A5051144934- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Retinal Diseases and Treatments
- Paleontology and Stratigraphy of Fossils
- Glaucoma and retinal disorders
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Geological and Geophysical Studies
- Geology and Paleoclimatology Research
- Isotope Analysis in Ecology
- Cellular transport and secretion
- Barrier Structure and Function Studies
- Intraocular Surgery and Lenses
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Marine and fisheries research
- Gene Regulatory Network Analysis
- Platelet Disorders and Treatments
- Advanced biosensing and bioanalysis techniques
- Calcium signaling and nucleotide metabolism
- Retinal Imaging and Analysis
- Single-cell and spatial transcriptomics
- Coastal and Marine Management
- Hematopoietic Stem Cell Transplantation
Cell and Gene Therapy Catapult
2020-2024
Indiana University – Purdue University Indianapolis
2023
Georgia Institute of Technology
2023
Indiana University
2023
Tokyo Institute of Technology
2023
University of Indianapolis
2023
University of Southampton
2023
Cell Therapy Catapult
2021
Guy's Hospital
2021
University College London
2009-2019
Transformation of somatic cells with a set embryonic transcription factors produces the pluripotent properties stem (ESCs). These induced (iPS) have potential to differentiate into any cell type, making them source from which produce as therapeutic platform for treatment wide range diseases. In many forms human retinal disease, including age-related macular degeneration (AMD), underlying pathogenesis resides within support retina, pigment epithelium (RPE). As monolayer critical photoreceptor...
Choroideremia (CHM) is an X-linked chorioretinal dystrophy that caused by mutations within a single gene, CHM Currently no effective treatment exists for these patients. Since over 30% of patients harbour nonsense in CHM, suppression therapy using translational readthrough inducing drugs may provide functional rescue REP1, thus attenuating progressive sight loss. Here, we employed two model systems to systematically test the efficacy and safety ataluren (PTC124) its novel analog PTC-414: (1)...
Inherited retinal dystrophies are an important cause of blindness, for which currently there no effective treatments. In order to study this heterogeneous group diseases, adequate disease models required in better understand pathology and test potential therapies. Induced pluripotent stem cells offer a new way recapitulate patient specific diseases vitro, providing almost limitless amount material study. We used fibroblast-derived induced generate pigment epithelium (RPE) from individual...
Abstract Human embryonic stem cells (hESCs) are a promising source of retinal pigment epithelium (RPE) cells: that can be used for the treatment common and incurable forms blindness, such as age-related macular degeneration. Although most hESC lines will produce number clusters pigmented RPE within 30–50 days when allowed to spontaneously differentiate, timing efficiency differentiation is highly variable. This could prove problematic in design robust processes large scale production cell...
Autosomal dominant vitreoretinochoroidopathy (ADVIRC) is a rare, early-onset retinal dystrophy characterised by distinct bands of circumferential pigmentary degeneration in the peripheral retina and developmental eye defects. ADVIRC caused mutations Bestrophin1 (BEST1) gene, which encodes transmembrane protein thought to function as an ion channel basolateral membrane pigment epithelial (RPE) cells. Previous studies suggest that phenotype results from alternative splicing BEST1 pre-mRNA....
Choroideremia (CHM) is an x-linked recessive chorioretinal dystrophy, with 30% caused by nonsense mutations in the CHM gene resulting in-frame premature termination codon (PTC). Nonsense-mediated mRNA decay (NMD) cell's natural surveillance mechanism that detects and destroys PTC-containing transcripts, UPF1 being central NMD modulator. efficiency can be variable amongst individuals some transcripts escaping destruction, leading to production of a truncated non-functional or partially...
The presence of residual undifferentiated pluripotent stem cells (PSCs) in PSC-derived cell therapy products (CTPs) is a major safety issue for their clinical application, due to the potential risk tumor formation. An international multidisciplinary multisite study evaluate droplet digital PCR (ddPCR) approach detect PSCs CTPs was conducted as part Health and Environmental Sciences Institute Cell Therapy-TRAcking, Circulation & Safety Technical Committee. To use ddPCR quantifying iPSCs...
The evolution of land plants in terrestrial environments brought about one the most dramatic shifts history Earth system — birth modern soils and likely stimulated massive changes marine biogeochemistry climate. In particular, multiple mass extinctions characterized by widespread anoxia, including Late Devonian extinction around 375 million years ago (Ma), may have been linked to nutrient release driven newly-rooted landscapes. Here, we use recently generated constraints from...