A5101669706- Lysosomal Storage Disorders Research
- Systemic Lupus Erythematosus Research
- RNA regulation and disease
- Cellular transport and secretion
- Oral microbiology and periodontitis research
- Autoimmune Neurological Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Salivary Gland Disorders and Functions
- Genetics and Neurodevelopmental Disorders
- Neurological diseases and metabolism
- Health, Environment, Cognitive Aging
- Plant Disease Resistance and Genetics
- Forest Biomass Utilization and Management
- SARS-CoV-2 detection and testing
- Insect Pheromone Research and Control
- Protein purification and stability
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Outbreaks Research
- Atherosclerosis and Cardiovascular Diseases
- Photodynamic Therapy Research Studies
- Bone health and treatments
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Fluoride Effects and Removal
University of Rochester Medical Center
1995-2024
University of Rochester
1993-2019
Atkins (United States)
2005
Institute of Biomedical Science
2004
Naturally occurring strains of Candida albicans are opportunistic pathogens that lack a sexual cycle and usually diploids with eight pairs chromosomes. C. spontaneously gives rise to high frequency colonial morphology mutants altered electrophoretic karyotypes, involving one or more their However, the most frequent changes involve chromosome VIII, which contains genes coding for ribosomal DNA (rDNA) units. We have used restriction fragment lengths analyze number physical array rDNA units on...
Streptococcus rattus FA-1 and sanguis NCTC 10904 underwent phenotypic acid adaptation in batch cultures toward the end of sugar-fueled growth after culture pH had dropped to triggering values. The bacteria could be derepressed or induced for arginine deiminase independently adaptation, arginolysis afforded protection against killing over above that adaptation.
Autoantibodies to glutamic acid decarboxylase (GAD65) have been reported in sera from the Cln3 –/– mouse model of juvenile neuronal ceroid lipofuscinosis (JNCL), and individuals with this fatal paediatric neurodegenerative disorder. To investigate existence other circulating autoreactive antibodies, we used patients JNCL forms (NCL) as primary antisera stain rat human central nervous system sections. displayed characteristic patterns IgG, but not IgA, IgE or IgM immunoreactivity that was...
Abstract Background and Objective The primary therapy for deep tissue abscesses is drainage accompanied by systemic antimicrobial treatment. However, the long antibiotic course required increases probability of acquired resistance, high incidence polymicrobial infections in complicates treatment choices. Photodynamic (PDT) effective against multiple classes organisms, including those displaying drug may serve as a useful adjunct to standard care reduction abscess microbial burden following...
Abstract Cigarette smoking is a major preventable cause of morbidity and mortality. While quitting the best option, switching from cigarettes to non-combustible alternatives (NCAs) such as e-vapor products viable harm reduction approach for smokers who would otherwise continue smoke. A key challenge clinical assessment NCAs that self-reported product use can be unreliable, compromising proper evaluation their risk potential. In this cross-sectional study 205 healthy volunteers, we combined...
Fluoride at concentrations greater than 0.01 mM was found to be a quasi-irreversible inhibitor of enolase permeabilized cells Streptococcus mutans GS-5 and also isolated yeast enolase. The inhibition appeared the type that has been described for P-ATPases, but not dependent on added Al3+ or Be2+ ions. reversed by repeatedly washing in chilled fluoride-free medium could product, phosphoenolpyruvate, very high levels substrate, 2-phosphoglycerate. Irreversible glycolysis evident after fluoride...
Infantile and juvenile neuronal ceroid lipofuscinosis (NCLs) are progressive neurodegenerative disorders of childhood with distinct ages clinical onset, but a similar pathological outcome. NCL inherited in an autosomal recessive manner due to mutations the CLN1 CLN3 genes, respectively. Recently developed Cln1- Cln3-knockout mouse models share similarities pathology respective human disease. Using oligonucleotide arrays we identified reproducible changes gene expression brains both...
The pathogenic mechanisms underlying Batten disease are unclear. Patients uniformly possess autoantibodies against glutamic acid decarboxylase (GAD) that predominantly reactive with a region of GAD (amino acids 1 to 20) distinct from subjects autoimmune type diabetes or stiff-person syndrome. patients did not other diabetes-associated autoantigens and human leukocyte antigen genotypes revealed no specific associations this disease.
purpose. Juvenile neuronal ceroid lipofuscinosis (JNCL or Batten Disease) is one of the most common progressive neurodegenerative disorders childhood, resulting from autosomal recessive inheritance mutations in CLN3 gene. Pathologically, disease characterized by lysosomal storage autofluorescent material all tissue types. Although seizures, mental retardation, and loss motor skills, first presenting symptom vision loss. methods. High-density oligonucleotide arrays were used to profile...
Parabens were found to be potent inhibitors of alkali production from arginine by oral streptococci such as Streptococcus rattus, sanguis and gordonii. For example, 2 mumol butylparaben per ml completely irreversibly inhibited arginolysis intact cells S. rattus FA-1 was lethal for the organism. In contrast, not a very effective inhibitor ureolysis salivarius 57.I, although it did kill cells. Butylparaben cytoplasmic enzymes deiminase, carbamate kinase urease in permeabilized or isolated...
1‐Hydroxyethane‐1, 1‐diphosphonate (EHDP) and a variety of other diphosphonates, also pyrophosphate, at millimolar levels were found to inhibit the growth Streptococcus mutans GS‐5. Inhibition appeared be due mainly chelation Mg 2+ could readily reversed through addition , or less effectively, by divalent cations. The trianionic forms diphosphonates pyrophosphate more effective inhibitors than dianionic forms. Diphosphonates did not glycolysis S. assayed in terms glucose utilization,...
Discoid lupus erythematosus (DLE) is a severely disfiguring and difficult to treat autoimmune skin disease for which no new therapies have been FDA-approved in over 60 years. One potential therapeutic target cutaneous B cells. Though rare healthy skin, cells are prevalent DLE lesions, comprising 10-20% of the robust lymphocytic infiltrate. However, phenotype function this expanded cell population not evaluated detail it unknown whether play role pathogenesis. We analyzed T (CD3), (CD20),...