A5041840523- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Genetic factors in colorectal cancer
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Microfluidic and Capillary Electrophoresis Applications
- SARS-CoV-2 and COVID-19 Research
- Genomics and Phylogenetic Studies
- MicroRNA in disease regulation
- Advanced Biosensing Techniques and Applications
- Colorectal Cancer Treatments and Studies
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Analytical Chemistry and Chromatography
- Chromosomal and Genetic Variations
- Extracellular vesicles in disease
- Colorectal Cancer Screening and Detection
- Tracheal and airway disorders
Stanford University
2017-2024
Pohang University of Science and Technology
2008-2024
Stanford Medicine
2018
Palo Alto University
2018
Zero to Three
2012
Health First
2010
Korea Post
2010
Abstract Improving the hydroxide conductivity and dimensional stability of anion exchange membranes (AEMs) while retaining their high alkaline is necessary to realize commercialization AEM water electrolysis (AEMWE). A strategy for improving AEMs by inserting fluorine atoms in core structure backbone reported, which not only reduces glass transition temperature polymer due steric strain, but also induces distinct phase separation inducing polarity discrimination facilitate formation ion...
Abstract Anion exchange membrane water electrolysis (AEMWE) has gained attention as an attractive alternative to alkaline and proton (PEMWE) due its high efficiency low hydrogen unit cost. However, the long‐term durability of AEMWE is ≈10 times lower than that PEMWE, which typically operates for 40 000 h. Here, a new design strategy presented aryl ether‐free PFPBPF‐QA anion membranes with interstitial alkyl chains in conducting groups polymer backbone. The rationally designed PFPBPF‐4‐QA,...
Abstract Microsatellites are multi-allelic and composed of short tandem repeats (STRs) with individual motifs mononucleotides, dinucleotides or higher including hexamers. Next-generation sequencing approaches other STR assays rely on a limited number PCR amplicons, typically in the tens. Here, we demonstrate STR-Seq, next-generation technology that analyses over 2,000 STRs parallel, provides accurate genotyping microsatellites. STR-Seq employs vitro CRISPR–Cas9-targeted fragmentation to...
Background Colon microbiome composition contributes to the pathogenesis of colorectal cancer (CRC) and prognosis. We analyzed 16S rRNA sequencing data from tumor samples patients with metastatic CRC determined clinical implications. Materials methods enrolled 133 at St. Vincent Hospital in Korea. The V3-V4 regions gene DNA were amplified, sequenced on an Illumina MiSeq, using DADA2 package. Results After excluding that retained <5% total reads after merging, 120 analyzed. median age...
Abstract Background The genome of SARS-CoV-2 is susceptible to mutations during viral replication due the errors generated by RNA-dependent RNA polymerases. These enable evolve into new strains. Viral quasispecies emerge from de novo that occur in individual patients. In combination, these sets provide distinct genetic fingerprints reveal patterns transmission and have utility contact tracing. Methods Leveraging thousands sequenced genomes, we performed a pangenome analysis identify...
Abstract Light‐emitting organic small molecules require high internal and external quantum efficiencies with excellent radiative characteristics for their potential application in next‐generation optoelectronics. Nonetheless, achieving efficiency solid states remains a formidable challenge, primarily owing to the non‐radiative processes. Therefore, conformational modulation is pivotal influencing emission properties mitigate decay. Notably, modifying intra‐ intermolecular non‐covalent...
Autism spectrum disorder (ASD) has been thought to have strong genetic background, but major contributing genes or associated molecular-genetic pathways are yet be identified. To explore the idiopathic ASD-associated copy number variations (CNVs), we conducted case-control study using whole-genome analysis.Whole-genome microarray-based comparative genomic hybridization was carried out on 28 children (24 boys and four girls) diagnosed as ASD 62 Korean adults (45 males 17 females) without any...
Several methods based on screening for a 16S ribosomal RNA gene marker have been developed rapid and sensitive detection of pathogenic microorganisms. One such method, CE-based SSCP (CE-SSCP), has enormous potential because the technique can separate sequence variants using simple procedure. However, conventional CE-SSCP systems limited resolution cannot most gene-specific markers unless combined with additional modification steps. A high-resolution system that uses...
Abstract Although CE‐SSCP analysis combined with 16S ribosomal RNA gene‐specific PCR has enormous potential as a simple and versatile pathogen detection technique, low resolution of causes the limited application. Among experimental conditions affecting resolution, polymer matrix is considered to be most critical improve analysis. However, due peak broadening caused by interaction between hydrophobic moiety matrices DNA, conventional are not ideal for A...
