A5030785077- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Iron Metabolism and Disorders
- Drug Transport and Resistance Mechanisms
- Heavy metals in environment
- Biocrusts and Microbial Ecology
- Chromium effects and bioremediation
- Neuroendocrine Tumor Research Advances
- Photosynthetic Processes and Mechanisms
- Corrosion Behavior and Inhibition
- Concrete Corrosion and Durability
- Advanced Breast Cancer Therapies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Phase Equilibria and Thermodynamics
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Energy and Environment Impacts
- Lung Cancer Research Studies
- Thermodynamic properties of mixtures
- Membrane Separation and Gas Transport
- Gestational Diabetes Research and Management
- Aluminum toxicity and tolerance in plants and animals
- Digestive system and related health
- Animal Genetics and Reproduction
- Chronic Lymphocytic Leukemia Research
Deakin University
2013-2023
Centre for Cellular and Molecular Biology
1999-2013
California State University, Fullerton
2009
Max Planck Institute of Molecular Physiology
2008
Max Planck Society
2008
In-Q-Tel
2001
Ionic copper entering blood plasma binds tightly to albumin and the macroglobulin transcuprein. It then goes primarily liver kidney except in lactation, where a large portion directly mammary gland. Little is known about how this taken up from these proteins. To examine this, kinetics of uptake purified human alpha(2)-macroglobulin, effects inhibitors, were measured using hepatic (HepG2) epithelial (PMC42) cell lines. At physiological concentrations (3-6 muM), both types took proteins...
Copper deficiency during pregnancy results in early embryonic death and foetal structural abnormalities including skeletal, pulmonary cardiovascular defects. During pregnancy, copper is transported from the maternal circulation to foetus by mechanisms which have not been clearly elucidated. Two copper-transporting ATPases, Menkes (ATP7A; MNK) Wilson (ATP7B; WND), are expressed placenta both involved placental transport, as accumulates disease. The regulatory of MNK WND their exact role...
Environmental context Soils contaminated with metals can pose both environmental and human health risks. This study showed that a common crop vegetable grown in the presence of cadmium zinc readily accumulated these metals, thus could be source toxicity when eaten. The work highlights potential risks from consuming crops on soils. Abstract Ingestion plants heavy metal soils cause because accumulation. We compared Cd Zn levels Brassica rapa, widely vegetable, hyperaccumulator Solanum nigrum...
Toxic milk (tx) is a copper disorder of mice that causes hepatic accumulation similar to seen in patients with Wilson disease. Both disorders are caused by defect the ATP7B copper-transporting ATPase. A feature tx phenotype production copper-deficient lactating dams homozygous for mutation; lethal pups. It has not been determined whether direct consequence impaired expression protein mammary gland. With use immunohistochemistry, our study demonstrated was mislocalized mouse gland, which...
Zinc is an essential trace element required by all living organisms. An adequate supply of zinc particularly important in the neonatal period. a significant component breast milk, which transported across maternal epithelia during lactation. The mechanisms becomes constituent milk have not been elucidated. function transporter ZnT4 transport into lactation was previously demonstrated studies mouse mutant, ‘lethal milk’ mouse, where mutation gene decreased milk. In present study, we...
Background/Aims: The copper transporting ATPases, Menkes (ATP7A; MNK) and Wilson (ATP7B; WND) are essential for normal transport in the human body. placenta is key organ supply to fetus during pregnancy it one of few organs body express both ATPases. therefore provides a unique opportunity elucidate specific roles these transporters within cell type. Methods/Results: Using polarized placental Jeg-3 cells, siRNA technology radio-labelled <sup>64</sup>Cu assays, MNK WND were shown have...
A role for the copper transporter, ATP7B, in secretion of from human breast into milk has previously not been reported, although it is known that murine ortholog ATP7B facilitates mouse mammary gland. We show here expressed luminal epithelial cells both resting and lactating breast, where a perinuclear localization diffuse location tissue. protein was present different subset vesicles those containing proteins did overlap with Menkes ATPase, ATP-7A, except region cells. In cultured line,...
