A5076796719- Iron Metabolism and Disorders
- Trace Elements in Health
- Bioinformatics and Genomic Networks
- Fungal and yeast genetics research
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Drug Transport and Resistance Mechanisms
- Genomics and Chromatin Dynamics
- Heavy Metal Exposure and Toxicity
- Orthopaedic implants and arthroplasty
- RNA regulation and disease
- Caveolin-1 and cellular processes
- Galectins and Cancer Biology
- Lipoproteins and Cardiovascular Health
- Plant Gene Expression Analysis
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Atherosclerosis and Cardiovascular Diseases
- Barrier Structure and Function Studies
- Folate and B Vitamins Research
Linkou Chang Gung Memorial Hospital
2022
Chang Gung University
2022
University of Wisconsin–Madison
2014-2020
Madison Group (United States)
2020
Newcastle University
2019
California State University, Fullerton
2007-2010
Abstract Stressed cells coordinate a multi‐faceted response spanning many levels of physiology. Yet knowledge the complete stress‐activated regulatory network as well design principles for signal integration remains incomplete. We developed an experimental and computational approach to integrate available protein interaction data with gene fitness contributions, mutant transcriptome profiles, phospho‐proteome changes in responding salt stress, infer salt‐responsive signaling yeast. The...
Ionic copper entering blood plasma binds tightly to albumin and the macroglobulin transcuprein. It then goes primarily liver kidney except in lactation, where a large portion directly mammary gland. Little is known about how this taken up from these proteins. To examine this, kinetics of uptake purified human alpha(2)-macroglobulin, effects inhibitors, were measured using hepatic (HepG2) epithelial (PMC42) cell lines. At physiological concentrations (3-6 muM), both types took proteins...
Stress tolerance and rapid growth are often competing interests in cells. Upon severe environmental stress, many organisms activate defense systems concurrent with arrest. There has been debate as to whether aspects of the stress-activated transcriptome regulated by stress or an indirect byproduct reduced proliferation. For example, stressed Saccharomyces cerevisiae cells mount a common gene expression program called response (ESR) [1Gasch A.P. Spellman P.T. Kao C.M. Carmel-Harel O. Eisen...
Significance Stage-specific and gene-specific molecular machines are recruited to elongating RNA polymerase II (Pol II) through reversible phosphorylation of its carboxyl-terminal domain. This unusual domain is composed a tandemly repeating Y 1 S 2 P 3 T 4 5 6 7 motif. Here, we identify class noncoding that relies on phospho-Thr4 for effective termination. We also protein complexes rely Thr4 associate with Pol II. Rtt103, one the proteins engages phospho-Thr4, binds phospho-Ser2 facilitates...
Iron regulatory proteins (IRPs) are iron-responsive RNA binding that dictate changes in cellular iron metabolism animal cells by controlling the fate of mRNAs containing responsive elements (IREs). IRPs have broader physiological roles as some targeted encode with functions beyond suggesting hierarchical regulation IRP-targeted mRNAs. We observe translational encoding storage (L- and H-ferritins) export (ferroportin) different set-points responsiveness compared to for TCA cycle enzyme...
Abstract Background In endothelial cells, phospholipase C (PLC) β1-activated Ca 2+ is a crucial second messenger for the signaling pathways governing angiogenesis. PLCβ1 inactivated by complexing with an intracellular protein called translin-associated factor X (TRAX). This study demonstrates specific interactions between Globo H ceramide (GHCer) and TRAX, which highlight new angiogenic control through activation. Methods Globo-series glycosphingolipids (GSLs), including GHCer stage-specific...
Normally Cu(II) entering the blood plasma binds tightly to albumin (Alb) and macroglobulin, transcuprein. However, little is known about how this copper then taken up by hepatic cells, which are main recipients. Two determine this, kinetics of uptake from these proteins, effects inhibitors, were measured in human (HepG2) mammary epithelial (PMC42) cell lines. At physiological concentrations (1–5 uM) both types took Cu purified Alb alpha‐2‐macroglobulin (a2M) at rates similar each other those...
As first shown in rats, the uptake of Cu by mammary gland increases about 20‐fold over that virgin state lactation, after i.p. or i.v. injection tracer 64 Cu. We hypothesized this was due to upregulation transporters and/or systems/processes induced lactational hormones. This tested PMC42 cell model human breast, which responds hormones production milk proteins and trafficking pumps, ATP7A B; as well mouse explants exposed delivered cells grown bicameral chambers either pure albumin,...
Our long term goal is to understand exactly how Cu taken up by cells from its blood plasma carriers and this regulated in lactation. To investigate the possibility that DMT1 might at least partly be involved, we compared organ distribution of tracer 64 Cu(II) 1h after injection i.p. into non‐lactating lactating Belgrade rats (with defective DMT1) their wild type (Fisher) counterparts. No differences were observed. Both kinds nonlactating had ~70% administered liver kidney. Two‐5 days...
Albumin, with a high affinity Cu binding site at its N-terminus, is one of two main components the exchangeable plasma pool. Thus during lactation, when rapidly enters mammary gland and milk, we would expect it to deliver cells in that organ. We thus examined kinetics uptake efficiency delivered by albumin polarized human breast cell monolayers (PMC42), model for epithelium. For this, was purified from Cibacron Blue pseudoaffinity chromatography. Residual removed histidine. Pure loaded...
Emergence of resistance to molecular targeted therapy constitutes a potential limitation clinical benefits in cancer treatment. Cross-resistance commonly happens with chemotherapeutic agents but might not agents. In the current study, TP53 wild -type cell lines druggable MAPK pathway mutations (BRAFV600E (WM35) or NRAS Q61K (SJSA-1)) were compared their mutant sublines (WM35-R, SN40R2) derived by selection for MDM2/p53 binding antagonists. The continued presence targets (TP53MUT) WM35-R and...
Advances in systems biology have made clear the importance of network models for capturing knowledge about complex relationships gene regulation, metabolism, and cellular signaling. A common approach to uncovering biological networks involves performing perturbations on elements network, such as knockdown experiments, measuring how perturbation affects some reporter process under study. In this article, we develop context-specific nested effects (CSNEMs), an inferring that generalizes...