Sensitive multiplex detection methods for foodborne pathogens are important in controlling food safety, and of genetic markers is accepted to be one the best tools sensitive detection. Although CE technology offers great potential terms detection, necessary amplification confined sharing common primers such as 16 S r RNA gene. For precise pathogen‐specific genes optimal markers. ligation‐dependent probe ( MLPA ) appropriate specific markers, requirement stuffers, ensure length‐dependent...
Developing diagnostic tools based on the application of known disease/phenotype-associated copy number variations (CNVs) requires capacity to measure CNVs in a multiplex format with sufficient reliability and methodological simplicity. In this study, we developed reliable user-friendly CNV detection method, termed stuffer-free MLPA-CE-SSCP, that combines variation ligation-dependent probe amplification (MLPA) CE-SSCP. variation, MLPA probes were designed without conventionally required...
Abstract Rapid and simple analysis for the multiple target pathogens is critical patient management. CE‐SSCP on a microchip provides high speed, sensitivity, portable genetic platform in molecular diagnostic fields. The capability of separating ssDNA molecules capillary electrophoretic microchannel with resolution issue to perform precise interpretation electropherogram. In this study, we explored potential poly(ethyleneoxide)‐poly(propyleneoxide)‐poly(ethyleneoxide) (PEO‐PPO‐PEO) triblock...
Abstract The human genome is composed of two haplotypes, otherwise called diplotypes, which denote phased polymorphisms and structural variations (SVs) that are derived from both parents. Diplotypes place genetic variants in the context cis-related a diploid genome. As result, they provide valuable information about hereditary transmission, SV, regulation gene expression other features informative for understanding genetics. Successful diplotyping with short read whole sequencing generally...
Rapid diagnosis of bacterial infection is important for patient management and appropriate therapy during the early phase bacteria-induced disease. Among existing techniques identifying microbial, CE-SSCP combined with 16S ribosomal RNA gene-specific PCR has benefits excellent sensitivity, resolution, reproducibility. However, even though can separate products high-resolution, multiplex detection quantification are complicated by primer-dimer formation non-specific amplification. Here, we...
Expression profiling of multiple microRNAs (miRNAs) generally provides valuable information for understanding various biological processes. Thus, it is necessary to develop a sensitive and accurate miRNA assay suitable multiplexing. Isothermal exponential amplification reaction (EXPAR) has received significant interest as an analysis method because high efficiency. However, EXPAR cannot be used broader range applications owing limitations such complexity probe design lack proper detection...
We developed a generally applicable method, CRISPR/Cas9-targeted long-read sequencing (CTLR-Seq), to resolve, haplotype-specifically, the large and complex regions in human genome that had been previously impenetrable analysis, such as segmental duplications (SegDups) their associated rearrangements. CTLR-Seq combines vitro Cas9-mediated cutting of pulse-field gel electrophoresis isolate intact (i.e., up 2,000 kb) genomic encompass unresolvable sequences. These targets are then sequenced...
Quantum dot solar cells (QDSCs) have excellent optoelectronic properties but face challenges in performance and stability. This review highlights advances organic conjugated materials to improve QDSC outlines key design principles.
Abstract Conventional antimicrobial activity analyses such as the broth dilution method and disk diffusion test are considerably demanding processes for new agent discovery sensitive diagnosis of infectious diseases. Here, we developed a analysis system using CE‐based SSCP (CE‐SSCP) combined with 16S rRNA gene‐specific PCR (PCR/CE‐SSCP). Using this method, population change in microbial community response to specific agents could be quantified high sensitivity accuracy from small sample...
Accuracy, simplicity, and cost‐effectiveness are the most important criteria for a genotyping method SNPs compatible with clinical use. One developed SNP genotyping, ligase‐based discrimination, is considered simplest diagnosis. However, multiplex assays using this limited by detection method. Although CE has been introduced as an alternative to error prone microarray‐based detection, design process assay procedure complicated because of DNA size‐dependent separation principle. In study, we...
Abstract Small RNAs that originate from transfer RNA (tRNA) species, tRNA-derived fragments (tRFs), play diverse biological functions but little is known for their association with aging. Moreover, biochemical aspects of tRNAs limit discovery functional tRFs by high throughput sequencing. In particular, genes encoding exist as multiple copies throughout genome, and mature have various modified bases, contributing to ambiguities sequencing-based analysis tRFs. Here, we report age-dependent...