A5114376755- Immune Response and Inflammation
- Endoplasmic Reticulum Stress and Disease
- Blood disorders and treatments
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Lipid Membrane Structure and Behavior
- Chemical Synthesis and Analysis
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- Sphingolipid Metabolism and Signaling
- Radiopharmaceutical Chemistry and Applications
- Liver Disease Diagnosis and Treatment
- Lysosomal Storage Disorders Research
- Pancreatic function and diabetes
- Lipid metabolism and biosynthesis
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2023
Polish Academy of Sciences
2017-2019
Bacterial lipopolysaccharide (LPS) induces strong pro-inflammatory reactions after sequential binding to CD14 protein and TLR4 receptor. Here we show that controls generation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] in response LPS binding. In J774 cells HEK293 expressing exposed 10-100 ng/ml LPS, the level PI(4,5)P2 rose a biphasic manner with peaks at 5-10 min 60 min. After stimulation, underwent prominent clustering plasma membrane accompanied by accumulation type I...
Lipopolysaccharide (LPS) is the component of Gram-negative bacteria that activates Toll-like receptor 4 (TLR4) to trigger proinflammatory responses. We examined involvement Lyn tyrosine kinase in TLR4 signaling macrophages, distinguishing its catalytic activity and intermolecular interactions. For this, a series Lyn-GFP constructs bearing point mutations particular domains were overexpressed RAW264 macrophage-like cells or murine peritoneal their influence on LPS-induced responses was...
Palmitic acid (C16:0) is the most abundant saturated fatty in animals serving as a substrate synthesis and β-oxidation of other lipids, modification proteins called palmitoylation. The influence dietary palmitic on protein S-palmitoylation remains largely unknown. In this study we performed high-throughput proteomic analyses membrane-enriched fraction murine liver to examine palm oil-rich diet (HPD) proteins. HPD feeding for 4 weeks led an accumulation C16:0 C18:1 acids livers which...
Diacylglycerol kinase-ε (DGKε) phosphorylates DAG to phosphatidic acid with unique specificity toward 18:0/20:4 (SAG). SAG is a typical backbone of phosphatidylinositol and its derivatives, therefore DGKε activity crucial for the turnover these signaling lipids. Malfunction contributes several pathophysiological conditions, including atypical hemolytic uremic syndrome (aHUS) linked DGKE mutations. In present study we analyzed role zinc finger motif C1B domain DGKε, as some aHUS-linked...
Diacylglycerol kinase-ε (DGKε) catalyzes phosphorylation of diacylglycerol to phosphatidic acid with a unique specificity toward 1-stearoyl-2-arachidonoyl-sn-glycerol which is backbone phosphatidylinositol (PI). Owing this specificity, DGKε involved in the PI cycle maintaining cellular level phosphorylated derivatives signaling activity, and was also found crucial for lipid metabolism. dysfunction linked development atypical hemolytic uremic syndrome possibly other human diseases. Despite...
Abstract Diacylglycerol kinase-ε (DGKε) catalyzes phosphorylation of diacylglycerol to phosphatidic acid with a unique specificity toward 1-stearoyl-2-arachidonoyl- sn -glycerol which is backbone phosphatidylinositol (PI). Owing this specificity, DGKε involved in the PI cycle maintaining cellular level phosphorylated derivatives signaling activity, and was also found crucial for lipid metabolism. dysfunction linked development atypical hemolytic uremic syndrome possibly other human diseases....