A5047360142- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Plant tissue culture and regeneration
- Connective tissue disorders research
- Protease and Inhibitor Mechanisms
- Genetically Modified Organisms Research
- CRISPR and Genetic Engineering
- Lipoproteins and Cardiovascular Health
- Plant and animal studies
- Insect Resistance and Genetics
- Biological Stains and Phytochemicals
- Postharvest Quality and Shelf Life Management
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Bacterial biofilms and quorum sensing
- Connective Tissue Growth Factor Research
- Collagen: Extraction and Characterization
- Transgenic Plants and Applications
- Pineapple and bromelain studies
- Horticultural and Viticultural Research
- Phytochemicals and Antioxidant Activities
- Plant Genetic and Mutation Studies
- Tissue Engineering and Regenerative Medicine
- Biomarkers in Disease Mechanisms
Universidade Federal de Santa Catarina
2010-2022
Aomori Prefectural Industrial Technology Center
2010
Case Western Reserve University
1981-2005
Western University of Health Sciences
2001
University Hospitals of Cleveland
1994-1999
University School
1981-1999
Cleveland Clinic
1975-1987
Cleveland Foundation
1975-1986
Enzymatic browning is one of the most important reactions that occur in fruits and vegetables, usually resulting negative effects on color, taste, flavor, nutritional value. The reaction a consequence phenolic compounds' oxidation by polyphenol oxidase (PPO), which triggers generation dark pigments. This particularly relevant for apples, are rich polyphenols highly susceptible to enzymatic browning. objective present work was quantify PPO activity identify target apple [ Malus × sylvestris...
Collagen binding was examined in 90 strains of Staphylococcus aureus derived from patient samples. Slightly under one-half (39 90) the S. bound collagen. did not correlate with either immunoglobulin G or fibronectin by these organisms. Chi-square analysis isolates obtained patients complicated bacteremia (bacteremia associated deep tissue infection) compared uncomplicated without other showed that former were significantly more likely to have collagen-binding ability. Subcloning primary all...
Staphylococcus aureus can bind soluble collagen in a specific, saturable manner. We have previously shown that some variability exists the degree of binding between different strains heat-killed, formaldehyde-fixed S. which are commercially available as immunologic reagents. The present study demonstrates live Cowan 1 strain binds amounts per organism equivalent to those demonstrated bacteria but has an affinity over 100 times greater, with Kd values 9.7 X 10(-11) M and 4.3 10(-8) for...
Insect resistance is the second most common trait globally in cultivated genetically modified (GM) plants. Resistance usually obtained by introducing into plant’s genome genes from bacterium Bacillus thuringiensis (Bt) coding for insecticidal proteins (Cry or toxins) that target insect pests. The aim of this study was to examine hypothesis a chronic, high-dose dietary exposure leaves Bt–maize hybrid (GM event MON810, expressing transgenic recombinant Cry1Ab toxin), exerted no adverse effects...
Objective The Arg519–Cys mutation in type II collagen results severe, precocious familial osteoarthritis (OA) 100% of carriers within the first 3 decades life. carrier population provided a well-defined patient for study serum markers OA with respect to pathogenesis, diagnosis, and prognosis. Methods Serum was obtained from 31 mutation-positive individuals 16 mutation-negative individuals. severity determined by clinical radiologic assessments. Levels cartilage oligomeric matrix protein...
Abstract Objective . To investigate the occurrence and clinical correlation of arg 519 ‐to‐cys mutation in type II procollagen gene patients with osteoarthritis (OA). Methods Sixty‐six subjects from 7 families a strong family history generalized OA 13 erosive were evaluated clinically radiologically. Blood samples 58 familial group all obtained for DNA analysis. Exon 31 COL2A1, which spans residue 519, was amplified by polymerase chain reaction. Results The detected 2 OA. In these families,...
Acca sellowiana (Berg) Burret is a predominantly allogamous species with hermaphrodite flowers that has barriers to self-fertilisation such as dichogamy by protogyny and self-incompatibility. This study aimed identify when self-incompatibility occurs in A. submitted self-pollinations. Pollinations were made using nine known self-incompatible accessions several treatments including manual cross-pollination, self-pollination, no pollination natural pollination. Flowers pollinated pistils...
Herbicide tolerant plants such as Roundup-Ready soybean contain residues of glyphosate herbicide. These are considered safe and previous animal-feeding-studies have failed to find negative effects related chemical residues. The present study tests 8 experimental soy- meal diets feed in groups (each containing 20 individuals) test-animals (D. magna). different levels we show that animal growth, reproductive maturity number offspring correlated with these chemicals. tested soybeans from...
The induction and subsequent regeneration of protocorm-like bodies (PLB) is the most common morphogenetic pathway for orchid micropropagation. plants from PLB can be a bottleneck some species due to asynchronicity. Genetic fidelity another issue, cytogenetic stability regenerated plantlets deserves special attention occurrence endopolyploidy in orchids. In present work, we defined optimal conditions plant Cattleya tigrina, threatened Brazilian Atlantic Rain Forest. We estimated optimum...
Collagen, fibronectin, and nonfibrous protein biosynthesis were examined in cultures of rabbit arterial smooth muscle cells grown on tissue culture plastic precoated either with plasma fibronectin or bovine serum albumin. Cells seeded into fibronectin-coated wells appeared to reach confluence more quickly than counterparts albumin-coated surfaces. Measurement 3H-thymidine incorporation DNA by these suggested that this was probably a consequence rapid efficient cell attachment rather an...
Arginine519-cysteine mutation in the type II procollagen gene (COL2A1) is known to be associated with mild spondyloepiphyseal dysplasia (SED) and precocious generalized osteoarthritis (OA). Five families have now been identified this mutation. To determine whether a common founder was responsible for these five families, we defined haplotype of mutation-bearing chromosome using four restriction fragment length polymorphisms (RFLPs) 3′-untranslated region VNTR. Haplotype frequencies were...
Micropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established: immersion system (2 L twin flasks temporary permanent immersion); culture medium (supplemented or not GA3); shoot height class. The effects on regeneration rate (number new shoots per 1 g fresh (FM) inoculated microshoots), FM shoot, survival were...
SummaryCollagen synthesis was measured in segments of normal rabbit aorta, incubated vitro, by monitoring the formation peptidyl-14C-hydroxyproline from [U-14C]-L-proline added to incubation medium. The effect hyperlipoproteinemic serum on rate collagen compared with serum. No differences rates were detected between two batches serum, despite a 60-fold difference cholesterol concentration.Increases free proline concentration medium resulted changes flux and tissue pools proline, but had no synthesis.