A5000062611- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Genetic Neurodegenerative Diseases
- Renal and related cancers
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Retinal Development and Disorders
- Advanced biosensing and bioanalysis techniques
- Autism Spectrum Disorder Research
- Lysosomal Storage Disorders Research
- Neurogenetic and Muscular Disorders Research
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Amyotrophic Lateral Sclerosis Research
- Nuclear Receptors and Signaling
- biodegradable polymer synthesis and properties
- RNA modifications and cancer
Genomics Research Center, Academia Sinica
2010-2024
Institute of Cellular and Organismic Biology, Academia Sinica
2013-2017
Czech Academy of Sciences, Institute of Biotechnology
2012
Genomics (United Kingdom)
2012
Trans -splicing is a post-transcriptional event that joins exons from separate pre-mRNAs. Detection of trans usually severely hampered by experimental artifacts and genetic rearrangements. Here, we develop new computational pipeline, TSscan, which integrates different types high-throughput long-/short-read transcriptome sequencing human embryonic stem cell (hESC) lines to effectively minimize false positives while detecting -splicing. Combining TSscan screening with multiple validation steps...
Huntington's disease (HD) is an autosomal-dominant degenerative caused by a cytosine-adenine-guanine trinucleotide expansion in the Huntingtin (htt) gene. The most vulnerable brain areas to mutant HTT-evoked toxicity are striatum and cortex. In spite of extensive efforts that have been devoted characterization HD pathogenesis, no disease-modifying therapy for currently available. A2A adenosine receptor (A2AR) widely distributed brain, with highest level observed striatum. We previously...
The mechanisms of transcriptional regulation underlying human primordial germ cell (PGC) differentiation are largely unknown. repressor Prdm1/Blimp-1 is known to play a critical role in controlling specification mice. Here, we show that PRDM1 expressed developing gonads and contributes the determination germline versus neural fate early development. We knockdown embryonic stem cells (hESCs) impairs potential upregulates genes. Conversely, ectopic expression hESCs promotes generation exhibit...
Spinocerebellar ataxias 2 and 3 (SCA2 SCA3) are dominantly inherited neurodegenerative diseases caused by expansion of polyglutamine-encoding CAG repeats in the affected genes. The etiology these disorders is known to involve widespread loss neuronal cells cerebellum, however, mechanisms that contribute cell death still elusive. Here we established SCA2 SCA3 induced pluripotent stem (iPSCs) demonstrated SCA-associated pathological features can be recapitulated SCA-iPSC-derived neurons....
Abstract Induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs) are a promising source of tailor-made cell therapy for neurological diseases. However, major obstacles to clinical use still exist. To circumvent complications related intracerebral administration, we implanted human iPSC-NPCs epidurally over the peri-infarct cortex 7 days after permanent middle cerebral artery occlusion in adult rats. Compared controls, cell-treated rats showed significant improvements...
Hexanucleotide repeat expansion in C9ORF72 ( C9 ) is the most prevalent mutation among amyotrophic lateral sclerosis (ALS) patients. The patients carry over ~30 to hundreds or thousands of repeats translated dipeptide (DPRs) where poly-glycine-arginine (GR) and poly-proline-arginine (PR) are toxic. structure-function relationship still unknown. Here, we examined minimal neurotoxic number poly-GR found that extension led a loose helical structure disrupting plasma nuclear membrane. Poly-GR/PR...
The future clinical use of embryonic stem cell (ESC)-based hepatocyte replacement therapy depends on the development an efficient procedure for differentiation hepatocytes from ESCs. Here we report that a high density human ESC-derived fibroblast-like cells (hESdFs) supported generation hepatocyte-like with functional and mature hepatic phenotypes primate ESCs induced pluripotent cells. Molecular immunocytochemistry analyses revealed hESdFs caused rapid loss pluripotency sequential...
Human embryonic stem cells (hESCs) have the capacity for self-renewal and multilineage differentiation, which are of clinical importance regeneration medicine. Despite significant progress hESC study, complete proteome atlas, especially surface protein composition, awaits delineation. According to latest release neXtProt database (January 17, 2018; 19 658 PE1, 2, 3, 4 human proteins), membrane proteins present major category (1047; 48%) among all 2186 missing (MPs). We conducted a deep...
Pompe disease (OMIM # 232300) is a glycogen storage caused by autosomal recessive mutations of the gene encoding alpha-1,4-glucosidase (GAA; EC 3.2.1.20). Despite relatively effective employment enzyme replacement therapy, some critical medical issues still exist in patients with this disease, including persistence abnormalities central nervous system (CNS), probably because inability recombinant GAA to pass through blood-brain barrier. To address issue, identification more therapeutic...