefrontal cortex (mPFC), yet little known about prefrontal-subthalamic coordination its...
Here, we present a protocol to selectively downregulate GABAB receptor (GABABR) expression in astrocytes of mouse medial prefrontal cortex (mPFC). We first describe the procedure surgeries and viral injections. then detail genetic, histological, functional characterizations astrocytic GABABR ablation using RT-PCR, imaging, behavioral assays. The use flox mice can be easily adapted generate astrocyte-selective different brain areas postnatal stages, leading local downregulation...
Abstract Reducing insulin-like growth factor I receptor (IGF-IR) levels or administration of IGF-I show beneficial effects in the brain. We now provide evidence to help resolve this paradox. The unliganded IGF-IR inhibits glucose uptake by astrocytes while its stimulation with IGF-I, concert insulin activation receptor, produces opposite effect. In vivo imaging showed that shRNA interference brain increased pharmacological blockade reduced it. Brain 18 FGlucose-PET injected mice confirmed an...