- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Power Systems and Renewable Energy
- Plant Virus Research Studies
- Microgrid Control and Optimization
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Frequency Control in Power Systems
Czech Academy of Sciences, Institute of Experimental Botany
2016-2024
Palacký University Olomouc
2019
Abstract Genetic information in the cell nucleus controls organismal development and responses to environment, finally ensures its own transmission next generations. To achieve so many different tasks, genetic is associated with structural regulatory proteins, which orchestrate nuclear functions time space. Furthermore, plant life strategies require chromatin plasticity allow a rapid adaptation abiotic biotic stresses. Here, we summarize current knowledge on organization of dynamics...
Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend accumulate significant reserves during maturation, including a population of stored mRNAs, that are utilized later for massive translation various proteins growing tubes. Here, we performed thorough transcriptomic and proteomic analysis translated transcripts three subcellular compartments...
Abstract Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid gametophyte. This demands synchronized gene expression short developmental window to ensure double fertilization seed set. While transcriptomic changes developing pollen are known for Arabidopsis , no studies have integrated RNA proteomic data this model. Further, role alternative splicing has not been fully addressed, yet post-transcriptional post-translational...
Translation is a fundamental process for every living organism. In plants, the rate of translation tightly modulated during development and in response to environmental cues. However, it difficult measure actual state tissues vivo. Here, we report implementation an vivo marker based on bimolecular fluorescence complementation, Ribo-BiFC. We combined method originally developed fruit-fly with improved low background split-mVenus BiFC system previously described plants. labelled Arabidopsis...
Abstract Pollen germination and pollen tube (PT) growth are extremely sensitive to high temperatures. During heat stress (HS), global translation shuts down favors the maintenance of essential cellular proteome for cell viability protection against protein misfolding. Here, we demonstrate that under normal conditions, Arabidopsis (Arabidopsis thaliana) eukaryotic initiation factor subunit eif3m1/eif3m2 double mutant exhibits poor germination, loss PT integrity an increased rate aborted...