A5011011292- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Alzheimer's disease research and treatments
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Histone Deacetylase Inhibitors Research
- Advanced biosensing and bioanalysis techniques
- Phosphodiesterase function and regulation
- Photochromic and Fluorescence Chemistry
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Neuroscience, Education and Cognitive Function
- Adenosine and Purinergic Signaling
- Tryptophan and brain disorders
- Receptor Mechanisms and Signaling
- Field-Flow Fractionation Techniques
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cannabis and Cannabinoid Research
- Biochemical effects in animals
- Graphene and Nanomaterials Applications
Instituto Cajal
2015-2024
Consejo Superior de Investigaciones Científicas
2009-2024
University of California, Berkeley
2019
University of California, San Francisco
2019
Massachusetts Institute of Technology
2012-2014
Astrocytes play active roles in brain physiology. They respond to neurotransmitters and modulate neuronal excitability synaptic function. However, the influence of astrocytes on transmission plasticity at single synapse level is unknown. Ca(2+) elevation transiently increased probability transmitter release hippocampal area CA3-CA1 synapses, without affecting amplitude events. This form short-term was due glutamate from astrocytes, a process that depended soluble N-ethylmaleimide-sensitive...
The synaptic control of the astrocytic intracellular Ca 2+ is crucial in reciprocal astrocyte-neuron communication. Using electrophysiological and imaging techniques rat hippocampal slices, we investigated signal modulation induced by terminals that use glutamate acetylcholine. elevations were evoked released from Schaffer collaterals acetylcholine, but not glutamate, alveus stimulation, indicating astrocytes discriminate activity different synapses belonging to axon pathways. was modulated...
Long-term potentiation (LTP) of synaptic transmission represents the cellular basis learning and memory. Astrocytes have been shown to regulate plasticity. However, their involvement in specific physiological processes that induce LTP vivo remains unknown. Here we show cholinergic activity evoked by sensory stimulation or electrical septal nucleus increases Ca2+ hippocampal astrocytes induces CA3-CA1 synapses, which requires muscarinic (mAChR) metabotropic glutamate receptor (mGluR)...
Astrocytes are important regulatory elements in brain function. They respond to neurotransmitters and release gliotransmitters that modulate synaptic transmission. However, the cell- synapse-specificity of functional relationship between astrocytes neurons certain circuits remains unknown. In dorsal striatum, which mainly comprises two intermingled subtypes (striatonigral striatopallidal) medium spiny (MSNs) synapses belonging neural (the direct indirect pathways basal ganglia),...
Astrocytes play important roles in synaptic transmission and plasticity. Despite vitro evidence, their causal contribution to cortical network activity sensory information processing vivo remains unresolved. Here we report that selective photostimulation of astrocytes with channelrhodopsin-2 primary visual cortex enhances both excitatory inhibitory transmission, through the activation type 1a metabotropic glutamate receptors. Photostimulation increases spontaneous firing parvalbumin-positive...
This work introduces NeoMag, a system designed to enhance cell mechanics assays in substrate deformation studies. NeoMag uses multidomain magneto-active materials mechanically actuate the substrate, transmitting reversible mechanical cues cells. The boasts full flexibility alternating loading modes, seamlessly adapting both upright and inverted microscopes. substrates facilitate mechanobiology on 2D 3D cultures. integration of with nanoindenters allows for precise evaluation cellular...
Recent results have demonstrated the existence of bidirectional communication between glial cells and neurons. We investigated in brain slices whether rat hippocampal astrocytes respond to acetylcholine synaptically released by an extrinsic pathway. stimulated stratum oriens/alveus, which contains cholinergic afferents from septum diagonal band Broca, recorded whole-cell membrane currents intracellular Ca 2+ levels located oriens. Nerve-fiber stimulation evoked a long-lasting inward current...
Abstract Astrocytes play a critical role in brain homeostasis controlling the local environment normal as well pathological conditions, such during hypoxic/ischemic insult. Since astrocytes have recently been identified source for wide variety of gliotransmitters that modulate synaptic activity, we investigated whether hypoxia‐induced excitatory depression might be mediated by adenosine release from astrocytes. We used electrophysiological and Ca 2+ imaging techniques hippocampal slices...
Although cholinergic innervation of the cortex by nucleus basalis (NB) is known to modulate cortical neuronal responses and instruct plasticity, little about underlying cellular mechanisms. Using cell-attached recordings in vivo, we demonstrate that electrical stimulation NB, paired with visual stimulation, can induce significant potentiation excitatory neurons primary mice. We further show vivo two-photon calcium imaging, ex whole-cell this pairing-induced mediated direct activation...
Interneurons are critical for proper neural network function and can activate Ca2+ signaling in astrocytes. However, the impact of interneuron-astrocyte into neuronal operation remains unknown. Using simplest hippocampal Astrocyte-Neuron network, i.e., GABAergic interneuron, pyramidal neuron, single CA3-CA1 glutamatergic synapse, astrocytes, we found that dynamically affected excitatory neurotransmission an activity- time-dependent manner, determined sign (inhibition vs potentiation)...
Brain function is recognized to rely on neuronal activity and signaling processes between neurons, whereas astrocytes are generally considered play supportive roles for proper function. However, accumulating evidence indicates that sense control synaptic activity, indicating neuron reciprocally communicate. While this has been obtained in experimental animal models, whether bidirectional neurons occurs human brain remains unknown. We have investigated the existence of astrocyte–neuron...
Endocannabinoids (eCBs) play key roles in brain function, acting as modulatory signals synaptic transmission and plasticity. They are recognized retrograde messengers that mediate long-term depression (LTD), but their ability to induce potentiation (LTP) is poorly known. We show eCBs the enhancement of transmitter release at single hippocampal synapses through stimulation astrocytes when coincident with postsynaptic activity. This LTP requires coordinated activity 3 elements tripartite...
Astrocytes exhibit spontaneous calcium oscillations that could induce the release of glutamate as gliotransmitter in rat hippocampal slices. However, it is unknown whether this astrocytic may contribute to determining basal neurotransmitter probability central synapses. Using whole-cell recordings and Ca2+ imaging, we investigated effects activity on neurotransmission synaptic plasticity at CA3–CA1 We show here metabolic gliotoxin fluorocitrate (FC) reduces amplitude evoked excitatory...
Astrocytes play crucial roles in brain homeostasis and are emerging as regulatory elements of neuronal synaptic physiology by responding to neurotransmitters with Ca2+ elevations releasing gliotransmitters that activate receptors. Aging involves astrocytic alterations, being considered risk factor for neurodegenerative diseases. Most evidence the astrocyte-neuron signaling is derived from studies young animals; however, features adult aging remain largely unknown. We have investigated...
Abstract Optogenetics has been widely expanded to enhance or suppress neuronal activity and it recently applied glial cells. Here, we have used a new approach based on selective expression of melanopsin, G‐protein‐coupled photopigment, in astrocytes trigger Ca 2+ signaling. Using the genetically encoded indicator GCaMP6f two‐photon imaging, show that melanopsin is both competent stimulate robust IP3‐dependent signals astrocyte fine processes, evoke an ATP/Adenosine‐dependent transient boost...
Astrocytes play crucial roles in brain homeostasis and are regulatory elements of neuronal synaptic physiology. Astrocytic alterations have been found Major Depressive Disorder (MDD) patients; however, the consequences astrocyte Ca2+ signaling MDD poorly understood. Here, we that corticosterone-treated juvenile mice (Cort-mice) showed altered astrocytic dynamics mPFC both resting conditions during social interactions, line with behavior. Additionally, Cort-mice displayed reduced serotonin...