A5090250205- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Wnt/β-catenin signaling in development and cancer
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Prion Diseases and Protein Misfolding
- Cancer Genomics and Diagnostics
- Genetics, Aging, and Longevity in Model Organisms
- Hedgehog Signaling Pathway Studies
- Neurogenesis and neuroplasticity mechanisms
- Invertebrate Immune Response Mechanisms
- Colorectal Cancer Treatments and Studies
- Chromosomal and Genetic Variations
- Genomics and Rare Diseases
- Receptor Mechanisms and Signaling
- Cancer-related gene regulation
- Chemokine receptors and signaling
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Gastric Cancer Management and Outcomes
Duke-NUS Medical School
2023-2024
Institute of Cellular and Organismic Biology, Academia Sinica
2015-2022
National Taipei University
2017-2020
Academia Sinica
2017-2020
National Chung Hsing University
2017-2020
National Yang Ming Chiao Tung University
2013-2019
National Chiayi University
2015
Fujian Provincial Hospital
2015
Taipei Veterans General Hospital
2013
Abstract Changes in mitochondrial dynamics (fusion and fission) are known to occur during stem cell differentiation; however, the role of this phenomenon tissue aging remains unclear. Here, we report that shifted toward fission Drosophila ovarian germline cells (GSCs), shift contributes aging‐related GSC loss. We found as GSCs age, fragmentation expression regulator, Dynamin‐related protein (Drp1), both increased, while membrane potential is reduced. Moreover, preventing fusion results...
Stem cells require different types of supporting cells, or niches, to control stem cell maintenance and differentiation. However, little is known about how those niches are formed. We report that in the development Drosophila melanogaster ovary, Hedgehog (Hh) gradient sets differential affinity for somatic gonadal precursors specify stromal intermingled which contributes both germline differentiation adult. also Traffic Jam (an orthologue a large Maf transcription factor mammals) novel...
The ability of stem cells to switch between quiescent and proliferative states is crucial for maintaining tissue homeostasis regeneration. In Drosophila, neural (qNSCs) extend a primary protrusion, hallmark qNSCs. Here, we have found that qNSC protrusions can be regenerated upon injury. This regeneration process relies on the Golgi apparatus acts as major acentrosomal microtubule-organizing center in A Golgi-resident GTPase Arf1 its guanine nucleotide exchange factor Sec71 promote NSC...
ABSTRACT Diet is an important regulator of stem cell homeostasis; however, the underlying mechanisms this regulation are not fully known. Here, we report that insulin signaling mediates dietary maintenance Drosophila ovarian germline cells (GSCs) by promoting extension niche escort (EC) membranes to wrap around GSCs. This wrapping may facilitate delivery bone morphogenetic protein stemness factors from ECs in In addition effects on GSCs, signaling-mediated EC number and protrusions controls...
Abstract Transposons are known to participate in tissue aging, but their effects on aged stem cells remain unclear. Here, we report that the Drosophila ovarian germline cell (GSC) niche, aging-related reductions expression of Piwi (a transposon silencer) derepress retrotransposons and cause GSC loss. Suppression young niche mimics causing retrotransposon depression coincident activation Toll-mediated signaling, which promotes Glycogen synthase kinase 3 activity degrade β-catenin. Disruption...
The transitioning of neural stem cells (NSCs) between quiescent and proliferative states is fundamental for brain development homeostasis. Defects in NSC reactivation are associated with neurodevelopmental disorders.
In developing organisms, proper tuning of the number stem cells within a niche is critical for maintenance adult tissues; however, involved mechanisms remain largely unclear. Here, we demonstrate that Thickveins (Tkv), type I bone morphogenetic protein (BMP) receptor, acts in Drosophila ovarian soma through Smad-independent pathway to shape distribution BMP signal niche, impacting germline cell (GSC) recruitment and maintenance. Somatic Tkv promotes Egfr signaling silence transcription...
Therapeutic responses of lung adenocarcinoma patients to tyrosine kinase inhibitors (TKIs) epidermal growth factor receptor (EGFR) are closely associated with activating mutations within the EGFR domain. Screening prior selection for therapeutic strategy has been considered extremely valuable clinical management in Asian countries including Taiwan, where mutation rate is higher than rest world. Currently there no consensus on method choice assess tumour tissue.We enrolled 445 analysis using...
ADP ribosylation factor-like GTPase 2 (Arl2) is crucial for controlling mitochondrial fusion and microtubule assembly in various organisms. Arl2 regulates the asymmetric division of neural stem cells Drosophila via growth. However, function mammalian during cortical development was unknown. Here, we demonstrate that mouse plays a new role corticogenesis regulating growth, but not mitochondria functions. knockdown (KD) leads to impaired proliferation progenitor (NPCs) neuronal migration. KD...
Wnt/β-catenin signaling controls various cell fates in metazoan development, and its dysregulation is often associated with cancer formation. However, regulations of this pathway are not completely understood. Here, we report that Lzap, a tumor suppressor, nuclear translocation β-catenin. In zebrafish embryos disruption lzap increases the expression chordin (chd), which encodes bone morphogenetic protein (BMP) antagonist localized prospective dorsal cells promotes fates. Consistently,...
The aims of this study were to observe the differential expression PI3K-AKT pathway-related genes in seizure-inducing brain lesions type II focal cortical dysplasia, and explore relationship between gene histological changes dysplastic foci their epileptogenic mechanism.Typical tissue from three patients with epilepsy induced by dysplasia selected for analysis, along normal two control group individuals.Following quantitative analysis using RT2 Profiler TM PCR Array, pathway related was...
Adult stem cells maintain tissue homeostasis. This unique capability largely depends on the cell niche, a specialized microenvironment, which preserves identity through physical contacts and secreted factors. In many cancers, latent tumor niches are thought to house aid initiation. However, in developing cancer it is unclear how niche established. The well-characterized germline (GSCs) Drosophila melanogaster ovary provide an excellent model address this fundamental issue. As such, we...
ADP ribosylation factor-like GTPase 2 (Arl2) is crucial for controlling mitochondrial fusion and microtubule assembly in various organisms. Arl2 regulates the asymmetric division of neural stem cells Drosophila via growth. However, function mammalian during cortical development was unknown. Here, we demonstrate that mouse plays a new role corticogenesis regulating growth, but not mitochondria functions. knockdown leads to impaired proliferation progenitor (NPCs) neuronal migration. NPCs...
Abstract The transitioning of neural stem cells (NSCs) between quiescent and proliferative states is fundamental for brain development homeostasis. Defects in NSC reactivation are associated with neurodevelopmental disorders. Drosophila NSCs extend an actin-rich primary protrusion toward the neuropil. However, function actin cytoskeleton during unknown. Here, we reveal fine F-actin structures protrusions by expansion super-resolution microscopy. We show that polymerization promotes nuclear...
Morphogen-mediated signaling is critical for proper organ development and stem cell function, well-characterized mechanisms spatiotemporally limit the expression of ligands, receptors, ligand-binding cell-surface glypicans. Here, we show that in developing Drosophila ovary, canonical Wnt promotes formation somatic escort cells (ECs) their protrusions, which establish a physical permeability barrier to define morphogen territories germ differentiation. The protrusions shield from Dpp Wingless...
Abstract Morphogen-mediated signaling is critical for proper organ development and stem cell function, well-characterized mechanisms spatiotemporally limit the expression of ligands, receptors, ligand-binding cell-surface glypicans. Here, we show that in developing Drosophila ovary, canonical Wnt promotes formation somatic escort cells (ECs) their protrusions, which establish a physical permeability barrier to define morphogen territories germ differentiation. The protrusions shield from Dpp...