A5075295489- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Renal and related cancers
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Nuclear Structure and Function
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Effects and risks of endocrine disrupting chemicals
- Genomics and Chromatin Dynamics
- Retinoids in leukemia and cellular processes
- Cancer, Lipids, and Metabolism
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- interferon and immune responses
- Multiple Myeloma Research and Treatments
- Phagocytosis and Immune Regulation
Huazhong Agricultural University
2015-2024
National Institute of Diabetes and Digestive and Kidney Diseases
2015-2024
National Institutes of Health
2015-2024
First Affiliated Hospital of Xi'an Jiaotong University
2008-2024
Nantong University
2021-2024
Harbin Medical University
2021-2024
University of Oxford
2018-2024
University of Illinois Urbana-Champaign
2024
National Cancer Institute
2024
Shanghai Zhangjiang Laboratory
2024
Summary Hutchinson–Gilford progeria syndrome ( HGPS ), a fatal premature aging disease, is caused by single‐nucleotide mutation in the LMNA gene. Previous reports have focused on nuclear phenotypes cells, yet potential contribution of mitochondria, key player normal aging, remains unclear. Using high‐resolution microscopy analysis, we demonstrated significantly increased fraction swollen and fragmented mitochondria marked reduction mitochondrial mobility fibroblast cells. Notably, expression...
Centrosomes are composed of a centriolar core surrounded by pericentriolar material (PCM) matrix that docks microtubule-nucleating γ-tubulin complexes. During mitotic entry, the PCM increases in size and nucleating capacity process called centrosome maturation. Polo-like kinase 1 (PLK1) is recruited to centrosomes phosphorylates proteins drive their self-assembly, which leads expansion. Here, we show addition controlling expansion, PLK1 independently controls generation binding sites for...
Significance The G9a histone methyltransferase primarily regulates the expression of genes associated with cancer development in cells, but it has also been implicated mediating DNA damage response. Here, we confirmed a role for repair following double-strand breaks. is recruited to chromatin as result casein kinase 2-mediated phosphorylation, where directly interacts replication protein A (RPA). binding RPA modulates and Rad51 foci formation permits efficient homologous recombination. This...
Alternative splicing contributes to phenotypic diversity at multiple biological scales, and its dysregulation is implicated in both ageing age-associated diseases human. Cross-tissue variability further complicates links phenotypes elucidating these requires a comprehensive map of changes across tissues. Here, we generate such by analyzing ~8500 RNA-seq samples 48 tissues 544 individuals. Employing stringent model controlling for confounders, identify 49,869 tissue-specific events 7 distinct...
Abstract Background Recurrent bladder cancer is the most common type of urinary tract malignancy; nevertheless, mechanistic basis for its recurrence uncertain. Innovative technologies such as single‐cell transcriptomics and spatial (ST) offer new avenues studying recurrent tumour progression at level while preserving data. Method This study integrated RNA (scRNA) sequencing ST profiling to examine microenvironment (TME) six tissues (three from primary tumours three tumours). Findings scRNA...
Fragile X-associated disorders are Repeat Expansion Diseases that result from expansion of a CGG/CCG-repeat in the FMR1 gene. Contractions repeat tract also occur, albeit at lower frequency. However, these contractions can potentially modulate disease symptoms or generate an allele with numbers normal range. Little is known about mechanism and even less contractions. We have previously demonstrated mismatch repair (MMR) protein MSH2 required for expansions mouse model disorders. Here, we...
Lamin A (LA) is a critical structural component of the nuclear lamina. Mutations within LA gene (LMNA) lead to several human disorders, most striking which Hutchinson-Gilford Progeria Syndrome (HGPS), premature aging disorder. HGPS cells are best characterized by an abnormal morphology known as blebbing, arises due accumulation progerin, dominant mutant form LA. The microtubule (MT) network mediate changes in context specific events such mitosis, cell polarization, nucleus positioning and...
Growing mammalian oocytes accumulate substantial amounts of RNA, most which is degraded during subsequent meiotic maturation. The growth-to-maturation transition begins with germinal vesicle or nuclear envelope breakdown (GVBD) and critical for oocyte quality early development. molecular machinery responsible the transcriptome remains unclear. Here, we report that an exosome-associated RNase, EXOSC10, sculpts to facilitate mouse oocytes. We establish oocyte-specific conditional knockout...
