A5063609159- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Neurogenetic and Muscular Disorders Research
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neuropharmacology Research
- Genetics, Aging, and Longevity in Model Organisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Amyotrophic Lateral Sclerosis Research
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Bacteriophages and microbial interactions
- Aluminum toxicity and tolerance in plants and animals
- Lysosomal Storage Disorders Research
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Medicinal Plant Pharmacodynamics Research
- Cholinesterase and Neurodegenerative Diseases
Institute of Biological Chemistry, Academia Sinica
2017-2024
National Taiwan University
2017-2024
Academia Sinica
2017-2021
National Yang Ming Chiao Tung University
2005-2015
Institute of Molecular Biology, Academia Sinica
2005-2015
Baylor College of Medicine
2009-2014
Howard Hughes Medical Institute
2008-2009
Mitochondrial fusion and fission affect the distribution quality control of mitochondria. We show that Marf (Mitochondrial associated regulatory factor), is required for mitochondrial transport in long axons. Moreover, loss leads to a severe depletion mitochondria neuromuscular junctions (NMJs). mutants also fail maintain proper synaptic transmission at NMJs upon repetitive stimulation, similar Drp1 mutants. However, unlike Drp1, NMJ morphology defects extended larval lifespan. form contacts...
Abstract Changes in mitochondrial dynamics (fusion and fission) are known to occur during stem cell differentiation; however, the role of this phenomenon tissue aging remains unclear. Here, we report that shifted toward fission Drosophila ovarian germline cells (GSCs), shift contributes aging‐related GSC loss. We found as GSCs age, fragmentation expression regulator, Dynamin‐related protein (Drp1), both increased, while membrane potential is reduced. Moreover, preventing fusion results...
Overproduction of reactive oxygen species (ROS) is known to mediate glutamate excitotoxicity in neurological diseases. However, how ROS burdens can influence neural circuit integrity remains unclear. Here, we investigate the impact induced by depletion Drosophila Eaat1, an astrocytic transporter, on locomotor central pattern generator (CPG) activity, neuromuscular junction architecture, and motor function. We show that triggers a circuit-dependent feedback loop sculpt system. Excitotoxicity...
In a screen to identify genes involved in synaptic function, we isolated mutations Drosophila melanogaster straightjacket (stj), an α2δ subunit of the voltage-gated calcium channel. stj mutant photoreceptors develop normal connections but display reduced “on–off” transients electroretinogram recordings, indicating failure evoke postsynaptic responses and, thus, defect neurotransmission. is expressed neurons excluded from glia. Mutants exhibit endogenous seizure-like activity, altered...
Clathrin-mediated endocytosis (CME) and activity-dependent bulk (ADBE) are two predominant forms of synaptic vesicle (SV) endocytosis, elicited by moderate strong stimuli, respectively. They tightly coupled with exocytosis for sustained neurotransmission. However, the underlying mechanisms ill defined. We previously reported that Flower (Fwe) Ca2+ channel present in SVs is incorporated into periactive zone upon SV fusion, where it triggers CME, thus coupling to CME. Here, we show Fwe also...
Neuromuscular junctions (NMJs) govern efficient neuronal communication with muscle cells, relying on proper architecture of specialized postsynaptic compartments. However, the intrinsic mechanism in cells contributing to NMJ development remains unclear. In this study, we reveal that dynamin-2 (Dyn2) is involved NMJs. Mutations Dyn2 have been linked human muscular disorder and centronuclear myopathy (CNM), as well featured atrophy defective NMJs, yet function at membrane largely unknown. We...
Hexanucleotide repeat expansion in C9ORF72 ( C9 ) is the most prevalent mutation among amyotrophic lateral sclerosis (ALS) patients. The patients carry over ~30 to hundreds or thousands of repeats translated dipeptide (DPRs) where poly-glycine-arginine (GR) and poly-proline-arginine (PR) are toxic. structure-function relationship still unknown. Here, we examined minimal neurotoxic number poly-GR found that extension led a loose helical structure disrupting plasma nuclear membrane. Poly-GR/PR...
Modulation of presynaptic actin dynamics is fundamental to synaptic growth and functional plasticity; yet the underlying molecular cellular mechanisms remain largely unknown. At Drosophila NMJs, Rac1-SCAR pathway mediates BMP-induced receptor macropinocytosis inhibit BMP signaling. Here, we show that Rho-type GEF Vav acts upstream Rac1 through macropinocytosis. We also present evidence Vav-Rac1-SCAR signaling has additional roles in tetanus-induced plasticity. Presynaptic inactivation...
Synaptic vesicle (SV) endocytosis is coupled to exocytosis maintain SV pool size and thus neurotransmitter release. Intense stimulation induces activity-dependent bulk (ADBE) recapture large quantities of constituents in endosomes from which SVs reform. How these consecutive processes are spatiotemporally coordinated remains unknown. Here, we show that Flower Ca 2+ channel-dependent phosphatidylinositol 4,5-bisphosphate (PI(4,5)P 2 ) compartmentalization governs control Drosophila . Strong...
Mutations in the human ALS2 gene cause recessive juvenile-onset amyotrophic lateral sclerosis and related motor neuron diseases. Although protein has been identified as a guanine-nucleotide exchange factor for small GTPase Rab5, its physiological roles remain largely unknown. Here, we demonstrate that Drosophila homologue of (dALS2) promotes postsynaptic development by activating Frizzled nuclear import (FNI) pathway. dALS2 loss causes structural defects subsynaptic reticulum (SSR),...
Organ formation requires a delicate balance of positive and negative regulators. In Drosophila eye development, wingless (wg) is expressed at the lateral margins disc serves to block retinal development. The T-box gene optomotor-blind (omb) in similar pattern regulated by Wg. Omb mediates part Wg activity blocking exerts its function primarily cell proliferation. These effects occur predominantly ventral margin. Our results suggest that primary effect Jak/STAT signaling repressing...
This protocol describes the generation of mFwe Knockout n2a cells. It is from 'Flower Ca2+ channel in CME and ADBE' Yao CK et al. Please see manuscript for full method details.
This is a collection of protocols from 'Flower Ca2+ channel in CME and ADBE' Yao CK et al. (2017). Please see the manuscript for full method details. References: Haucke V, Neher E, Sigrist SJ (2011) Protein scaffolds coupling synaptic exocytosis endocytosis. Nat Rev Neurosci 12: 127-138. Wu LG, Hamid Shin W, Chiang HC (2014) Exocytosis endocytosis: modes, functions, mechanisms. Annu Physiol 76: 301-331. Kononenko NL, V (2015) Molecular mechanisms presynaptic membrane retrieval vesicle...
This protocol is from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for full method details.
This protocol describes the RT-PCR from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see manuscript for full method details.
This protocol describes the immunostaining of cultured rat hippocampal neurons to stain for ratFwe2 in cell body. It is from 'Flower Ca2+ channel CME and ADBE' Yao CK et al. Please see manuscript full method details.
This protocol describes the Immunostaining in fly NMJ boutons. It is from 'Flower Ca2+ channel CME and ADBE' of Yao CK et al. Please see manuscript for full method details.
This protocol is from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for full method details.