A5040533395- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Immune cells in cancer
- Genetic factors in colorectal cancer
- SARS-CoV-2 detection and testing
- Pancreatic and Hepatic Oncology Research
- PI3K/AKT/mTOR signaling in cancer
- Single-cell and spatial transcriptomics
- Cancer Mechanisms and Therapy
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
Stanford University
2019-2020
The tumor microenvironment (TME) consists of a heterogenous cellular milieu that can influence cancer cell behavior. Its characteristics have an impact on treatments such as immunotherapy. These features be revealed with single-cell RNA sequencing (scRNA-seq). We hypothesized scRNA-seq analysis gastric together paired normal tissue and peripheral blood mononuclear cells (PBMC) would identify critical elements deregulation not apparent other approaches.scRNA-seq was conducted seven patients...
The diverse cellular milieu of the gastric tissue microenvironment plays a critical role in normal homeostasis and tumor development. However, few cell culture model can recapitulate intercellular signaling vitro. We used primary system to generate murine p53 null containing both epithelium mesenchymal stroma. To characterize niche signaling, we single RNA sequencing (scRNA-Seq) determine transcriptomes 4,391 individual cells. Based on specific markers, identified epithelial cells,...
Abstract Some gastric cancers have FGFR2 amplifications, making them sensitive to FGFR inhibitors. However, cancer cells inevitably develop resistance despite initial response. The underlying mechanism inhibition is unclear. In this study, we applied a kinome-wide CRISPR/Cas9 screen systematically identify kinases that are determinants of sensitivity potent inhibitor AZD4547 in KatoIII cells, cell line with amplification. total, identified 20 kinases, involved ILK, SRC, and EGFR signaling...
ABSTRACT Background The genome of SARS-CoV-2 is susceptible to mutations during viral replication due the errors generated by RNA-dependent RNA polymerases. These enable evolve into new strains. Viral quasispecies emerge from de novo that occur in individual patients. In combination, these sets provide distinct genetic fingerprints reveal patterns transmission and have utility contract tracing. Methods Leveraging thousands sequenced genomes, we performed a pangenome analysis identify...
<p>Supplementary methods, legends to supplementary figures and tables, tables</p>
<p>Supplementary methods, legends to supplementary figures and tables, tables</p>
<div>AbstractPurpose:<p>The tumor microenvironment (TME) consists of a heterogenous cellular milieu that can influence cancer cell behavior. Its characteristics have an impact on treatments such as immunotherapy. These features be revealed with single-cell RNA sequencing (scRNA-seq). We hypothesized scRNA-seq analysis gastric together paired normal tissue and peripheral blood mononuclear cells (PBMC) would identify critical elements deregulation not apparent other...
<div>AbstractPurpose:<p>The tumor microenvironment (TME) consists of a heterogenous cellular milieu that can influence cancer cell behavior. Its characteristics have an impact on treatments such as immunotherapy. These features be revealed with single-cell RNA sequencing (scRNA-seq). We hypothesized scRNA-seq analysis gastric together paired normal tissue and peripheral blood mononuclear cells (PBMC) would identify critical elements deregulation not apparent other...