Abstract Objectives To explore the correlations among social isolation and symptoms of anxiety depression patients with breast cancer in China to further verify mediating role support or anxiety. Design A cross‐sectional survey. Settings The cluster sampling method was conducted for 456 female inpatients diagnosed at Tumor Hospital Affiliated Harbin Medical University from April 2019–September 2019. Methods Pearson correlation analysis used identifying all variables. Mediation effect examine...
Translation of maternal mRNAs is detected before transcription zygotic genes and essential for mammalian embryo development. How certain are selected translation instead degradation how this burst affects genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic initiation factor 4E family member 1b (eIF4E1b) regulator mRNA expression ensures subsequent reprogramming genome. In oocytes, eIF4E1b binds to transcripts encoding machinery proteins,...
Lipodystrophies, characterized by partial or complete loss of adipose tissue, have been associated with mutations in the lamin A gene. It remains unclear how mutants interfere tissue formation. HutchinsonâGilford progeria syndrome (HGPS) presents most severe form A-associated diseases, whose patients show a subcutaneous fat. Using iPSCs reprogrammed from HGPS fibroblasts, we induced adipocyte formation iPSC derived embryoid bodies mesenchymal stem cells. Both approaches revealed lipid...
Fluorene‐9‐bisphenol (BHPF), a substitute of bisphenol A (BPA) used in the production so‐called “BPA‐free” plastics, has now been shown to be released from commercial plastic bottles into drinking water and strong anti‐estrogenic activity mice, which suggests that BHPF is also an environmental toxin. However, whether exposure effects on mouse oocyte development unknown. In this study, influence acute (50–150 μM, 12 hr) maturation its possible mechanisms were investigated. Of note, 50‐μM had...
Centrosomes are required for faithful chromosome segregation during mitosis. They composed of a centriole pair that recruits and organizes the microtubule-nucleating pericentriolar material. Centriole duplication is tightly controlled in vivo aberrations this process associated with several human diseases, including cancer microcephaly. Although factors essential assembly, such as STIL PLK4, have been identified, underlying molecular mechanisms drive incompletely understood. Combining...
Fluorene-9-bisphenol (9,9-bis(4-hydroxyphenyl)-fluorene [BHPF]) is a bisphenol A (BPA) substitute used in the production of "BPA-free" plastics, now has been identified harmful to living organisms. Our previous study showed that BHPF impaired mouse denuded oocyte vitro maturation. However, there question whether still able affect maturation presence dense cumulus cells. In present study, we checked toxic effects on porcine which derived from COCs culture. results (50 μM) inhibited expansion...
Highlights•Lyssavirus inhibits NLRP3 inflammasome activation•Lyssavirus M protein competes with NEK7 for binding to NLRP3•The lyssavirus S158 site is essential inhibition•Lyssavirus M-NLRP3 interaction keeps in a locked stateSummaryLyssavirus kind of neurotropic pathogen that needs evade peripheral host immunity enter the central nervous system accomplish infection. activation defend against invasion. This study demonstrates matrix (M) can inhibit both priming step and activation....
Cooperative communications between the central spindle and contractile ring are critical for spatial temporal regulation of cytokinesis. Here we report that MyoGEF, a guanine nucleotide exchange factor localizes to cleavage furrow, interacts with centrosome/spindle pole-associated protein (CSPP), which is concentrated at pole during mitosis Both in vitro vivo pulldown assays show MyoGEF CSPP. The C-terminus N-terminus CSPP required their interaction. Immunofluorescence analysis indicates...
As a member of parabens (PBs), Isobutylparaben (IBP) has broad-spectrum antimicrobial activity and widely used in personal care products cosmetics. Recent studies have indicated that usage IBP poses potential threat to reproductive health. In this study, we aimed reveal the effects acute exposure on meiotic maturation porcine cumulus oocyte complexes. Initial study showed 200 μM significantly reduced rate first polar body extrusion with no significant effect cell expansion; however, 400...
The epidemiological studies regarding perfluorooctanoic acid (PFOA) suggests that its exposure causes reproductive health issues, the underlying mechanisms of which are still in infancy. Here, we report PFOA deteriorates female reproduction at multiple development stages. Oocyte meiosis and preimplantation severely impaired by with oxidative stress being a contributor. Supplementing antioxidant melatonin partially rescues oocyte meiotic maturation non-apoptotic demise. attenuation ovarian...