A5087622367- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- Genetically Modified Organisms Research
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- Click Chemistry and Applications
- Fungal and yeast genetics research
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Protist diversity and phylogeny
- Computational Drug Discovery Methods
- Genetic and Kidney Cyst Diseases
- Mitochondrial Function and Pathology
- Protein Tyrosine Phosphatases
Lunenfeld-Tanenbaum Research Institute
2013-2022
Sinai Health System
2018-2022
Mount Sinai Hospital
2013-2022
University of Toronto
2012
Highlights•The phosphatase interactome contains 1,335 interactions made by 140 phosphatases•Mitosis requires phosphatases of all evolutionary lineages, including several PTPs•PTPs play diverse roles in mitosis, notably adhesion and centriole biogenesis•Combining complementary screens provides testable hypotheses about protein functionSummaryReversible phosphorylation is a fundamental regulatory mechanism, intricately coordinated kinases phosphatases, two classes enzymes widely disrupted...
SummaryAutophagy controls cellular catabolism in diverse eukaryotes and modulates programmed cell death plants animals. While studies of the unicellular yeast Saccharomyces cerevisiae have provided fundamental insights into mechanisms autophagy, roles pathways are less well understood. Here, we describe widespread developmentally nuclear destruction (PND) events that occur during gametogenesis. PND is executed through apoptotic-like DNA fragmentation coordination with an unusual form...
ABSTRACT Compartmentalization is a defining characteristic of eukaryotic cells, partitioning cellular processes into discrete subcellular locations. High throughput microscopy 1 and biochemical fractionation coupled with mass spectrometry 2–6 have helped to define the proteomes variety organelles macromolecular structures. However, many other intracellular compartments remained refractory such approaches, due for example difficulty in purifying non-membrane bound Proximity-dependent...
Centrosomes are required for faithful chromosome segregation during mitosis. They composed of a centriole pair that recruits and organizes the microtubule-nucleating pericentriolar material. Centriole duplication is tightly controlled in vivo aberrations this process associated with several human diseases, including cancer microcephaly. Although factors essential assembly, such as STIL PLK4, have been identified, underlying molecular mechanisms drive incompletely understood. Combining...
Studies of the budding yeast Saccharomyces cerevisiae have provided many most important insights into mechanisms autophagy, which are common to all eukaryotes. However, investigation self-destruction pathways, including autophagy and programmed cell death, has been almost exclusively restricted cells undergoing vegetative growth, leaving very little exploration their functions during developmental transitions in life cycle. We recently discovered that whole nuclei subject destruction...