A5101717125- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Sphingolipid Metabolism and Signaling
- Protein Structure and Dynamics
- Air Quality and Health Impacts
- Indoor Air Quality and Microbial Exposure
- Adipose Tissue and Metabolism
- Gene expression and cancer classification
- Hemoglobin structure and function
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Nutritional Studies and Diet
- Smoking Behavior and Cessation
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Heme Oxygenase-1 and Carbon Monoxide
- Inflammation biomarkers and pathways
- Health, Environment, Cognitive Aging
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
Philip Morris International (Switzerland)
2020-2024
Max Planck Institute of Molecular Cell Biology and Genetics
2015-2020
Significance The function of the cell membrane as a barrier and matrix for biochemical activity relies on properties imparted by lipids. In eukaryotes, sterols are crucial modulating molecular order membranes. Sterol ordering provides basis lateral segregation promotes fluid, mechanically robust plasma membrane. How do organisms that lack determine order? Hopanoids bacterial lipids have been demonstrated to sterol-like in vitro. We now explore distribution hopanoids their effect membranes...
Cells, from microbes to mammals, adapt their membrane lipid composition in response environmental changes maintain optimal properties. Global patterns of lipidome remodeling are poorly understood, particularly organisms with simple compositions that can provide insight into fundamental principles adaptation. Using shotgun lipidomics, we examine the yet, as show here, adaptive plant-associated Gram-negative bacterium Methylobacterium extorquens. We observe minimally 11 lipids account for 90%...
Cigarette smoke (CS) causes adverse health effects and, for smoker who do not quit, modified risk tobacco products (MRTPs) can be an alternative to reduce the of developing smoking-related diseases. Standard toxicological endpoints lack sensitivity, with systems toxicology approaches yielding broader insights into mechanisms. In a 6-month study on ApoE−/− mice, we conducted integrative multi-omics analysis assess aerosols from Carbon Heated Tobacco Product (CHTP) 1.2 and Heating System (THS)...
Smoking is an important risk factor for the development of chronic obstructive pulmonary disease and cardiovascular diseases. This study aimed to further elucidate role ceramides, as a key lipid class dysregulated in states. In this article we developed validated LC–MS/MS method ceramides (Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/24:0) Cer(d18:1/24:1(15Z)) absolute quantification. We deployed it together with proteomics transcriptomic analysis assess effects cigarette smoke (CS) from...
Abstract Cigarette smoking is the major cause of chronic obstructive pulmonary disease. Considerable attention has been paid to reduced harm potential nicotine-containing inhalable products such as electronic cigarettes (e-cigarettes). We investigated effects mainstream cigarette smoke (CS) and e-vapor aerosols (containing nicotine flavor) generated by a capillary aerosol generator on emphysematous changes, lung function, molecular alterations in respiratory system female Apoe −/− mice. Mice...
Abstract Cigarette smoking is a major preventable cause of morbidity and mortality. While quitting the best option, switching from cigarettes to non-combustible alternatives (NCAs) such as e-vapor products viable harm reduction approach for smokers who would otherwise continue smoke. A key challenge clinical assessment NCAs that self-reported product use can be unreliable, compromising proper evaluation their risk potential. In this cross-sectional study 205 healthy volunteers, we combined...
Abstract Cells, from microbes to man, adapt their membranes in response the environment maintain properties and functions. To adapt, lipid composition is homeostatically regulated conserve optimal membrane properties. Global patterns of lipidome remodelling are poorly understood, particularly model organisms with simple compositions that can provide insight into fundamental principles underlying adaptation. Using shotgun lipidomics, we examined yet adaptive plant-associated Gram-negative...
Cells, from microbes to man, adapt their membranes in response the environment maintain properties and functions. To adapt, lipid composition is homeostatically regulated conserve optimal membrane properties. Global patterns of lipidome remodelling are poorly understood, particularly model organisms with simple compositions that can provide insight into fundamental principles underlying adaptation. Using shotgun lipidomics, we examined yet adaptive plant-associated Gram-negative bacterium...
Lipids play a vital role as essential components of all prokaryotic and eukaryotic cells. Constant technological improvements in mass spectrometry have made lipidomics powerful analytical tool for monitoring tissue lipidome compositions homeostatic well disease states. This paper presents step-by-step protocol shotgun lipid analysis method that supports the simultaneous detection quantification few hundred molecular species different biofluid samples at high throughput. leverages automated...
Lipids play a vital role as essential components of all prokaryotic and eukaryotic cells. Constant technological improvements in mass spectrometry have made lipidomics powerful analytical tool for monitoring tissue lipidome compositions homeostatic well disease states. This paper presents step-by-step protocol shotgun lipid analysis method that supports the simultaneous detection quantification few hundred molecular species different biofluid samples at high throughput. leverages automated...