Docosahexaenoic acid (DHA) is the major polyunsaturated fatty in neuronal cell membranes. We hypothesize that DHA induces a decrease death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA‐deficient medium increased levels active caspase‐3, relative DHA‐replete cells, confirming adverse effects deficiency promoting death. In DHA‐treated uptake was 65% less mRNA protein were comparison with DHA‐depleted cells. propose neuroprotective function exerted reduction...
Metal homoeostasis in cyanobacteria is based on uptake and export systems that are controlled by their own regulators. This study characterises the zinc (Znu) system Nostoc punctiforme. The was found to comprise of three subunits an ACB operon: a Zn(2+)-binding protein (ZnuA18), transmembrane domain (ZnuB) ATPase (ZnuC). These proteins encoded within znu operon regulated transcription repressor (Zur). Interestingly, second (ZnuA08) also identified at distal genomic location. Interactions...
Lifestyle factors mediate epigenetic changes that can cause chronic diseases. Although animal and laboratory studies link to diabetes, information in women with gestational diabetes (GDM) type 2 is lacking.This study sought measure markers across pregnancy early postpartum identify could be used as predictors for conversion from GDM diabetes.Global histone H3 dimethylation was measured white blood cells at three time points: 30 wk gestation, 8-10 postpartum, 20 four groups of without...
The cellular effects of biodiesel emissions particulate matter (BDEP) and petroleum diesel (PDEP) were compared using a human airway cell line, A549. At concentrations 25 microg/ml, induced the formation multinucleate cells. In cells treated with mixture 80% PDEP:20% BDEP, 52% only 16% 20% PDEP:80% BDEP background rate 7%. These results demonstrate causal relation between exposure to exhaust matter, in particular exhaust. Exposure A549 PDEP apoptosis, seen by active caspase-3 expression...
The apical cytoplasm of airway epithelium (AE) contains abundant labile zinc (Zn) ions that are involved in the protection AE from oxidants and inhaled noxious substances. A major question is how dietary Zn traffics to this compartment. In rat airways, vivo selenite autometallographic (Se-AMG)-electron microscopy revealed Zn-selenium nanocrystals structures resembling secretory vesicles cytoplasm. This observation was consistent with starry-sky Zinquin fluorescence staining confined same...
The arrangement of microtubules in soybean ( Glycine max ) cells was examined during compatible and incompatible interactions hypocotyls cv. Harosoy (susceptible) Haro 1272 (resistant) with race 1 the soybean‐specific pathogen Phytophthora sojae . Both reaction types were similar first 3 h after zoospore inoculation terms number penetrated, depth penetrated into cortex. By postinoculation, clear differences had developed between two interaction types: characterized by a hypersensitive...
Two P-type ATPases, MNK and WND were recently shown to be defective in the human disorders of copper transport, Menkes disease Wilson respectively. These proteins are important homeostasis but their full physiological function has not been established. This study uses breast carcinoma line, PMC42, investigate transport mammary gland. Northern blot analysis indicated that both mRNA expressed these cells. Western with an MNK-specific antibody demonstrated a band approx. 178 kDa, close expected...
ABSTRACT Zinc homeostasis was investigated in Nostoc punctiforme . Cell tolerance to Zn 2+ over 14 days showed that ZnCl 2 levels above 22 μM significantly reduced cell viability. After 3 , ca. 12% of the an EDTA-resistant component, suggesting intracellular localization. Zinquin fluorescence detected within cells exposed concentrations up 37 relative 0 treatment. Radiolabeled 65 uptake increased a 3-day period, while efflux occurred more rapidly 3-h time period. Four putative genes involved...
The Menkes copper ATPase (MNK) is a efflux that involved in homeostasis. Little known about the intracellular localization and cell-specific function of MNK human tissues. To investigate possible role for this protein lactation, we measured its expression sections tissue from nonlactating lactating breast. Western blot analysis showed was greater than tissue. By confocal immunofluorescence, detected luminal epithelial cells alveoli ducts but not myoepithelial cells. In breast cells, had...